FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268217
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12470.46492205937729525No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA12460.4645492269319245No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG8910.33219370882531685No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA8570.31951740568271214No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG7760.28931797760768335No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7750.2889451451623126No Hit
ATGTAAGACCTTGGGCAGGACCTGATGGGCGTTCACGGTGGTTTCCATGC7630.2844711558178639No Hit
ATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATAACCCATGAA6920.2580000521965423No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAGTACCACATC6540.24383241927245478No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCA6480.24159542460023042No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG6300.23488444058355737No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACAGCACTCAGGCA6220.23190178102059153No Hit
ACTGTGGAGTTGCTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG6190.23078328368447937No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6190.23078328368447937No Hit
GTACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA6060.2259364618946599No Hit
GGATCATATTGTACAAAAAATCAAACACTGTTTTAGAAAACTTCCTGTCA6040.22519079700391847No Hit
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT5960.22220813744095266No Hit
GGGCTGTATCGTCCCCTTCTCCGTCTGCCGTTCCGTCCGACCACGGGGCG5880.21922547787798685No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTT5740.2140058236427967No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG5730.213632991197426No Hit
GTGTAAATGGAGCTGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT5680.21176882897057234No Hit
CCTATTAGCAATGTCTTCATGTAAATGAATTTTAGGAAAAGAAGGGGTTT5570.20766767207149434No Hit
ATACAGCCCCAAGCGGCCTCGCGAGGGGTCGTCCGCGGGATTCAGCGCCG5560.20729483962612363No Hit
GTAGAAGAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGAAT5470.2039393476177871No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG5440.20282085028167493No Hit
ATATAGAGCTGAGGCTGTATCTAGTAGATCCCGGACGGAAGGAAAGAAGT5390.20095668805482128No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5360.19983819071870912No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTAAATTTCAT5230.19499136892888969No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5200.1938728715927775No Hit
CTCTAAGGCTTCCCGATATAGAGCTGAGGCTGTATCTAGTAGATCCCGGA5160.19238154181129458No Hit
CCATTTACACAATGTGGTTACCCTGCATTAATGCCTTTGTATGCATGTAT4970.1852977253492508No Hit
GGCCTAAAGATCAGGCAATTATTGTGGTTTCATATTTCTTGCCTTACTTT4770.17784107644183628No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATACTCAAGGA4730.17634974666035338No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAGCTTTTCACCAAGCTCTGCAAG4590.1711300924251632No Hit
TCCTAATACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGT4550.1696387626436803No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAGGACCTGATGGGCGTTC4550.1696387626436803No Hit
CTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGC4530.16889309775293884No Hit
ACTAGATACAGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGC4520.1685202653075681No Hit
GCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCAATGTAAATGAAAAAAG4490.16740176797145595No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACCCGACATTATT4410.16441910840849014No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC4390.16367344351774868No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4350.1621821137362658No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT4330.16143644884552433No Hit
GTGCCAGCAGTGCCTCCTCCTGCCTCCACCAATCGGCAGTCAGGAAGGCA4220.15733529194644635No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4200.1565896270557049No Hit
CATGTATACAAGCGAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4190.15621679461033416No Hit
ATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATATT4090.1524884701566269No Hit
GTATACATGCATACAAAGGCATTAATGCAGGGTAACCACATTGTGTAAAT4020.1498786430390318No Hit
CTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGCTT3980.14838731325754895No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC3960.14764164836680746No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCT3960.14764164836680746No Hit
CTGCTGGCACTGTGGCCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAA3950.14726881592143673No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG3920.14615031858532457No Hit
CTACTAGATACAGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGA3900.1454046536945831No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG3890.14503182124921238No Hit
ACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGGAAATTGC3860.14391332391310022No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT3850.1435404914677295No Hit
GGATTAAAGATAGGGACAGTAGAAGAATAAAGCCCAGTAAAATTTCCCAC3760.14018499945939294No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCGAAACAGGCTTTTACTTT3750.13981216701402222No Hit
ATACAGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTAATGACTCTA3730.13906650212328078No Hit
ATACTAACATGGGCCTAAAGATCAGGCAATTATTGTGGTTTCATATTTCT3720.13869366967791005No Hit
GCACGGGACCCTGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG3690.13757517234179786No Hit
GTCGTTGACATTGCTGGGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG3650.13608384256031497No Hit
CAGTTGGACCCTGCATTCGGAGCCAATTCAAACAATCCAGATTGGGACTT3650.13608384256031497No Hit
GTCCCGTGCTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC3560.13272835055197843No Hit
GGGTTGAGTGTTCCTGAACTGGAGCCACCAGCAGGAAAATACAGGCCCCT3530.13160985321586627No Hit
GTGCTGTATGGTGAGGTGAGCAATGCTCAGGAGACTCTAAGGCTTCCCGA3510.1308641883251248No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGACGGAACGG3470.1293728585436419No Hit
GGTTGGGGCTTGGCCATCGGCCATCAGCGCATGCGTGGAACCTTTGTGGC3470.1293728585436419No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG3470.1293728585436419No Hit
GTCTTTAAACACACAGTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACA3450.12862719365290046No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGGCAAGAAATATGAAACCACA3420.12750869631678827No Hit
CCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAG3360.12527170164456392No Hit
GAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGAATACTAAC3330.12415320430845174No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG3330.12415320430845174No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG3330.12415320430845174No Hit
GGGTAAAGGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA3330.12415320430845174No Hit
GGCATGGGGACGAACCTTTCTGTTCCCAACCCTCTGGGATTCTTTCCCGA3280.12228904208159812No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3280.12228904208159812No Hit
CCTCTGCACGTTGCATGGAAACCACCGTGAACGCCCATCAGGTCCTGCCC3250.12117054474548594No Hit
GTTTTGGGGTGGAGCCCTCAGGCTCAGGGCATATTGGCCACAGTGCCAGC3240.12079771230011521No Hit
CTTTAGGCCCATGTTAGTATTAACATAATTGACTACTAGATCCCTGGATG3220.12005204740937377No Hit
GAGCTACATCATGGGAGGTTGGTCAACAAAACCTCGCAAAGGCATGGGGA3210.11967921496400304No Hit
CATGAAGACATTGCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCAATGT3190.11893355007326156No Hit
ACCTTGGGCAGGACCTGATGGGCGTTCACGGTGGTTTCCATGCAACGTGC3180.11856071762789086No Hit
TTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACCCCTTCTTTTC3170.11818788518252012No Hit
GACCAGGCCGTTGCCGGGCAACGGGGTAAAGGTTCATATACTGTTTACTT3170.11818788518252012No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC3160.11781505273714939No Hit
TCACCATACAGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTAATGA3160.11781505273714939No Hit
TGTTAATACTAACATGGGCCTAAAGATCAGGCAATTATTGTGGTTTCATA3150.11744222029177868No Hit
GTTTTAGAAAACTTCCTGTCAGTCGACCTATTGATTGGAAAGTATGTCAG3130.11669655540103721No Hit
ACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGAGTTGCTCTCATTT3130.11669655540103721No Hit
CCCCAAAACACCTCCGTGTGGGGGAGTGAACCCTGGCCCGAATGCTCCCA3110.11595089051029576No Hit
GGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA3090.1152052256195543No Hit
GAATTATCAGTCCCGATGAGTTTTGCTCCAGACCGGCTGCGAGCAAAACA3080.11483239317418359No Hit
AGTTGGAACAGGGTTGAGTGTTCCTGAACTGGAGCCACCAGCAGGAAAAT3070.11445956072881286No Hit
ATGTTAATACTAACATGGGCCTAAAGATCAGGCAATTATTGTGGTTTCAT3060.11408672828344213No Hit
GTACAGACTTGGCCCCCAGTACCACATCATCCATATAGCTGAAAGCCAAA3020.11259539850195924No Hit
GCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCAGGCC3020.11259539850195924No Hit
GTATATTTCCGAGAAAGGACGACAGAATTATCAGTCCCGATGAGTTTTGC3010.11222256605658851No Hit
ATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAGTG2980.11110406872047633No Hit
GGCCTGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGC2950.10998557138436416No Hit
GGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAGACGGGGA2950.10998557138436416No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA2930.1092399064936227No Hit
ACCTAGCAGCCATGGAAACGATGTATATTTCCGAGAAAGGACGACAGAAT2920.10886707404825198No Hit
GATCCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCCGGTCTG2910.10849424160288125No Hit
GGAAAATACAGGCCCCTGACTCTGGGATCTTGCAGAGCTTGGTGAAAAGC2910.10849424160288125No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACACTCAACCCTGTTCCAA2870.10700291182139834No Hit
CTCTTATGTAAGACCTTGGGCAGGACCTGATGGGCGTTCACGGTGGTTTC2850.1062572469306569No Hit
CTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAAGA2790.10402025225843255No Hit
GAATACAGGTGCAATTTCCATCCGTAGGTTTTGTACAGCAACATGAGGGA2760.10290175492232034No Hit
TACTAACATGGGCCTAAAGATCAGGCAATTATTGTGGTTTCATATTTCTT2750.10252892247694964No Hit
CGTAAAGAGAGGTGCGCCCCGTGGTCGGACGGAACGGCAGACGGAGAAGG2740.10215609003157891No Hit
ACATAGAGTTGCCTTGAGCAGGAGTCGTGCAGGTTTTGCAGGGTCCCGTG2710.10103759269546674No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT2700.10066476025009599No Hit
TGATGATGGTGCGCAAACCAATTTATGCCTACAGCCTCCTAATACAAAGA2690.10029192780472529No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)