FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences602050
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33450.5556016942114442No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG24230.40245826758574865No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT19010.31575450543974753No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG18620.30927663815297735No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG17490.29050743293746367No Hit
CCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA17080.283697367328295No Hit
ATTTATAGGAAGTTTCCTAAAACATTGTTTGATTTTGTGTACAATATGTT16620.27605680591313014No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG15650.2599451872768042No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT13440.22323727265177312No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT13150.21842039697699528No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC12850.21343742214101818No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT12500.20762395149904492No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA11470.19051573789552362No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG11090.18420396976995265No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA11030.18320737480275726No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10080.16742795448882986No Hit
CCCTGAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG9650.1602856905572627No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA9420.15646540984968027No Hit
AGTTCAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA9360.15546881488248485No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA9230.15330952578689477No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG9020.14982144340171083No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA8960.1488248484345154No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC8850.1469977576613238No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAA8800.14616726185532763No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC8450.14035379121335437No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT8090.13437422141018188No Hit
AGTCTGTACAACATCTTGAATCCCTTTATACCGCTATTACCAATTTTCTT8030.13337762644298645No Hit
GGCCTATTTATAGGAAGTTTCCTAAAACATTGTTTGATTTTGTGTACAAT7930.1317166348309941No Hit
CAATAGGCCTATTTATAGGAAGTTTCCTAAAACATTGTTTGATTTTGTGT7910.13138443650859563No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG7660.12723195747861474No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG7610.12640146167261854No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC7490.12440827173822772No Hit
CTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA7450.12374387509343078No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGA7380.12258118096503613No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC7350.12208288348143842No Hit
GTATAAAGGGATTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC7280.12092018935304376No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC7260.1205879910306453No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC7220.11992359438584835No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7140.11859480109625448No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG7100.11793040445145751No Hit
GTATGTAAATAATGCCTGGTTTGGAAGTAATGATTAACTATGTGTTCAGG7060.11726600780666058No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA6970.11577111535586745No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6810.11311352877667968No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6780.11261523129308197No Hit
AAAACTGATTATGCCTGCTAGGTTCTATCCTAACCGTACCAAATATTTGC6760.1122830329706835No Hit
GCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTA6700.11128643800348809No Hit
ATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAA6680.1109542396810896No Hit
CCTATAAATAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT6590.10945934723029647No Hit
CCTTTTTACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6490.10779835561830413No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACCG6450.10713395897350719No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6430.10680176065110872No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6420.10663566148990948No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6290.1044763723943194No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6280.10431027323312017No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6220.10331367826592476No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTATACC6200.10298147994352627No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6200.10298147994352627No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6200.10298147994352627No Hit
GCCTAGCAGCCATGGAAATGAGGTGTATTTCCGAGAGAGGACAACAGAGT6090.1011543891703347No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTATAAA6080.10098829000913545No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA6050.10048999252553774No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)