FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393528
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN67361.7116952288020169No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTCTAAATTTTAT16190.4114065581102234No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT16160.4106442235368259No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGGTGTTCTGGAT15990.40632432762090626No Hit
CATTTACATCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT14520.3689699335244252No Hit
CATCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGT13590.34533756174910046No Hit
CTTATATAATATACCCGCCTGCCAAAGAGTGTGTAAATAGTGTCTAGTTT13080.3323778740013417No Hit
CTCTTATATAATATACCCGCCTGCCAAAGAGTGTGTAAATAGTGTCTAGT12540.3186558516801854No Hit
GCCAAAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGG12090.3072208330792218No Hit
CTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA11550.2934988107580655No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT10210.2594478664796406No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACACCTAGTTAATCATTACT10070.2558903051371186No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC9970.25334918989245997No Hit
TAATATACCCGCCTGCCAAAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT9720.24699640178081356No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9250.23505316013091826No Hit
CTTTTACACAATGCGGTTATCCTGCTTTAATGCCTTTATATGCATGTATT9040.22971681811713526No Hit
GTATTAAACCTTATTATCCAGAACACCTAGTTAATCATTACTTCCAAACT9010.2289544835437377No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG8810.22387225305442052No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT8790.2233640300054888No Hit
GTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAATTCTTTGACACACT8770.2228558069565571No Hit
ATCCATAACATCCAATGACATAGCCCATAAAATTTAGAGAGTAACCCCAT8770.2228558069565571No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTT8550.21726535341830824No Hit
TTATTATCCAGAACACCTAGTTAATCATTACTTCCAAACTAGACACTATT8460.21497834969811552No Hit
CTTTCCAATCAATAGGCCGGTTAACAGGAAGTTTTCTAAAACATTCTTTG8300.2109125653066618No Hit
ATATAATATACCCGCCTGCCAAAGAGTGTGTAAATAGTGTCTAGTTTGGA7950.20201866195035675No Hit
TTCTACTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC7210.18321440913988332No Hit
GATCCATAACATCCAATGACATAGCCCATAAAATTTAGAGAGTAACCCCA7010.17813217865056616No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6870.17457461730804416No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG6830.17355817121018072No Hit
GAATACATGCATATAAAGGCATTAAAGCAGGATAACCGCATTGTGTAAAA6550.1664430485251367No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6510.16542660242727328No Hit
ATGTCATTGGATGTTATGGATCATTGCCACAAGAACACATCATACAAAAA6480.16466426785387572No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6390.16237726413368297No Hit
GTTTGTAGGCCCACTCACAGTTAATGAAAAAAGAAGATTGCAATTGATTA6380.1621231526092171No Hit
CAGTTAATGAAAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTAT6230.15831147974222928No Hit
GTGTTGTTTCTCTCTTATATAATATACCCGCCTGCCAAAGAGTGTGTAAA5870.1491634648614584No Hit
CTCTAAATTTTATGGGCTATGTCATTGGATGTTATGGATCATTGCCACAA5750.1461141265678681No Hit
CTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAG5650.14357301132320952No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT5530.14052367302961924No Hit
GTTATGGATCATTGCCACAAGAACACATCATACAAAAAATCAAAGAATGT5510.14001544998068752No Hit
AATTTAGAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATA5500.13976133845622168No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA5440.13823666930942652No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC5270.13391677339350694No Hit
CTCACAGTTAATGAAAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTT5270.13391677339350694No Hit
TTGACACACTTTCCAATCAATAGGCCGGTTAACAGGAAGTTTTCTAAAAC5250.13340855034457524No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAATCTCCTCC5170.13137565814884836No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA5160.13112154662438252No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT5130.13035921205098494No Hit
GTTATGTCAACACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTT5070.1288345429041898No Hit
TCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAA5020.1275639852818605No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA5000.12705576223292878No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGCTT5000.12705576223292878No Hit
CAATAGGCCGGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGT4980.12654753918399708No Hit
CACCAAATACTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGT4950.1257852046105995No Hit
TTGTAGGCCCACTCACAGTTAATGAAAAAAGAAGATTGCAATTGATTATG4880.1240064239393385No Hit
TTATGGATCATTGCCACAAGAACACATCATACAAAAAATCAAAGAATGTT4840.12298997784147506No Hit
GTTTTATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAAC4830.1227358663170092No Hit
CATATAAAGGCATTAAAGCAGGATAACCGCATTGTGTAAAAGGGGCAGCA4800.12197353174361164No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC4780.12146530869467992No Hit
CAATGACATAGCCCATAAAATTTAGAGAGTAACCCCATCTTTTTGTTTTG4780.12146530869467992No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAATCTTCTTTTTTCA4700.11943241649895306No Hit
ATCCAGAACACCTAGTTAATCATTACTTCCAAACTAGACACTATTTACAC4690.1191783049744872No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAATCT4690.1191783049744872No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT4640.11790774735215791No Hit
CTCCGAAAGACACCAAATACTCTATAACTGTTTCTCTTCCAAAAGTGAGA4620.1173995243032262No Hit
GTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGGTGTTCTGG4580.11638307820536276No Hit
AATATACATTTACATCAAGACATTATCAAAAAATGTGAACAGTTTGTAGG4510.11460429753410177No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4470.11358785143623833No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG4440.11282551686284076No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTCTCCATGC4400.11180907076497734No Hit
CCCCAAAAGACCCACAATTCTTTGACACACTTTCCAATCAATAGGCCGGT4330.11003029009371633No Hit
CTCTAAGGCATCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTA4330.11003029009371633No Hit
TCTAGGGACCTAGTAGTCAGTTATGTCAACACTAATATGGGCCTAAAGTT4300.10926795552031875No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT4270.10850562094692118No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG4240.10774328637352361No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTCTAA4230.10748917484905776No Hit
GCTTTATTCTACTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCT4220.1072350633245919No Hit
GGCATAATCAATTGCAATCTTCTTTTTTCATTAACTGTGAGTGGGCCTAC4210.10698095180012605No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG4090.10393161350653575No Hit
ATAATATACCCGCCTGCCAAAGAGTGTGTAAATAGTGTCTAGTTTGGAAG4060.10316927893313818No Hit
ATATTTGCCATTGGATAAGGGTATTAAACCTTATTATCCAGAACACCTAG4000.10164460978634303No Hit
GGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTCTAAA3970.10088227521294546No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA3970.10088227521294546No Hit
GGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGGCATAATCAATTG3950.10037405216401375No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)