FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences602050
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33560.5574287849846358No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT20760.34482185864961384No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG20630.34266256955402374No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG19960.3315339257536749No Hit
CCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA18940.3145918113113529No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG18520.30761564654098494No Hit
ATTTATAGGAAGTTTCCTAAAACATTGTTTGATTTTGTGTACAATATGTT16660.2767212025579271No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG15320.2544639149572295No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT13800.2292168424549456No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT13190.21908479362179223No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT12660.21028153807823272No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC11910.19782410098829No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA11900.19765800182709076No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA11370.18885474628353127No Hit
AGTTCAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA10780.17905489577277636No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA10430.1732414251308031No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10140.16842454945602525No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG10110.16792625197242755No Hit
CCCTGAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG10040.16676355784403288No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAA9600.15945519475126652No Hit
AGTCTGTACAACATCTTGAATCCCTTTATACCGCTATTACCAATTTTCTT9410.15629931068848102No Hit
CAATAGGCCTATTTATAGGAAGTTTCCTAAAACATTGTTTGATTTTGTGT9350.1553027157212856No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT8890.14766215430612076No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA8870.14732995598372228No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA8740.1451706668881322No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC8700.1445062702433353No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC8460.14051989037455362No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG8300.13786230379536582No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG8280.13753010547296737No Hit
AAAACTGATTATGCCTGCTAGGTTCTATCCTAACCGTACCAAATATTTGC7820.1298895440578025No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG7790.12939124657420478No Hit
ATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAA7750.12872684992940786No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC7740.12856075076820864No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC7690.12773025496221244No Hit
CTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA7500.12457437089942697No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA7480.12424217257702849No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC7450.12374387509343078No Hit
GGCCTATTTATAGGAAGTTTCCTAAAACATTGTTTGATTTTGTGTACAAT7410.12307947844863384No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGA7270.12075409019184453No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7150.1187609002574537No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6900.1146084212274728No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6890.11444232206627356No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG6820.11327962793787891No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6740.11195083464828502No Hit
CCTATAAATAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT6740.11195083464828502No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6740.11195083464828502No Hit
GCCTAGCAGCCATGGAAATGAGGTGTATTTCCGAGAGAGGACAACAGAGT6680.1109542396810896No Hit
CCTTTTTACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6650.1104559421974919No Hit
GCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTA6630.11012374387509344No Hit
GTATAAAGGGATTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC6620.10995764471389419No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6590.10945934723029647No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC6470.10746615729590565No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6430.10680176065110872No Hit
TTGACATACTTTCCAATCAATAGGCCTATTTATAGGAAGTTTCCTAAAAC6380.10597126484511252No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6200.10298147994352627No Hit
GTATGTAAATAATGCCTGGTTTGGAAGTAATGATTAACTATGTGTTCAGG6200.10298147994352627No Hit
ATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGG6110.10148658749273315No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA6050.10048999252553774No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTATAAA6040.10032389336433852No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)