FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333979
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGACACACT12340.36948430889367295No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTCTGAATTTCAT10100.3024142236487923No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8960.26828034097952264No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT8050.2410331188487899No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTT7930.23744007856781416No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7260.21737893699903288No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGT7250.21707951697561823No Hit
CTTATATAATATACCCGCCTGCCAAAGAGTGTGTAAATAGTGTCTAGTTT6950.20809691627317883No Hit
GCCAAAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA6930.20749807622634955No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTG6860.20540213606244703No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA6850.20510271603903238No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC6690.20031199566439806No Hit
CTCTTATATAATATACCCGCCTGCCAAAGAGTGTGTAAATAGTGTCTAGT6620.19821605550049554No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG6410.19192823500878797No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC6320.18923345479805617No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6200.1856404145170804No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6170.18474215444683648No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT6170.18474215444683648No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA5860.17546013372098246No Hit
GGTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA5730.17156767341659204No Hit
TAATATACCCGCCTGCCAAAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT5580.16707637306537237No Hit
CTGTAACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA5540.1658786929717138No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5520.1652798529248845No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC5490.16438159285464057No Hit
TTCTATTACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA5460.1634833327843966No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT5420.16228565269073802No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5410.1619862326673234No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5200.15569841217561584No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC5120.15330305198829866No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA5110.153003631964884No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT5070.15180595187122545No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTAACCAATTTTCTT4930.1476140715434204No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG4890.14641639144976182No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4880.14611697142634716No Hit
CTCTTGGACTCTCTGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA4790.14342219121561536No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4700.14072741100488353No Hit
CTCTCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACCACGGGGCGCACCT4640.13893089086439567No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG4630.138631470840981No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCG4620.1383320508175664No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4610.13803263079415173No Hit
GATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC4600.13773321077073708No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT4590.13743379074732243No Hit
GTATATGGTGACCCACAAAATGAGGCGCTATGTGTTGTTTCTCTCTTATA4560.1365355306770785No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT4480.13414017048976132No Hit
ATATAATATACCCGCCTGCCAAAGAGTGTGTAAATAGTGTCTAGTTTGGA4400.13174481030244414No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG4400.13174481030244414No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4380.13114597025561486No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT4340.12994829016195628No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC4300.12875061006829772No Hit
CACTCATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTT4220.12635524988098054No Hit
GTTATGGGTCATTGCCACAAGAACACATCACACAAAAAATCAAAGAATGT4200.12575640983415126No Hit
CTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCAAGCAATTCTT4190.1254569898107366No Hit
CCTAGTACAAAGATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTC4170.12485814976390731No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT4160.12455872974049267No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4160.12455872974049267No Hit
CTCCGAAAGACACCAAATATTCTATTACTGTTTCTCTTCCAAAAGTGAGA4150.12425930971707802No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG4140.12395988969366337No Hit
AAGATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAA4090.12246278957659014No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4080.1221633695531755No Hit
ATTTTGTGGTCTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACA4070.12186394952976086No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTCTATTA4060.12156452950634622No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT4040.12096568945951691No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGAACACATCACACAAAAA4020.12036684941268763No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4010.12006742938927296No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT3980.11916916931902904No Hit
GTTTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTA3890.11647438910829724No Hit
CCTACTATACATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGT3870.11587554906146794No Hit
CACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGT3870.11587554906146794No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC3840.11497728899122399No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC3830.11467786896780935No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGAACACATCACACA3780.11318076885073612No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG3770.11288134882732148No Hit
CATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAA3730.1116836687336629No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC3680.11018656861658967No Hit
ACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTT3660.10958772856976037No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT3620.10839004847610179No Hit
CTATTACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAG3610.10809062845268715No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3590.10749178840585787No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT3590.10749178840585787No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACCGTGG3580.1071923683824432No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGTGTGATGTGTT3550.10629410831219928No Hit
GATTGAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGT3520.10539584824195533No Hit
CTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACCACGGGGCGCAC3520.10539584824195533No Hit
CTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC3510.10509642821854069No Hit
AGTATATGGTGACCCACAAAATGAGGCGCTATGTGTTGTTTCTCTCTTAT3460.10359932810146746No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3450.10329990807805281No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC3440.10300048805463816No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA3430.10270106803122352No Hit
GGGTTACTCTCTGAATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT3430.10270106803122352No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG3430.10270106803122352No Hit
GCCATAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGCC3400.10180280796097958No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC3390.10150338793756494No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCT3380.1012039679141503No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT3380.1012039679141503No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT3350.10030570784390636No Hit
GGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGTCTTTTGGGTTTT3340.10000628782049172No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)