FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376397
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC25080.6663177442965805No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18980.5042548160585765No Hit
CCTCCAGCTTATAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGA15650.4157843978565185No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA13520.359195211438986No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC12980.34484865713594953No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCATGC12160.3230631487498572No Hit
CATTTAAACCCTACCAAAACTAAAAGATGGGGTTACTCTTTACATTTCAT10910.28985353230764327No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA10760.28586837833457757No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT10630.2824145782245873No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC9600.2550498542762031No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9520.25292443882390137No Hit
CTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGA8920.23698382293163867No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACCACCGTG8630.22927919191704504No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG8500.2258253918070548No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAG8330.2213088839709137No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA7670.20377420648942474No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC7500.1992576986532836No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA7320.19447551388560483No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGGCAAGAAATGTGAAACCACA7290.19367848309099167No Hit
GCGTGGGACCATGCAGAACCTGCACGACTACTGTTCAAGGAACCTCTATG7150.18995900604946372No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7150.18995900604946372No Hit
ATATAGAGCTGAGGCGGTATCAAGAAGATCTCGTACTGAAGGAAAGAAGT7140.18969332911792602No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT6920.18384843662409636No Hit
CTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAA6920.18384843662409636No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT6730.17880057492487986No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT6670.1772065133356536No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6640.17640948254104044No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6570.17454974402027645No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6370.16923620538952222No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCATCA6150.1633913128956926No Hit
GCTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG6020.1599375127857023No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAACCTTCCACCAAACTCTACAAG6000.1594061589226269No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6000.1594061589226269No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5950.15807777426493835No Hit
CTAGTAAACTGAGCCAAGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5930.15754642040186292No Hit
CATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACT5920.15728074347032522No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT5840.1551553280180235No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5830.1548896510864858No Hit
GTCCCACGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC5820.15462397415494808No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5710.15170152790803326No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCATCATACTGCACTCAGGCA5570.14798205086650532No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC5570.14798205086650532No Hit
ACATAGAGGTTCCTTGAACAGTAGTCGTGCAGGTTCTGCATGGTCCCACG5560.1477163739349676No Hit
CTCTAAGGCTTCCCGATATAGAGCTGAGGCGGTATCAAGAAGATCTCGTA5550.1474506970034299No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG5500.14612231234574133No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5490.1458566354142036No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTGTCCGTCCCGAGAATGTTT5440.14452825075651507No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT5330.14160580450960025No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5320.14134012757806252No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATA5290.14054309678344937No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5260.13974606598883627No Hit
TCTTGATACCGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGC5160.13708929667345912No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT5130.13629226587884602No Hit
GCTTTAATGCCCTTGTATGCCTGTATTCAATCTAAGCAGGCTTTCACTTT5130.13629226587884602No Hit
GTGTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTG5060.13443252735808203No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4970.13204143497424262No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4930.1309787272480918No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGACGAAACGG4910.13044737338501636No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT4900.13018169645347863No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT4870.12938466565886553No Hit
ACTACACACCGTGCCAACAAATCCGCCTCCTGCATCTACCAATCGCCAGT4750.12619654248041295No Hit
GTTTAATACCCTTATCTAAAGGCAAATATTTGGTAACATTTGGATAAAAC4730.12566518861733755No Hit
TTAAAGCCCTTGTATGCCTGTATTCAATCTAAGCAGGCTTTCACTTTCTC4720.12539951168579982No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT4720.12539951168579982No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA4670.12407112702811128No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4630.12300841930196042No Hit
CTACACACCGTGCCAACAAATCCGCCTCCTGCATCTACCAATCGCCAGTC4630.12300841930196042No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4480.11902326532889475No Hit
TCCTTGAACAGTAGTCGTGCAGGTTCTGCATGGTCCCACGCTGGTGGTTG4480.11902326532889475No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAATCTCCTCC4470.11875758839735705No Hit
CCTAATGCCCCTATCTTATCAACACTTCCGGAGACTACTGTTGTTAGACG4460.11849191146581933No Hit
TCATCATACTGCACTCAGGCAAGCAATCCTTTGCTGGGGGGAGCTAATGA4420.11742920373966849No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC4400.11689784987659307No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4350.1155694652189045No Hit
GATACATAGAGGTTCCTTGAACAGTAGTCGTGCAGGTTCTGCATGGTCCC4330.11503811135582909No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4320.11477243442429139No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC4280.11370972669814051No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT4230.11238134204045197No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT4210.11184998817737656No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG4130.10972457272507485No Hit
GTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGT4130.10972457272507485No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT4110.10919321886199942No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG4070.10813051113584858No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4050.10759915727277317No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4040.10733348034123544No Hit
ATACTGCACTCAGGCAAGCAATCCTTTGCTGGGGGGAGCTAATGACTCTA4030.10706780340969775No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA4030.10706780340969775No Hit
ATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCATCATACTG4010.10653644954662232No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3970.10547374182047146No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3950.10494238795739604No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG3950.10494238795739604No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT3940.10467671102585833No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA3930.10441103409432062No Hit
GTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA3890.10334832636816978No Hit
GTTCAAGGAACCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGA3860.10255129557355663No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3840.10201994171048122No Hit
GTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGT3830.10175426477894352No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT3800.10095723398433037No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)