FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345722
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13030.37689241645021143No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG12520.36214067950549866No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT11550.3340834543361429No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATCATGCCTGCTAGG9310.26929151167701215No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9110.26350651679673265No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAT7780.22503630084287374No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT7690.22243305314674794No Hit
GGTTAGGATAGAACCTAGCAGGCATGATTAATTTTAATCTCCTTTTTTCA7130.2062350674819653No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG7040.2036318197858395No Hit
GTGTAAAGGGAGCGGCAAACCCCAAAAGACCCACAATTCTTTGACATACT6740.19495432746542019No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6310.1825165884728192No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6110.17673159359253968No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT6100.1764423438485257No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5430.15706261099958926No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5320.15388086381543553No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA5290.1530131145833936No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5260.15214536535135165No Hit
GTCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG5230.15127761611930973No Hit
GCATCAAACCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCT5220.15098836637529575No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTT5060.14636037047107214No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT4990.1443356222629743No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT4920.14231087405487647No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT4880.14115387507882057No Hit
CATTAAGACTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT4870.14086462533480656No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATAACCCATGAA4810.13912912687072274No Hit
CTCTTGGGGTGGAGCCCTCAGGCTCAGGGCATATTGACAACAGTACCAGC4790.13855062738269477No Hit
CATTGAGACTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT4780.1382613776386808No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC4770.1379721278946668No Hit
CCTCCCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4690.135658129942555No Hit
CTCTTAGGGTGGAGCCCTCAGGCTCAGGGCATATTGACAACAGTACCAGC4670.13507963045452706No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA4660.13479038071051308No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTCAACTTCAT4640.13421188122248512No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA4640.13421188122248512No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC4620.13363338173445716No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4600.13305488224642922No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG4600.13305488224642922No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4590.13276563250241524No Hit
GGATAGAACCTAGCAGGCATGATTAATTTTAATCTCCTTTTTTCATTAAC4560.1318978832703733No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAAC4520.1307408842943174No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAT4520.1307408842943174No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT4510.13045163455030342No Hit
CCCCAAGAGATTGCCGTGTGGTGGAGTGAACCCCGGCCCGAATGATCCCA4460.12900538583023355No Hit
CCTACCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4450.12871613608621957No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4410.12755913711016365No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTCGGAAACTTCCTATAA4360.12611288839009377No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4350.12582363864607982No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4330.12524513915805183No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4300.12437738992600993No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4300.12437738992600993No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTT4220.1220633919738981No Hit
CAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGA4120.11917089453375834No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA4060.11743539606967447No Hit
ATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTTAG3970.11483214837354869No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG3920.1133858996534788No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG3910.11309664990946484No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC3860.11165040118939495No Hit
GTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG3810.11020415246932506No Hit
GCTTACTACTAATTCCCTGGATGCCGGGTCTTCCAAATTACTTCCCACCC3710.1073116550291853No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC3680.10644390579714338No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3580.1035514083570036No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC3540.10239440938094771No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCA3470.10036966117284987No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3460.10008041142883588No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATCATGCCTGCTAGGTT3460.10008041142883588No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)