FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525535
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTATAGGAAGTTTCCGAAAGCATTGTTTGATTTTATGTACAATATGTT76431.4543274948385931No Hit
CATTTAAACCCTGCTAAAACTAAACGTTGGGGCTACTCCCTTCACTTCAT55551.057018086331072No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA39880.7588457476666635No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT39770.7567526425452159No Hit
TTATAGGAAGTTTCCGAAAGCATTGTTTGATTTTATGTACAATATGTTCC32470.6178465753945979No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC31890.6068102029360556No Hit
GTTTCCGAAAGCATTGTTTGATTTTATGTACAATATGTTCCTGTGGTAAG30390.5782678603708602No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA27080.5152844244436622No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT26400.5023452291474402No Hit
GGGTAAAGGTTCAAATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA23190.44126461605792194No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTGAAGGGAG21010.3997830781965045No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20940.39845110221012875No Hit
CTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTG20070.3818965435223154No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCATG19770.37618807500927626No Hit
TATAGGAAGTTTCCGAAAGCATTGTTTGATTTTATGTACAATATGTTCCT19530.371621300198845No Hit
ATTTATGGGAAGTTTCCGAAAGCATTGTTTGATTTTATGTACAATATGTT19110.36362944428059024No Hit
ATGTGGTATTGGGGGCCAAGACTGTACAACATCTTGAATCCCTTTTTACC19030.3621071860104465No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC18890.3594432340376949No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGTCAACGGAACCTCTATG17910.3407955702284339No Hit
GTACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCC17800.3387024651069862No Hit
AGACTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT17050.3244312938243885No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC16800.31967423673018924No Hit
TCCCATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGGC16690.3175811316087416No Hit
CTAGCAAACTGAGCCATGAGAAACGGACTGAGGCCCACTCCCATAGGAAT16430.31263379223077437No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGCGTAAAG16390.3118726630957025No Hit
TTTCAGTTATGTGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATC16310.3103504048255587No Hit
TTTCAGTTATGTGGATGATGTGGTATTGGGGGCCAAGACTGTACAACATC15660.2979820563806407No Hit
TTTCTAGGGGGAGCACCCATGTGTCCTGGCCAAAATTCGCAGTCCCCAAC14970.28485257880065074No Hit
TAACCGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAAT14940.28428173194934686No Hit
GAGTAGCCCCAACGTTTAGTTTTAGCAGGGTTTAAATGTATACCCAAAGA14660.2789538280038437No Hit
CTAGCAAACTGAGCCATGAGAAACGGACTGAGGCCCATTCCCATAGGAAT14520.2762898760310921No Hit
GGCCAAGACTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATT14380.2736259240583406No Hit
GCCCACTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAAT14220.27058140751805304No Hit
GTTTGCTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT14060.2675368909777655No Hit
CCCTCGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTGTGGTATTG13570.25821305907313497No Hit
CTTCCCATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGG13240.251933743708792No Hit
CTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAGGAACATA13080.24888922716850445No Hit
CAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTC12840.2443224523580732No Hit
TGCAAGACCTGCACGACTCCTGTCAACGGAACCTCTATGTTTCCCTCTTG12760.24280019408792947No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCTTTGACATACT12620.24013624211517787No Hit
GTTTGATTTTATGTACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCA12340.2348083381696747No Hit
ATGTAATTGGAAGTTGGGGTACCTTACCACAGGAACATATTGTACATAAA12140.23100269249431532No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG11350.21597039207664573No Hit
GGGCTACTCCCTTCACTTCATGGGATATGTAATTGGAAGTTGGGGTACCT11210.21330644010389413No Hit
GGAATGGGCCTCAGTCCGTTTCTCATGGCTCAGTTTGCTAGTGCCATTTG11150.21216474640128632No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC11070.21064248813114253No Hit
GGTCTATTTATAGGAAGTTTCCGAAAGCATTGTTTGATTTTATGTACAAT11050.21026192356360662No Hit
CAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATG11000.20931051214476676No Hit
TTTAAACCCTGCTAAAACTAAACGTTGGGGCTACTCCCTTCACTTCATGG10990.20912022986099876No Hit
TTTCTAGGGGGAGCACCCACGTGTCCTGGCCAAAATTTGCAGTCCCCAAC10880.2070271247395511No Hit
GTTCAAATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGAGAAAGTGA10790.2053145841856394No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC10760.20474373733433548No Hit
ACACATGGGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA10670.20303119678042378No Hit
GCGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCTTTGACATACT10400.1978935751186886No Hit
GTCTTTGGGTATACATTTAAACCCTGCTAAAACTAAACGTTGGGGCTACT10370.19732272826738467No Hit
CCCATGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACT10360.1971324459836167No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCGAGGGGGAGCACCCACG10330.19656159913231278No Hit
GTATTGGGGGCCAAGACTGTACAACATCTTGAATCCCTTTTTACCGCTGT10210.19427821172709714No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC10150.19313651802448933No Hit
GGATATGTAATTGGAAGTTGGGGTACCTTACCACAGGAACATATTGTACA10150.19313651802448933No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCC10070.19161425975434557No Hit
ATACCACATCATCCACATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA10020.19066284833550573No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG9890.18818917864652213No Hit
AATTCGCAGTCCCCAACCTCCAATCACTCACTAACCTCTTGTCCTCCAAT9760.18571550895753852No Hit
CTAGTAAACTGAGCCATGAGAAACGGACTGAGGCCCACTCCCATAGGAAT9570.18210014556594709No Hit
GCCCAGAAGCCCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT9560.18190986328217912No Hit
GGTTCAAATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGAGAAAGTG9560.18190986328217912No Hit
GCCTATGGCCAAGCCCCAACCAGTGGGAGTTGCGTCAGCAAACACTTGGC9420.17924591130942755No Hit
CCTTACCACAGGAACATATTGTACATAAAATCAAACAATGCTTTCGGAAA9390.17867506445812362No Hit
CTCCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACCGAAGATG9370.17829449989058768No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG9320.17734308847174785No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT9140.17391800736392438No Hit
TTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTATACAA9070.1725860313775486No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAGCATTGTTTGATTTTATGT9060.1723957490937806No Hit
CCTTATAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGCTTGTATACATGCA9040.1720151845262447No Hit
TATGGGAAGTTTCCGAAAGCATTGTTTGATTTTATGTACAATATGTTCCT8990.17106377310740484No Hit
GTACAAAACCTTCGGACGGAAATTGCACTTGTATTCCCATCCCATCATCT8800.1674484097158134No Hit
GCCCCAACCAGTGGGAGTTGCGTCAGCAAACACTTGGCAAAGACCTGACC8780.16706784514827747No Hit
CCCTTCACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG8760.16668728058074153No Hit
GGAACATATTGTACATAAAATCAAACAATGCTTTCGGAAACTTCCTATAA8750.16649699829697356No Hit
CCCATGAAGTGAAGGGAGTAGCCCCAACGTTTAGTTTTAGCAGGGTTTAA8740.1663067160132056No Hit
GGGTATACATTTAAACCCTGCTAAAACTAAACGTTGGGGCTACTCCCTTC8690.16535530459436573No Hit
CCCTTTACGCAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT8540.1625010703378462No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATGTGGATGATGTGGTATTGGGGGC8520.16212050577031026No Hit
GAATACAAGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA8490.16154965891900633No Hit
ACATAGAGGTTCCGTTGACAGGAGTCGTGCAGGTCTTGCATGGTCCCGTG8480.1613593766352384No Hit
ATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGT8440.1605982475001665No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC8400.15983711836509462No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGGCT8340.1586954246624868No Hit
ATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT8320.15831486009495085No Hit
ATGTTGTACAGTCTTGGCCCCCAATACCACATCATCCACATAACTGAAAG8310.1581245778111829No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTATAGGAAGTTTCCGAAAGC8200.15603147268973522No Hit
TACATATCCCATGAAGTGAAGGGAGTAGCCCCAACGTTTAGTTTTAGCAG8170.15546062583843132No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA8140.1548897789871274No Hit
GTCTTGGCCCCCAATACCACATCATCCACATAACTGAAAGCCAAACAGTG8130.15469949670335945No Hit
GTGAAGGGAGTAGCCCCAACGTTTAGTTTTAGCAGGGTTTAAATGTATAC8100.15412864985205554No Hit
GTTTTAGCAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAAC8050.15317723843321568No Hit
GGGAATGGGCCTCAGTCCGTTTCTCATGGCTCAGTTTGCTAGTGCCATTT8030.15279667386567974No Hit
GGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAA7850.14937159275785628No Hit
CCCACGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACT7820.14880074590655237No Hit
AAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCT7810.1486104636227844No Hit
ACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCGAGGACT7730.14708820535264064No Hit
GCCTTATAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGCTTGTATACATGC7650.14556594708249687No Hit
CTTCACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAGGA7600.14461453566365703No Hit
