FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268217
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12120.4518729237893198No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11200.41757233881521305No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG10390.38737291074018426No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA9870.3679856235809065No Hit
GGGCTGTATCGTCCCCTTCTCCGTCTGCCGTTCCGTCCGACCACGGGGCG9440.3519538284299653No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG8880.33107521148920466No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA8380.31243358922066833No Hit
ATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATAACCCATGAA7570.28223416114563954No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACAGCACTCAGGCA6720.25054340328912783No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCA6560.24457808416319624No Hit
GTGTAAATGGAGCTGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT6540.24383241927245478No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6470.2412225921548597No Hit
ACTGTGGAGTTGCTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG6430.23973126237337677No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG6220.23190178102059153No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAGTACCACATC6160.22966478634836718No Hit
ATACAGCCCCAAGCGGCCTCGCGAGGGGTCGTCCGCGGGATTCAGCGCCG6130.22854628901225502No Hit
GGATCATATTGTACAAAAAATCAAACACTGTTTTAGAAAACTTCCTGTCA6120.22817345656688426No Hit
GTACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA6010.2240722996678063No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG5730.213632991197426No Hit
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT5410.20170235294556274No Hit
ATGTAAGACCTTGGGCAGGACCTGATGGGCGTTCACGGTGGTTTCCATGC5380.20058385560945058No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5350.1994653582733384No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTT5260.19610986626500182No Hit
CCTATTAGCAATGTCTTCATGTAAATGAATTTTAGGAAAAGAAGGGGTTT5240.19536420137426042No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5170.19275437425666533No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAGCTTTTCACCAAGCTCTGCAAG5130.19126304447518241No Hit
CTCTAAGGCTTCCCGATATAGAGCTGAGGCTGTATCTAGTAGATCCCGGA5050.1882803849122166No Hit
CCATTTACACAATGTGGTTACCCTGCATTAATGCCTTTGTATGCATGTAT5040.18790755246684587No Hit
ATATAGAGCTGAGGCTGTATCTAGTAGATCCCGGACGGAAGGAAAGAAGT5010.18678905513073368No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATACTCAAGGA4990.18604339023999225No Hit
GGCCTAAAGATCAGGCAATTATTGTGGTTTCATATTTCTTGCCTTACTTT4820.1797052386686899No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACCCGACATTATT4810.17933240622331917No Hit
CTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGC4750.17709541155109482No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCT4720.17597691421498265No Hit
GTAGAAGAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGAAT4570.17038442753442176No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC4470.1666561030807145No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG4460.16628327063534376No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTAAATTTCAT4440.16553760574460233No Hit
GTGCCAGCAGTGCCTCCTCCTGCCTCCACCAATCGGCAGTCAGGAAGGCA4330.16143644884552433No Hit
ATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATATT4210.15696245950107562No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4190.15621679461033416No Hit
GCACGGGACCCTGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG4170.15547112971959273No Hit
GTATACATGCATACAAAGGCATTAATGCAGGGTAACCACATTGTGTAAAT4160.155098297274222No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG4160.155098297274222No Hit
ACTAGATACAGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGC4160.155098297274222No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC4130.1539797999381098No Hit
GCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCAATGTAAATGAAAAAAG4070.15174280526588546No Hit
GTATCGTCCCCTTCTCCGTCTGCCGTTCCGTCCGACCACGGGGCGCACCT4030.15025147548440254No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT3980.14838731325754895No Hit
CATGTATACAAGCGAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC3960.14764164836680746No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCGAAACAGGCTTTTACTTT3860.14391332391310022No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3840.14316765902235876No Hit
GGTTGGGGCTTGGCCATCGGCCATCAGCGCATGCGTGGAACCTTTGTGGC3830.14279482657698803No Hit
CTGCTGGCACTGTGGCCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAA3830.14279482657698803No Hit
CTACTAGATACAGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGA3750.13981216701402222No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG3720.13869366967791005No Hit
GTGCTGTATGGTGAGGTGAGCAATGCTCAGGAGACTCTAAGGCTTCCCGA3660.1364566750056857No Hit
CCTCTGCACGTTGCATGGAAACCACCGTGAACGCCCATCAGGTCCTGCCC3650.13608384256031497No Hit
GGGTAAAGGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA3600.13421968033346135No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT3580.1334740154427199No Hit
ATACAGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTAATGACTCTA3580.1334740154427199No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTGCACTTCGCTTCAC3550.13235551810660773No Hit
ATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAGTG3550.13235551810660773No Hit
CTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGCTT3550.13235551810660773No Hit
GGCCTGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGC3520.1312370207704955No Hit
ACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGGAAATTGC3490.13011852343438335No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG3490.13011852343438335No Hit
TTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACCCCTTCTTTTC3460.12900002609827116No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG3460.12900002609827116No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC3450.12862719365290046No Hit
CTTTAGGCCCATGTTAGTATTAACATAATTGACTACTAGATCCCTGGATG3440.12825436120752973No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGGCAAGAAATATGAAACCACA3370.12564453408993465No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG3340.12452603675382247No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAGGACCTGATGGGCGTTC3320.12378037186308102No Hit
GAAATATACATCGTTTCCATGGCTGCTAGGTTGTACTGCCAACTGGATTC3320.12378037186308102No Hit
GCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCAGGCC3320.12378037186308102No Hit
GATCCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCCGGTCTG3290.12266187452696882No Hit
GGATTAAAGATAGGGACAGTAGAAGAATAAAGCCCAGTAAAATTTCCCAC3280.12228904208159812No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA3250.12117054474548594No Hit
GGAAAATACAGGCCCCTGACTCTGGGATCTTGCAGAGCTTGGTGAAAAGC3250.12117054474548594No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACACTCAACCCTGTTCCAA3230.12042487985474447No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3220.12005204740937377No Hit
ATACTAACATGGGCCTAAAGATCAGGCAATTATTGTGGTTTCATATTTCT3210.11967921496400304No Hit
GCTTGGGGCTGTATCGTCCCCTTCTCCGTCTGCCGTTCCGTCCGACCACG3200.11930638251863229No Hit
CATGAAGACATTGCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCAATGT3180.11856071762789086No Hit
CCCCAAAACACCTCCGTGTGGGGGAGTGAACCCTGGCCCGAATGCTCCCA3160.11781505273714939No Hit
GTTTTGGGGTGGAGCCCTCAGGCTCAGGGCATATTGGCCACAGTGCCAGC3140.11706938784640794No Hit
GGGTTGAGTGTTCCTGAACTGGAGCCACCAGCAGGAAAATACAGGCCCCT3130.11669655540103721No Hit
GGGTAACCACATTGTGTAAATGGAGCTGCAAAGCCCAAAAGACCCACAAT3130.11669655540103721No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCGCGAGGCCG3120.11632372295566651No Hit
GTCCCGTGCTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC3100.11557805806492503No Hit
CAGTTGGACCCTGCATTCGGAGCCAATTCAAACAATCCAGATTGGGACTT3090.1152052256195543No Hit
GTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGCCATCG3080.11483239317418359No Hit
GTCTTTAAACACACAGTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACA3040.11334106339270068No Hit
CTGCTAGGTTGTACTGCCAACTGGATTCTTCGCGGGACGTCCTTTGTTTA3000.11184973361121778No Hit
TGTTAATACTAACATGGGCCTAAAGATCAGGCAATTATTGTGGTTTCATA2990.11147690116584705No Hit
GTTCCAACTATTGCCTCTCACATCTCGTCAATCTCCTCGAGGATTGGGGA2990.11147690116584705No Hit
TCCTAATACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGT2990.11147690116584705No Hit
GCCTAATCATCTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGC2950.10998557138436416No Hit
GACCAGGCCGTTGCCGGGCAACGGGGTAAAGGTTCATATACTGTTTACTT2940.10961273893899343No Hit
CTCCTGAGCATTGCTCACCTCACCATACAGCACTCAGGCAAGCCATTCTC2920.10886707404825198No Hit
GTACAGACTTGGCCCCCAGTACCACATCATCCATATAGCTGAAAGCCAAA2900.10812140915751052No Hit
GAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGAATACTAAC2890.1077485767121398No Hit
TCACCATACAGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTAATGA2890.1077485767121398No Hit
GTTTTAGAAAACTTCCTGTCAGTCGACCTATTGATTGGAAAGTATGTCAG2880.10737574426676907No Hit
GTCCAACTGATGATCGGGAAAGAATCCCAGAGGGTTGGGAACAGAAAGGT2860.10663007937602761No Hit
CTCTGGGATCTTGCAGAGCTTGGTGAAAAGCTGTGGAATTCCACTGCATG2850.1062572469306569No Hit
ACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGAGTTGCTCTCATTT2840.10588441448528617No Hit
GTCTTACATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT2810.10476591714917399No Hit
CTCTTTGGAAGGCTGGGATTCTATATAAGAGGGAAACTACACGTAGCGCC2800.10439308470380326No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT2790.10402025225843255No Hit
GTCTTCATGTAAATGAATTTTAGGAAAAGAAGGGGTTTGCCATTCAGGAT2780.10364741981306182No Hit
AGGTTGTACTGCCAACTGGATTCTTCGCGGGACGTCCTTTGTTTACGTCC2770.10327458736769109No Hit
GTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTGCACTTCGCTTC2750.10252892247694964No Hit
CCTCCAAGCTGTGCCTTGGATGGCTTTGGGGCATGGACATTGACCCTTAT2740.10215609003157891No Hit
GGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA2740.10215609003157891No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTGCTCTCATTTTTGCCTTCT2730.10178325758620818No Hit
ATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACAG2710.10103759269546674No Hit
GTATGCATGTATACAAGCGAAACAGGCTTTTACTTTCTCGCCAACTTACA2690.10029192780472529No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)