FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences638943
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT23650.37014256357765873No Hit
TCCATAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG20610.32256398458078417No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG19080.29861818659880457No Hit
GTTCCGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA18920.296114050862127No Hit
ATCCTAAACTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT18830.2947054745102458No Hit
ATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATTTACAT15310.23961448830333848No Hit
GTTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG14570.22803286052120458No Hit
ATAAAATACCAGCCTTCCATAGAGTATGTAAATAATGCCTAGTTTGGAAG14100.22067696179471408No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT13600.21285153761759654No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT13370.2092518424961225No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA13330.20862580856195312No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA13000.20346102860505552No Hit
CTTATATAAAATACCAGCCTTCCATAGAGTATGTAAATAATGCCTAGTTT12530.1961051298785651No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12200.1909403499216675No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG12030.18827970570144756No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT11350.1776371288205677No Hit
GTTAACATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCCTG11310.17701109488639832No Hit
GTCTGTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA11050.1729418743142972No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11000.17215933189658547No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10600.16589899255489143No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG9950.15572594112463867No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC9850.15416085628921516No Hit
CTCTATGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGTAGCGCC9430.14758749998043644No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA9240.14461383879313178No Hit
CATTTAAACCCTACTAAGACTAAACGTTGGGGCTACTCCCTTAACTTCAT8870.1388230249020648No Hit
CCTTTGCAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT8630.13506682129704842No Hit
GTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC8540.13365824494516726No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8530.13350173646162491No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG8460.13240617707682845No Hit
CTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTA8270.12943251588952379No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC8120.12708488863638853No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC8020.12551980380096503No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAA7740.12113756626177923No Hit
GTCTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG7590.11878993900864396No Hit
ATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGAAACATAGAGGTTC7530.11785088810738985No Hit
CTTATAAGACCTTTCTGTGTAAACAATACCTGAACCTTTACCCCGTTGCT7520.1176943796238475No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG7490.11722485417322046No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC7470.11691183720613577No Hit
CCCTACTAAGACTAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG7360.11519024388716989No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7250.11346865056820404No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC7140.1117470572492382No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA7100.11112102331506879No Hit
ATTGATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT6930.10846037909484883No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG6820.10673878577588299No Hit
GACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG6780.10611275184171359No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC6750.10564322639108653No Hit
GACGCAAATTGCACTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAG6740.10548671790754419No Hit
ATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAA6710.10501719245691714No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG6660.10423465003920537No Hit
CTTCATCTGCCGTTCCGGCCGACCACGGGGCGCACCTCTCTTTACGCGGT6660.10423465003920537No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6620.10360861610503598No Hit
CTCTTATATAAAATACCAGCCTTCCATAGAGTATGTAAATAATGCCTAGT6590.10313909065440892No Hit
TTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAA6480.10141749733544307No Hit
ATCCGGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATTTACAT6470.10126098885190071No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)