CAATTACATATCCCATGAAGTGAAGGGAGTAGCCCCAACGTTTAGTTTTA7580.14423397109612107No Hit
CGTTTAGTTTTAGCAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATT7450.1417603014071375No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA7340.13966719628568983No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC7330.13947691400192186No Hit
AACTAAACGTTGGGGCTACTCCCTTCACTTCATGGGATATGTAATTGGAA7230.13757409116424216No Hit
GGTATACATTTAAACCCTGCTAAAACTAAACGTTGGGGCTACTCCCTTCA7210.1371935265967062No Hit
ATGCAAGACCTGCACGACTCCTGTCAACGGAACCTCTATGTTTCCCTCTT7210.1371935265967062No Hit
CATTGCGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCTTTGACA7050.13414901005641872No Hit
CTCCCTTCACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCAC7030.13376844548888275No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT6960.13243646950250698No Hit
GACCTGACCGTTGCCGAGCAACGGGGTAAAGGTTCAAATATTGTTTACAC6860.13053364666482728No Hit
ACCTCCAATCACTCACTAACCTCTTGTCCTCCAATTTGTCCTGGTTATCG6820.12977251752975538No Hit
GGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATT6780.1290113883946835No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTACCAAGGTATGTTGC6700.12748913012453975No Hit
AGTGAAGGGAGTAGCCCCAACGTTTAGTTTTAGCAGGGTTTAAATGTATA6640.12634743642193194No Hit
GGGTAACCACATTGCGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAAT6620.125966871854396No Hit
CACTAACCTCTTGTCCTCCAATTTGTCCTGGTTATCGTTGGATGTGTCTG6610.12577658957062804No Hit
CTCACTAACCTCTTGTCCTCCAATTTGTCCTGGTTATCGTTGGATGTGTC6570.12501546043555614No Hit
GTGGTATTGGGGGCCAAGACTGTACAACATCTTGAATCCCTTTTTACCGC6560.12482517815178817No Hit
TTGTTTACACAGAAAGGCCTTATAAGTTGGCGAGAAAGTGAAAGCCTGCT6510.12387376673294832No Hit
GGACCATGCAAGACCTGCACGACTCCTGTCAACGGAACCTCTATGTTTCC6500.12368348444918036No Hit
CCCATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGGCT6480.12330291988164443No Hit
CAGAAAGGCCTTATAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGCTTGTA6410.12197094389526864No Hit
GTACAGTCTTGGCCCCCAATACCACATCATCCACATAACTGAAAGCCAAA6280.11949727420628503No Hit
GTCCCCAACCTCCAATCACTCACTAACCTCTTGTCCTCCAATTTGTCCTG6250.1189264273549811No Hit
GAAGTGAAGGGAGTAGCCCCAACGTTTAGTTTTAGCAGGGTTTAAATGTA6240.11873614507121315No Hit
CCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGATTCAAGATG6190.1177847336523733No Hit
AAGATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA6150.11702360451730143No Hit
TCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTAC6100.11607219309846158No Hit
CTGATGGCCTATGGCCAAGCCCCAACCAGTGGGAGTTGCGTCAGCAAACA6070.11550134624715766No Hit
GCCCAAGATGATGGGATGGGAATACAAGTGCAATTTCCGTCCGAAGGTTT6010.11435965254454983No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGT5950.11321795884194202No Hit
AAATTCGCAGTCCCCAACCTCCAATCACTCACTAACCTCTTGTCCTCCAA5830.11093457143672637No Hit
GCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGG5810.11055400686919045No Hit
GGATGAAGAGGAATATGATAAAACGCCGCAGACACATCCAACGATAACCA5800.11036372458542247No Hit
CTTTCCAATCAATAGGTCTATTTATAGGAAGTTTCCGAAAGCATTGTTTG5780.10998316001788652No Hit
TGTTTACACAGAAAGGCCTTATAAGTTGGCGAGAAAGTGAAAGCCTGCTT5780.10998316001788652No Hit
GAATATGATAAAACGCCGCAGACACATCCAACGATAACCAGGACAAATTG5770.10979287773411857No Hit
GTTCCGGAACAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCA5720.10884146631527872No Hit
ATGTACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATC5710.10865118403151075No Hit
CCTCCAATCACTCACTAACCTCTTGTCCTCCAATTTGTCCTGGTTATCGT5700.10846090174774278No Hit
ACTCCTGTCAACGGAACCTCTATGTTTCCCTCTTGTTGCTGTACAAAACC5680.10808033718020682No Hit
ACACATGGGTGCTCCCCCTCGAAAATTGAGAGAAGTCCACCACGAGTCTA5670.10789005489643888No Hit
GGTTATCGTTGGATGTGTCTGCGGCGTTTTATCATATTCCTCTTCATCCT5620.10693864347759903No Hit
GGTTCCACGCATGCGCTGATGGCCTATGGCCAAGCCCCAACCAGTGGGAG5590.1063677966262951No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGGCTTCTGG5560.1057969497749912No Hit
ACTCCCTTCACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCA5550.10560666749122323No Hit
GTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCGAGGACTGGGGA5540.10541638520745525No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGCAAACTGAG5490.1044649737886154No Hit
GCTTGTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGCGT5420.10313299780223963No Hit
CCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACCGAAGATGGA5350.10180102181586383No Hit
ATACCACATCATCCACATAACCGAAAGCCAAACAGTGGGGGAAAGCCCTA5340.10161073953209587No Hit
GCCCCAACGTTTAGTTTTAGCAGGGTTTAAATGTATACCCAAAGACAAAA5320.10123017496455992No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)