FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392109
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15230.38841240573411984No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13310.33944642943671277No Hit
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT11520.2937958577844426No Hit
ATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATACCCCATGAA10500.2677826828764451No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA10190.2598767179534262No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG9890.25222578415695635No Hit
GCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAAAG9640.24585000599323148No Hit
GTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT9300.23717894769056563No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8710.2221321112241749No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8250.21040067940292112No Hit
CCTATTAGCAATGTCTTCATGTAAATGAATTTTAGGAAAAGAAGGGGTTT7450.1899981892790015No Hit
GGATCATATTGTACAAAAAATCAAACAATGTTTTAGAAAACTTCCTGTCA7190.18336737998872762No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACCCGACATTATT7120.18158216210288466No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG6970.17775669520464973No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG6850.17469632168606178No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAGTACCACATC6740.17189097929402283No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTT6590.1680655123957879No Hit
CCATTTACACAATGTGGTTACCCTGCATTAATGCCTTTGTATGCATGTAT6490.16551520113029797No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6440.16424004549755297No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTGAATTTCAT5950.1517435202966522No Hit
GTACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA5630.14358252424708434No Hit
CTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGC5510.14052215072849641No Hit
TTCTTCTACTGTCCCTATCTTTAATCCTGATTGGCAAACCCCTTCTTTTC5510.14052215072849641No Hit
ATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATATT5500.1402671196019474No Hit
CTTTAATCCTGATTGGCAAACCCCTTCTTTTCCTAAAATTCATTTACATG5390.13746177720990846No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5310.1354215281975165No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC5290.13491146594441852No Hit
ACATCATGGGAGGTTGGTCATCAAAACCTCGCAAAGGCATGGGGACGAAC5270.13440140369132053No Hit
CTACTAGACACAGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGA5270.13440140369132053No Hit
GTATACATGCATACAAAGGCATTAATGCAGGGTAACCACATTGTGTAAAT5250.13389134143822254No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAGCTTTCCACCAAGCTCTGCAAG4960.1264954387683017No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCA4870.12420015862936072No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT4860.12394512750281171No Hit
GTAGAAGAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGAAT4800.12241494074351775No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATACTCAAGGA4680.11935456722492979No Hit
CATGAAGACATTGCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGT4660.11884450497183183No Hit
GTTTGATGTAGCTCTTGTTCCCAAGAATATGGTGACCCGCAAAATGAGGC4590.11705928708598884No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4580.11680425595943986No Hit
ATACAGCCCCAAGCGGCCTCGCGAGGGGTCGTCCGCGGGATTCAGCGCTG4570.11654922483289086No Hit
ATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAGTG4550.11603916257979287No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACA4500.1147640069470479No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT4460.1137438824408519No Hit
GTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAA4430.11297878906120491No Hit
GTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCAGG4400.11221369568155794No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG4370.11144860230191095No Hit
GTCTTCATGTAAATGAATTTTAGGAAAAGAAGGGGTTTGCCAATCAGGAT4340.11068350892226397No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACAGCACTCAGGCA4300.10966338441606797No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC4290.10940835328951898No Hit
GTTTTGATGACCAACCTCCCATGATGTAGCTCTTGTTCCCAAGAATATGG4190.10685804202402903No Hit
GGCCTAAAGATCAGGCAACTATTGTGGTTTCATATTTCTTGCCTTACTTT4150.10583791751783306No Hit
CTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGCTT4100.10456276188508809No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACACTCAACCCTGTTCCAA4040.1030325751257941No Hit
GGGCTGTATCGTCCCCTTCTCCGTCTGCCGTACCGTCCGACCACGGGGCG4040.1030325751257941No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4020.10252251287269612No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4010.10226748174614712No Hit
GGATTAAAGATAGGGACAGTAGAAGAATAAAGCCCAGTAAAATTTCCCAC3950.10073729498685313No Hit
CTCTTTGGAAGGCTGGGATTCTATATAAGAGGGAAACTACACGTAGCGCC3950.10073729498685313No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)