FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345722
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG15080.4361886139730767No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12950.37457841849809964No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATCATGCCTGCTAGG9370.27102701014109604No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9020.26090326910060685No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT8780.2539612752442714No Hit
GGTTAGGATAGAACCTAGCAGGCATGATTAATTTTAATCTCCTTTTTTCA8070.23342454341927907No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAT7880.22792879828301352No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7670.22185455365872No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT7550.21838355673055226No Hit
GTGTAAAGGGAGCGGCAAACCCCAAAAGACCCACAATTCTTTGACATACT7460.2157803090344265No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG6800.19668982592950407No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6660.1926403295133084No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6500.18801233360908476No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT6280.18164883924077727No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA6120.17702084333655366No Hit
GTCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG5970.172682097176344No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5660.16371535511191074No Hit
CCCCAAGAGATTGCCGTGTGGTGGAGTGAACCCCGGCCCGAATGATCCCA5480.15850885971965917No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT5410.15648411151156133No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT5300.15330236432740757No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC5220.15098836637529575No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5210.15069911663128177No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC5090.14722811970311409No Hit
CTCTTGGGGTGGAGCCCTCAGGCTCAGGGCATATTGACAACAGTACCAGC5030.1454926212390302No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATAACCCATGAA5000.14462487200698826No Hit
GTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG4990.1443356222629743No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT4980.14404637251896033No Hit
CCTCCCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4910.1420216243108625No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4790.13855062738269477No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4760.13768287815065283No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG4760.13768287815065283No Hit
CTCTTAGGGTGGAGCCCTCAGGCTCAGGGCATATTGACAACAGTACCAGC4660.13479038071051308No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA4640.13421188122248512No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAT4600.13305488224642922No Hit
CATTAAGACTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT4530.1310301340383314No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAAC4520.1307408842943174No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG4380.12669138787812173No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCA4350.12582363864607982No Hit
CCCTAAGAGATTGCCGTGTGGTGGAGTGAACCCCGGCCCGAATGATCCCA4340.1255343889020658No Hit
GTTTGATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGA4330.12524513915805183No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTCAACTTCAT4330.12524513915805183No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT4290.12408814018199593No Hit
GCATCAAACCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCT4270.12350964069396798No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA4260.123220390949954No Hit
GGATAGAACCTAGCAGGCATGATTAATTTTAATCTCCTTTTTTCATTAAC4250.12293114120594004No Hit
GCTTACTACTAATTCCCTGGATGCCGGGTCTTCCAAATTACTTCCCACCC4230.12235264171791209No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4200.12148489248587015No Hit
CATTGAGACTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT4160.12032789350981425No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTT4140.1197493940217863No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4090.11830314530171641No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4050.1171461463256605No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG3940.11396439914150676No Hit
GTCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG3930.11367514939749279No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC3840.111071901701367No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG3810.11020415246932506No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT3780.10933640323728314No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA3760.10875790374925517No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTT3750.10846865400524122No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTCGGAAACTTCCTATAA3720.10760090477319928No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3710.1073116550291853No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3630.1049976570770735No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG3610.10441915758904555No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA3600.10412990784503155No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACG3590.10384065810101757No Hit
CTCCTCCCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCG3570.10326215861298962No Hit
CAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGA3560.10297290886897566No Hit
GGTTTGCTCCAGACCTGCTGCGAGCAAAACAAGCCGCTAGGAGTTCCGCA3550.10268365912496166No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG3540.10239440938094771No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCATATTCATTTGAAAG3540.10239440938094771No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC3520.10181590989291975No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC3510.10152666014890577No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG3470.10036966117284987No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA3470.10036966117284987No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)