FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533573
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT44340.8310015686700789No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTT30290.5676823977225234No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25970.4867187807479014No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA21170.39675920633165473No Hit
ATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATAACCCATGAA18650.3495304297631252No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT17550.328914693959402No Hit
GCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAAAG16290.3053003056751372No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTACTCCCTAAATTTCAT14520.27212771260914626No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACA14110.2644436656277585No Hit
CCTATTAGCAATGTCTTCATGTAAATGTATTTTAGGAAAGGAAGGGGTTT13880.26013310268698003No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACCCGACATTATT13820.25900860800677694No Hit
TTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACCCCTTCCTTTC13600.2548854608460323No Hit
GTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCAGG13180.24701399808461072No Hit
GGATCATATTGTACAAAAAATCAAACACTGTTTTAGAAAACTTCCTGTCA12930.24232860358376457No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT11380.21327915767851824No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10840.20315870555669044No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTCCCTATC10320.1934130849949304No Hit
GTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAA9290.1741092596514441No Hit
CTTTAATCCTGAATGGCAAACCCCTTCCTTTCCTAAAATACATTTACATG9200.1724225176311395No Hit
GTTTTATCCTAACAGCACAAAATATTTGCCTTTAGACAAAGGGATTAAAC9170.17186027029103798No Hit
CATGAAGACATTGCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGT9010.1688616178104964No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAGTCTTCTTTTTTCATTTAC9000.16867420203046254No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG8840.165675549549921No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC8780.16455105486971794No Hit
GTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT8670.16248948128934562No Hit
GCTTTATTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACCCCTT8480.1589285814687025No Hit
GTGTAGTTTCCCTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAA8480.1589285814687025No Hit
CTGACATACTTTCCAATCAATAGGTCGATTGACAGGAAGTTTTCTAAAAC8110.15199419760745014No Hit
CTACATGATCAGGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATAT8060.15105711870728092No Hit
TTCTAAAACAGTGTTTGATTTTTTGTACAATATGATCCTGTGGCAATGTA7620.14281082438579162No Hit
CTCTCACTGTAAATGAAAAAAGAAGACTAAAATTAATTATGCCTGCTAGG7620.14281082438579162No Hit
CTGTAAATGAAAAAAGAAGACTAAAATTAATTATGCCTGCTAGGTTTTAT7450.13962475612521624No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG7260.13606385630457313No Hit
CCATTTACACAATGTGGGTACCCTGCATTAATGCCTTTGTATGCATGTAT7250.1358764405245393No Hit
CTTCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTA7220.13531419318443774No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC7200.13493936162437006No Hit
GAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCA7170.13437711428426852No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGGCAAGAAATATGAAACCACA7150.1340022827242008No Hit
GTATACATGCATACAAAGGCATTAATGCAGGGTACCCACATTGTGTAAAT7130.13362745116413313No Hit
GGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAA6980.1308162144636254No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG6970.13062879868359156No Hit
ATATTTGCCTTTAGACAAAGGGATTAAACCTTATTATCCTGATCATGTAG6950.13025396712352388No Hit
CCTTTAGACAAAGGGATTAAACCTTATTATCCTGATCATGTAGTTAATCA6770.1268804830829146No Hit
GTCCCTATCTTTAATCCTGAATGGCAAACCCCTTCCTTTCCTAAAATACA6630.12425666216244076No Hit
GATCAGGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTG6510.12200767280203459No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA6480.12144542546193304No Hit
GCACAAAATATTTGCCTTTAGACAAAGGGATTAAACCTTATTATCCTGAT6470.1212580096818992No Hit
CCCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG6450.1208831781218315No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6420.12032093078172997No Hit
GGCAAATATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAATTAATTT6250.11713486252115456No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6020.11282429958037607No Hit
CTATACAATTCACCCAGTTAAACTATGCAAGTCATTTCTTAAAATATATT6020.11282429958037607No Hit
ACGTGTAGTTTCCCTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTA5970.11188722068020682No Hit
GTACCCACATTGTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTC5930.11113755756007145No Hit
ATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAGTCTT5910.11076272600000375No Hit
GTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAAC5880.11020047865990219No Hit
ATATAGAGCTGAGGCTGTATCTAGTAGATCCCGGACGGAAGGAAAGAAGT5780.10832632085956374No Hit
CTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACCCCTTCCTTTCCT5660.10607733149915757No Hit
TATCCTAACAGCACAAAATATTTGCCTTTAGACAAAGGGATTAAACCTTA5400.10120452121827753No Hit
ATTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACCCCTTCCTTT5400.10120452121827753No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)