FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376397
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC21100.5605783255445713No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18950.5034577852639633No Hit
CCTCCAGCTTATAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGA14920.39638998185426555No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA13890.36902525790588125No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC11930.31695257932448984No Hit
CATTTAAACCCTACCAAAACTAAAAGATGGGGTTACTCTTTACATTTCAT10790.28666540912919075No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT10570.28082051663536106No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA9530.25319011575543904No Hit
CTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGA9090.24150033076777974No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCATGC9010.23937491531547805No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG8620.22901351498550732No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACCACCGTG8460.22476268408090394No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA7850.20855639125710357No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7840.20829071432556584No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7810.20749368353095268No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7560.2008517602425099No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA7420.19713228320098192No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGGCAAGAAATGTGAAACCACA7190.19102171377561458No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC7050.1873022367340866No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC7020.18650520593947348No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG7010.18623952900793578No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC6910.18358275969255866No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT6870.1825200519664078No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT6790.18039463651410612No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT6760.179597605719493No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAG6590.17508109788335188No Hit
GCGTGGGACCATGCAGAACCTGCACGACTACTGTTCAAGGAACCTCTATG6580.17481542095181418No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT6450.17136162084182394No Hit
ATATAGAGCTGAGGCGGTATCAAGAAGATCTCGTACTGAAGGAAAGAAGT6270.16657943607414513No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCATCA6270.16657943607414513No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6220.16525105141645655No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAACCTTCCACCAAACTCTACAAG6190.16445402062184344No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC6160.1636569898272303No Hit
GCTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG6120.16259428210107946No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCATCATACTGCACTCAGGCA5880.15621803574417437No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5780.15356126642879725No Hit
CTAGTAAACTGAGCCAAGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5720.151967204839571No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT5650.150107466318807No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5500.14612231234574133No Hit
GTCCCACGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC5440.14452825075651507No Hit
CATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACT5400.1434655430303642No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT5380.14293418916728878No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5360.14240283530421335No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5250.13948038905729854No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT5240.13921471212576084No Hit
GCTTTAATGCCCTTGTATGCCTGTATTCAATCTAAGCAGGCTTTCACTTT5170.13735497360499685No Hit
CTCTAAGGCTTCCCGATATAGAGCTGAGGCGGTATCAAGAAGATCTCGTA5170.13735497360499685No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5150.13682361974192142No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATA5140.13655794281038372No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5060.13443252735808203No Hit
TCTTGATACCGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGC5030.13363549656346888No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT5020.13336981963193117No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT4960.1317757580427049No Hit
TCCCGGGGCCGCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCG4930.1309787272480918No Hit
ACATAGAGGTTCCTTGAACAGTAGTCGTGCAGGTTCTGCATGGTCCCACG4750.12619654248041295No Hit
GTGTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTG4730.12566518861733755No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4720.12539951168579982No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG4710.12513383475426212No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT4640.12327409623349814No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4610.122477065438885No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTGTCCGTCCCGAGAATGTTT4590.12194571157580958No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4570.12141435771273415No Hit
TCATCATACTGCACTCAGGCAAGCAATCCTTTGCTGGGGGGAGCTAATGA4560.12114868078119646No Hit
TTAAAGCCCTTGTATGCCTGTATTCAATCTAAGCAGGCTTTCACTTTCTC4490.11928894226043248No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA4440.1179605576027439No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4410.11716352680813076No Hit
GTCTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATAC4400.11689784987659307No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4390.11663217294505535No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4370.11610081908197993No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA4340.1153037882873668No Hit
TCCTTGAACAGTAGTCGTGCAGGTTCTGCATGGTCCCACGCTGGTGGTTG4320.11477243442429139No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCTTCCCGGGGCCG4300.11424108056121594No Hit
GTTTAATACCCTTATCTAAAGGCAAATATTTGGTAACATTTGGATAAAAC4290.11397540362967824No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4280.11370972669814051No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4230.11238134204045197No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA4210.11184998817737656No Hit
CTACACACCGTGCCAACAAATCCGCCTCCTGCATCTACCAATCGCCAGTC4190.11131863431430111No Hit
ACTACACACCGTGCCAACAAATCCGCCTCCTGCATCTACCAATCGCCAGT4150.11025592658815027No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC4140.10999024965661257No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT4100.10892754193046172No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT4080.10839618806738628No Hit
CCTAATGCCCCTATCTTATCAACACTTCCGGAGACTACTGTTGTTAGACG4060.10786483420431087No Hit
GTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGT4060.10786483420431087No Hit
ATACTGCACTCAGGCAAGCAATCCTTTGCTGGGGGGAGCTAATGACTCTA4050.10759915727277317No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT3950.10494238795739604No Hit
GTTCAAGGAACCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGA3950.10494238795739604No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC3930.10441103409432062No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC3910.10387968023124519No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT3900.10361400329970749No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT3880.10308264943663208No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG3830.10175426477894352No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT3790.10069155705279267No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC3790.10069155705279267No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGACGAAACGG3780.10042588012125495No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)