FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525535
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTATAGGAAGTTTCCGAAAGCATTGTTTGATTTTATGTACAATATGTT75551.4375826538670116No Hit
CATTTAAACCCTGCTAAAACTAAACGTTGGGGCTACTCCCTTCACTTCAT57911.1019247053003132No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT38080.724594936588429No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA34740.661040653809927No Hit
TTATAGGAAGTTTCCGAAAGCATTGTTTGATTTTATGTACAATATGTTCC33660.6404901671629863No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC30500.5803609654923079No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT28500.5423045087387138No Hit
CCCACGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACT28130.535264064239299No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA27460.5225151512268451No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTGAAGGGAG23450.44621195543588915No Hit
GGGTAAAGGTTCAAATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA22040.4193821534246054No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC21810.41500566089794205No Hit
GTTTCCGAAAGCATTGTTTGATTTTATGTACAATATGTTCCTGTGGTAAG21720.4132931203440304No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21110.4016859010341842No Hit
TATAGGAAGTTTCCGAAAGCATTGTTTGATTTTATGTACAATATGTTCCT21110.4016859010341842No Hit
CTAGCAAACTGAGCCATGAGAAACGGACTGAGGCCCACTCCCATAGGAAT21050.40054420733157636No Hit
CTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTG20010.38075484981970753No Hit
ATGTGGTATTGGGGGCCAAGACTGTACAACATCTTGAATCCCTTTTTACC18100.34441093362002534No Hit
ATTTATGGGAAGTTTCCGAAAGCATTGTTTGATTTTATGTACAATATGTT18040.3432692399174175No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGTCAACGGAACCTCTATG17770.33813161825568233No Hit
AGACTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT17660.3360385131342346No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCATG17420.3314717383238034No Hit
TTTCAGTTATGTGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATC17200.32728552808090805No Hit
CCCACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACT17100.3253827052432283No Hit
TAACCGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAAT16940.32233818870294084No Hit
GCCCACTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAAT16810.3198645190139572No Hit
GAGTAGCCCCAACGTTTAGTTTTAGCAGGGTTTAAATGTATACCCAAAGA16740.31853254302758144No Hit
TCCCATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGGC16600.31586859105482984No Hit
GTACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCC16420.31244350994700637No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC16210.308447581987879No Hit
CTAGCAAACTGAGCCATGAGAAACGGACTGAGGCCCATTCCCATAGGAAT15600.2968403626780329No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGCGTAAAG15510.29512782212412114No Hit
TTTCAGTTATGTGGATGATGTGGTATTGGGGGCCAAGACTGTACAACATC14890.283330320530507No Hit
GGCCAAGACTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATT14870.2829497559629711No Hit
CAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTC14860.2827594736792031No Hit
CCCTCGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTGTGGTATTG14660.2789538280038437No Hit
TTTCTAGGGGGAGCACCCATGTGTCCTGGCCAAAATTCGCAGTCCCCAAC14020.26677576184269364No Hit
TGCAAGACCTGCACGACTCCTGTCAACGGAACCTCTATGTTTCCCTCTTG13850.26354096301863816No Hit
CTTCCCATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGG13700.2606867287621186No Hit
CTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAGGAACATA13390.25478797796531155No Hit
ACACATGGGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA12990.24717668661459274No Hit
GTTTGCTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT12550.23880426612880207No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCTTTGACATACT12080.22986099879170752No Hit
GGGCTACTCCCTTCACTTCATGGGATATGTAATTGGAAGTTGGGGTACCT11800.22453309484620435No Hit
CAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATG11740.2233914011435965No Hit
ATGTAATTGGAAGTTGGGGTACCTTACCACAGGAACATATTGTACATAAA11620.2211080137383809No Hit
TTTAAACCCTGCTAAAACTAAACGTTGGGGCTACTCCCTTCACTTCATGG11480.2184440617656293No Hit
TTTCTAGGGGGAGCACCCACGTGTCCTGGCCAAAATTTGCAGTCCCCAAC11460.21806349719809334No Hit
GGAATGGGCCTCAGTCCGTTTCTCATGGCTCAGTTTGCTAGTGCCATTTG11460.21806349719809334No Hit
ATACCACATCATCCACATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA11420.2173023680630215No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC11390.21673152121171757No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCC11300.21501898065780586No Hit
GGATATGTAATTGGAAGTTGGGGTACCTTACCACAGGAACATATTGTACA11300.21501898065780586No Hit
GCGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCTTTGACATACT11120.2115938995499824No Hit
GTTTGATTTTATGTACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCA11090.21102305269867846No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC11040.21007164127983866No Hit
CTAGTAAACTGAGCCATGAGAAACGGACTGAGGCCCACTCCCATAGGAAT10790.2053145841856394No Hit
CCCATGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACT10490.1996061156726003No Hit
AATTCGCAGTCCCCAACCTCCAATCACTCACTAACCTCTTGTCCTCCAAT10200.19408792944332917No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG10180.19370736487579324No Hit
TATGGGAAGTTTCCGAAAGCATTGTTTGATTTTATGTACAATATGTTCCT10170.19351708259202527No Hit
TTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTATACAA10160.1933268003082573No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG10070.19161425975434557No Hit
CCCATGAAGTGAAGGGAGTAGCCCCAACGTTTAGTTTTAGCAGGGTTTAA10000.1902822837679698No Hit
GTATTGGGGGCCAAGACTGTACAACATCTTGAATCCCTTTTTACCGCTGT9980.18990171920043383No Hit
GGTCTATTTATAGGAAGTTTCCGAAAGCATTGTTTGATTTTATGTACAAT9960.1895211546328979No Hit
CCTTACCACAGGAACATATTGTACATAAAATCAAACAATGCTTTCGGAAA9870.18780861407898616No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCGAGGGGGAGCACCCACG9580.18229042784971505No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC9560.18190986328217912No Hit
GTCTTTGGGTATACATTTAAACCCTGCTAAAACTAAACGTTGGGGCTACT9510.18095845186333925No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC9460.18000704044449942No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT9370.17829449989058768No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG9260.17620139476914No Hit
GTTCAAATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGAGAAAGTGA9250.17601111248537205No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAGCATTGTTTGATTTTATGT9230.1756305479178361No Hit
AAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCT9170.17448885421522828No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTATAGGAAGTTTCCGAAAGC9050.17220546681001267No Hit
GTGAAGGGAGTAGCCCCAACGTTTAGTTTTAGCAGGGTTTAAATGTATAC9040.1720151845262447No Hit
GAATACAAGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA8980.17087349082363687No Hit
GGGAATGGGCCTCAGTCCGTTTCTCATGGCTCAGTTTGCTAGTGCCATTT8980.17087349082363687No Hit
GCCCAGAAGCCCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT8910.16954151483726107No Hit
GCCTATGGCCAAGCCCCAACCAGTGGGAGTTGCGTCAGCAAACACTTGGC8880.16897066798595717No Hit
GGAACATATTGTACATAAAATCAAACAATGCTTTCGGAAACTTCCTATAA8870.1687803857021892No Hit
GGGTATACATTTAAACCCTGCTAAAACTAAACGTTGGGGCTACTCCCTTC8830.1680192565671173No Hit
TACATATCCCATGAAGTGAAGGGAGTAGCCCCAACGTTTAGTTTTAGCAG8820.16782897428334934No Hit
CTCCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACCGAAGATG8800.1674484097158134No Hit
GCCCCAACCAGTGGGAGTTGCGTCAGCAAACACTTGGCAAAGACCTGACC8660.16478445774306183No Hit
CCCTTTACGCAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT8650.16459417545929386No Hit
ACACATGGGTGCTCCCCCTCGAAAATTGAGAGAAGTCCACCACGAGTCTA8610.163833046324222No Hit
ACATAGAGGTTCCGTTGACAGGAGTCGTGCAGGTCTTGCATGGTCCCGTG8600.16364276404045403No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATGTGGATGATGTGGTATTGGGGGC8470.16116909435147042No Hit
ATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT8450.16078852978393446No Hit
GGTTCAAATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGAGAAAGTG8430.16040796521639852No Hit
CCTTATAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGCTTGTATACATGCA8280.15755373095987898No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGGCT8270.15736344867611102No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA8250.15698288410857505No Hit
GGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAA8080.15374808528451958No Hit
ATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGT7950.15127441559553598No Hit
CAATTACATATCCCATGAAGTGAAGGGAGTAGCCCCAACGTTTAGTTTTA7850.14937159275785628No Hit
GTACAAAACCTTCGGACGGAAATTGCACTTGTATTCCCATCCCATCATCT7830.14899102819032034No Hit
CATTGCGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCTTTGACA7730.14708820535264064No Hit
GTCTTGGCCCCCAATACCACATCATCCACATAACTGAAAGCCAAACAGTG7730.14708820535264064No Hit
CCCTTCACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG7730.14708820535264064No Hit
CCCACTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATA7700.14651735850133674No Hit
GTGGTATTGGGGGCCAAGACTGTACAACATCTTGAATCCCTTTTTACCGC7600.14461453566365703No Hit
ACCTCCAATCACTCACTAACCTCTTGTCCTCCAATTTGTCCTGGTTATCG7580.14423397109612107No Hit
CAGAAAGGCCTTATAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGCTTGTA7520.1430922773935133No Hit
