FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393528
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19470.4947551381350247No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT19190.48764001544998065No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGGTGTTCTGGAT17750.45104795592689717No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTCTAAATTTTAT14830.3768473907828668No Hit
CTCTTATATAATATACCCGCCTGCCAAAGAGTGTGTAAATAGTGTCTAGT14020.3562643573011323No Hit
GTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAATTCTTTGACACACT13750.3494033461405541No Hit
GCCAAAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGG13610.3458457847980322No Hit
CATTTACATCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT13330.33873066211298813No Hit
CTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA13250.3366977699172613No Hit
CTTATATAATATACCCGCCTGCCAAAGAGTGTGTAAATAGTGTCTAGTTT12640.321196966924844No Hit
CATCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGT12400.3150982903376634No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG11070.28130145758370434No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT11060.2810473460592385No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC10920.2774897847167165No Hit
CTTTTACACAATGCGGTTATCCTGCTTTAATGCCTTTATATGCATGTATT10840.27545689252098965No Hit
GTTTGTAGGCCCACTCACAGTTAATGAAAAAAGAAGATTGCAATTGATTA10160.2581773088573113No Hit
GTATTAAACCTTATTATCCAGAACACCTAGTTAATCATTACTTCCAAACT9990.2538574129413917No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9860.25055396312333555No Hit
TAATATACCCGCCTGCCAAAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT9480.240897725193633No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACACCTAGTTAATCATTACT9380.2383566099489744No Hit
CTTTCCAATCAATAGGCCGGTTAACAGGAAGTTTTCTAAAACATTCTTTG9360.2378483869000427No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT8870.22539692220121565No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTT8350.21218312292899105No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC8080.20532211176841295No Hit
ATATAATATACCCGCCTGCCAAAGAGTGTGTAAATAGTGTCTAGTTTGGA7920.20125632737695917No Hit
TTATTATCCAGAACACCTAGTTAATCATTACTTCCAAACTAGACACTATT7840.19922343518123234No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7110.18067329389522474No Hit
ATCCATAACATCCAATGACATAGCCCATAAAATTTAGAGAGTAACCCCAT7030.17864040169949788No Hit
TTCTACTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC6760.17177939053891972No Hit
CTCACAGTTAATGAAAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTT6480.16466426785387572No Hit
CTCCGAAAGACACCAAATACTCTATAACTGTTTCTCTTCCAAAAGTGAGA6480.16466426785387572No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6380.1621231526092171No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6350.16136081803581956No Hit
GAATACATGCATATAAAGGCATTAAAGCAGGATAACCGCATTGTGTAAAA6350.16136081803581956No Hit
CAGTTAATGAAAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTAT6330.16085259498688784No Hit
CAATAGGCCGGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGT6290.15983614888902442No Hit
ATGTCATTGGATGTTATGGATCATTGCCACAAGAACACATCATACAAAAA6270.1593279258400927No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT6220.15805736821776342No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG6120.15551625297310484No Hit
CTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAG6080.1544998068752414No Hit
CCCCAAAAGACCCACAATTCTTTGACACACTTTCCAATCAATAGGCCGGT6020.15297513772844626No Hit
TTGTAGGCCCACTCACAGTTAATGAAAAAAGAAGATTGCAATTGATTATG5990.15221280315504868No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT5930.15068813400825354No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5900.14992579943485596No Hit
GATCCATAACATCCAATGACATAGCCCATAAAATTTAGAGAGTAACCCCA5870.1491634648614584No Hit
CTCTAAATTTTATGGGCTATGTCATTGGATGTTATGGATCATTGCCACAA5700.14484356894553882No Hit
GTGTTGTTTCTCTCTTATATAATATACCCGCCTGCCAAAGAGTGTGTAAA5650.14357301132320952No Hit
GTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGGTGTTCTGG5590.1420483421764144No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA5380.13671200016263138No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC5320.13518733101583622No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG5270.13391677339350694No Hit
GTTATGGATCATTGCCACAAGAACACATCATACAAAAAATCAAAGAATGT5160.13112154662438252No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA5150.13086743509991666No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAATCTTCTTTTTTCA5120.13010510052651908No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG5070.1288345429041898No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG4890.12426053546380435No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT4850.12324408936594093No Hit
TTGACACACTTTCCAATCAATAGGCCGGTTAACAGGAAGTTTTCTAAAAC4840.12298997784147506No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAATCTCCTCC4820.12248175479254335No Hit
GTTATGTCAACACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTT4780.12146530869467992No Hit
GGCATAATCAATTGCAATCTTCTTTTTTCATTAACTGTGAGTGGGCCTAC4720.11994063954788477No Hit
GTTTTATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAAC4640.11790774735215791No Hit
CATATAAAGGCATTAAAGCAGGATAACCGCATTGTGTAAAAGGGGCAGCA4600.11689130125429449No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4550.11562074363196519No Hit
AATTTAGAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATA4530.11511252058303348No Hit
CTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA4520.11485840905856763No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG4510.11460429753410177No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA4510.11460429753410177No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT4490.11409607448517006No Hit
GTCTTGATGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGAT4460.11333373991177248No Hit
CTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGTTTTTGCCTTCT4440.11282551686284076No Hit
ATCCAGAACACCTAGTTAATCATTACTTCCAAACTAGACACTATTTACAC4430.11257140533837491No Hit
GTTATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4300.10926795552031875No Hit
CTCTAAGGCATCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTA4290.10901384399585291No Hit
GGATAAAACCTAGCAGGCATAATCAATTGCAATCTTCTTTTTTCATTAAC4280.10875973247138704No Hit
AATATACATTTACATCAAGACATTATCAAAAAATGTGAACAGTTTGTAGG4280.10875973247138704No Hit
CCTACAAACTGTTCACATTTTTTGATAATGTCTTGATGTAAATGTATATT4270.10850562094692118No Hit
CACTCACAGTTAATGAAAAAAGAAGATTGCAATTGATTATGCCTGCTAGG4260.10825150942245533No Hit
GGATAACCGCATTGTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAAT4210.10698095180012605No Hit
ACATTGACCCTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGTTT4190.10647272875119432No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGCTT4180.10621861722672847No Hit
ATAATATACCCGCCTGCCAAAGAGTGTGTAAATAGTGTCTAGTTTGGAAG4150.1054562826533309No Hit
CACCAAATACTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGT4140.10520217112886504No Hit
ATATTTGCCATTGGATAAGGGTATTAAACCTTATTATCCAGAACACCTAG4130.10494805960439917No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA4120.10469394807993333No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTCTAA4100.1041857250310016No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAATCT4090.10393161350653575No Hit
GCTTTATTCTACTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCT4040.10266105588420647No Hit
TTATGGATCATTGCCACAAGAACACATCATACAAAAAATCAAAGAATGTT4030.1024069443597406No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4000.10164460978634303No Hit
GCTTGGGACTGTCTCGTCCCCTTCTCCGTCTGCCGTTCCGACCGACCACG3990.10139049826187717No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)