FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333979
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46941.4054775899083474No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTCTGAATTTCAT10790.32307420526440284No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGACACACT9790.2931322029229383No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8700.26049542037074186No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC8610.2578006401600101No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTT8400.2515128196683025No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC8390.25121339964488787No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGT8210.2458238392234242No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT8140.24372789905952172No Hit
GGTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA7700.2305534180292773No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA7490.22426559753756975No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA7180.21498357681171573No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC6770.2027073558517152No Hit
CTTATATAATATACCCGCCTGCCAAAGAGTGTGTAAATAGTGTCTAGTTT6700.2006114156878127No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6650.19911431557073947No Hit
GCCAAAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA6560.19641953536000764No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTG6480.1940241751726905No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6390.1913293949619587No Hit
CTCTTATATAATATACCCGCCTGCCAAAGAGTGTGTAAATAGTGTCTAGT6140.18384389437659254No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG6090.1823467942595193No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT5920.17725665386147033No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5710.17096883336976276No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC5650.1691723132292749No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5560.1664775330185431No Hit
AAGATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAA5470.16378275280781127No Hit
CTGTAACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA5360.16048913255025016No Hit
TTCTATTACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA5260.15749493231610373No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5220.15629725222244514No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5200.15569841217561584No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTAACCAATTTTCTT5160.15450073208195725No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT5090.15240479191805473No Hit
GATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC5050.15120711182439614No Hit
TAATATACCCGCCTGCCAAAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT4800.14372161123903No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC4740.14192509109854212No Hit
CCTAGTACAAAGATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTC4700.14072741100488353No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCG4660.13952973091122498No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA4600.13773321077073708No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG4520.1353378505834199No Hit
CACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGT4400.13174481030244414No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT4330.12964887013854165No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT4290.12845119004488306No Hit
CTCCGAAAGACACCAAATATTCTATTACTGTTTCTCTTCCAAAAGTGAGA4280.12815177002146844No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT4280.12815177002146844No Hit
ATATAATATACCCGCCTGCCAAAGAGTGTGTAAATAGTGTCTAGTTTGGA4240.12695408992780985No Hit
CACTCATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTT4240.12695408992780985No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT4180.12515756978732195No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG4180.12515756978732195No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT4170.12485814976390731No Hit
GTTATGGGTCATTGCCACAAGAACACATCACACAAAAAATCAAAGAATGT4160.12455872974049267No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGAACACATCACACAAAAA4060.12156452950634622No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGTGTGATGTGTT4000.11976800936585832No Hit
GCCATAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGCC3920.11737264917854116No Hit
GTATATGGTGACCCACAAAATGAGGCGCTATGTGTTGTTTCTCTCTTATA3890.11647438910829724No Hit
CTCTTGGACTCTCTGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA3870.11587554906146794No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT3860.1155761290380533No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC3840.11497728899122399No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT3830.11467786896780935No Hit
CATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAA3800.11377960889756543No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG3750.11228250878049217No Hit
GTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG3750.11228250878049217No Hit
AGTATATGGTGACCCACAAAATGAGGCGCTATGTGTTGTTTCTCTCTTAT3730.1116836687336629No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3710.1110848286868336No Hit
GACATAGCCCATGAAATTCAGAGAGTAACCCCATCTTTTTGTTTTGTTAG3700.11078540866341896No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT3690.1104859886400043No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCACCTCGCAGACGAAGAT3680.11018656861658967No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3660.10958772856976037No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC3660.10958772856976037No Hit
GGGTTACTCTCTGAATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT3610.10809062845268715No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC3610.10809062845268715No Hit
GAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGT3530.10569526826536997No Hit
ACCCATAACATCCAATGACATAGCCCATGAAATTCAGAGAGTAACCCCAT3530.10569526826536997No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCT3520.10539584824195533No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGAACACATCACACA3520.10539584824195533No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAATCTTCTTTTCTCA3480.10419816814829674No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT3480.10419816814829674No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG3470.1038987481248821No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC3470.1038987481248821No Hit
CTATTACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAG3470.1038987481248821No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT3460.10359932810146746No Hit
ATTTTGTGGTCTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACA3460.10359932810146746No Hit
ACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTT3450.10329990807805281No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTCTATTA3440.10300048805463816No Hit
CCTACTATACATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGT3400.10180280796097958No Hit
CTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCAAGCAATTCTT3360.100605127867321No Hit
CAGTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTAT3360.100605127867321No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT3350.10030570784390636No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)