AGTGAAGGGAGTAGCCCCAACGTTTAGTTTTAGCAGGGTTTAAATGTATA7510.1429019951097453No Hit
GCCTTATAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGCTTGTATACATGC7450.1417603014071375No Hit
CGTTTAGTTTTAGCAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATT7410.1409991722720656No Hit
GTTTTAGCAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAAC7410.1409991722720656No Hit
AACTAAACGTTGGGGCTACTCCCTTCACTTCATGGGATATGTAATTGGAA7390.14061860770452966No Hit
GAAGTGAAGGGAGTAGCCCCAACGTTTAGTTTTAGCAGGGTTTAAATGTA7290.13871578486685No Hit
ATGTTGTACAGTCTTGGCCCCCAATACCACATCATCCACATAACTGAAAG7260.13814493801554606No Hit
GGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATT7230.13757409116424216No Hit
ATGCAAGACCTGCACGACTCCTGTCAACGGAACCTCTATGTTTCCCTCTT7170.13643239746163432No Hit
GGTATACATTTAAACCCTGCTAAAACTAAACGTTGGGGCTACTCCCTTCA7150.13605183289409842No Hit
CTTCACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAGGA7080.13471985690772262No Hit
AAGATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA7010.13338788092134682No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT6930.13186562265120305No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTACCAAGGTATGTTGC6920.13167534036743508No Hit
CTGATGGCCTATGGCCAAGCCCCAACCAGTGGGAGTTGCGTCAGCAAACA6900.13129477579989915No Hit
CCCATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGGCT6890.13110449351613118No Hit
GACCTGACCGTTGCCGAGCAACGGGGTAAAGGTTCAAATATTGTTTACAC6850.1303433643810593No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC6820.12977251752975538No Hit
GGACCATGCAAGACCTGCACGACTCCTGTCAACGGAACCTCTATGTTTCC6800.12939195296221945No Hit
ACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCGAGGACT6640.12634743642193194No Hit
CCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGATTCAAGATG6620.125966871854396No Hit
TTGTTTACACAGAAAGGCCTTATAAGTTGGCGAGAAAGTGAAAGCCTGCT6560.12482517815178817No Hit
GTACAGTCTTGGCCCCCAATACCACATCATCCACATAACTGAAAGCCAAA6430.12235150846280458No Hit
GGGTAACCACATTGCGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAAT6390.1215903793277327No Hit
GCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGG6270.11930699192251705No Hit
ATACCACATCATCCACATAACCGAAAGCCAAACAGTGGGGGAAAGCCCTA6170.11740416908483735No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA6100.11607219309846158No Hit
ATGTACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATC6100.11607219309846158No Hit
CTCACTAACCTCTTGTCCTCCAATTTGTCCTGGTTATCGTTGGATGTGTC6040.11493049939585376No Hit
GGAATGGGCCTCAGTCCGTTTCTCATGGCTCAGTTTACTAGTGCCATTTG6010.11435965254454983No Hit
TGTTTACACAGAAAGGCCTTATAAGTTGGCGAGAAAGTGAAAGCCTGCTT5890.1120762651393342No Hit
TCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTAC5860.1115054182880303No Hit
CACTAACCTCTTGTCCTCCAATTTGTCCTGGTTATCGTTGGATGTGTCTG5780.10998316001788652No Hit
CGCATGCGCTGATGGCCTATGGCCAAGCCCCAACCAGTGGGAGTTGCGTC5720.10884146631527872No Hit
GGTTCCACGCATGCGCTGATGGCCTATGGCCAAGCCCCAACCAGTGGGAG5710.10865118403151075No Hit
GGATGAAGAGGAATATGATAAAACGCCGCAGACACATCCAACGATAACCA5700.10846090174774278No Hit
AAATTCGCAGTCCCCAACCTCCAATCACTCACTAACCTCTTGTCCTCCAA5700.10846090174774278No Hit
GCCCAAGATGATGGGATGGGAATACAAGTGCAATTTCCGTCCGAAGGTTT5660.1076997726126709No Hit
GCCCCAACGTTTAGTTTTAGCAGGGTTTAAATGTATACCCAAAGACAAAA5650.10750949032890293No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGT5580.10617751434252715No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG5510.10484553835615135No Hit
CCTCCAATCACTCACTAACCTCTTGTCCTCCAATTTGTCCTGGTTATCGT5500.10465525607238338No Hit
CAAATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGAGAAAGTGAAAG5460.1038941269373115No Hit
ACTCCTGTCAACGGAACCTCTATGTTTCCCTCTTGTTGCTGTACAAAACC5460.1038941269373115No Hit
CTCCCTTCACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCAC5450.10370384465354353No Hit
ACTCCCTTCACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCA5430.1033232800860076No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5410.10294271551847164No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG5410.10294271551847164No Hit
GACCTGCACGACTCCTGTCAACGGAACCTCTATGTTTCCCTCTTGTTGCT5370.10218158638339978No Hit
CATGCATACAAAGGCATTAATGCTGGGTAACCACATTGCGTAAAGGGAGC5360.1019913040996318No Hit
GCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCCTATGGCCAAG5320.10123017496455992No Hit
GCTTGTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGCGT5280.10046904582948805No Hit
GTGGTTACCCAGCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAG5280.10046904582948805No Hit
GCTAAAACTAAACGTTGGGGCTACTCCCTTCACTTCATGGGATATGTAAT5260.1000884812619521No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)