FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490799
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTATTCTTTAAATTTCAT41820.852079975713072No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT31020.6320306276092656No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT23820.48533106220672817No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23660.4820710718644496No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT21520.4384687010364732No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC18340.37367639298368577No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA17930.36532266773159683No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC17070.3478002196418493No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACCACCGTG16960.34555897628153276No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC15890.3237577908675445No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTATT15140.3084765861381136No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT14930.3041978488138729No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA14720.2999191114896322No Hit
ACCCATAACATCCAATGACATAGCCCATGAAATTTAAAGAATAACCCCAT14610.2976778681293157No Hit
GACATAGCCCATGAAATTTAAAGAATAACCCCATCTTTTAGTTTTGTTAG14510.2956403741653915No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG13390.27282044176944126No Hit
CTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA13370.2724129429766564No Hit
GTCCCACGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC13130.2675229574632385No Hit
GACCCATAACATCCAATGACATAGCCCATGAAATTTAAAGAATAACCCCA13120.2673192080668461No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG12900.26283672134621305No Hit
CTTTTACACAATGTGGTTATCCTGCGTTAATGCCCTTATATGCCTGTATT12890.2626329719498206No Hit
TTGACAGACTTTCCAATCAATCGGCCTGTTAACAGGAAGTTTTCTAAAAC12570.2561129912652634No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG12400.25264925152659234No Hit
GGGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTATTCTTTAA12220.24898176239152892No Hit
ATAACATCCAATGACATAGCCCATGAAATTTAAAGAATAACCCCATCTTT12060.2457217720492503No Hit
GGCATATAAGGGCATTAACGCAGGATAACCACATTGTGTAAAAGGGGCAG12060.2457217720492503No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA11980.24409177687811098No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA11860.24164678412140206No Hit
GGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTATTCTTTAAA11800.24042428774304755No Hit
CTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA11780.24001678895026274No Hit
GAATAACCCCATCTTTTAGTTTTGTTAGGGTTTAAATGTATACCCAAAGA11590.23614555041880692No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC11500.2343118058512752No Hit
ATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATGAA11380.23186681309456622No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA11160.22738432637393313No Hit
CAATGACATAGCCCATGAAATTTAAAGAATAACCCCATCTTTTAGTTTTG10940.22290183965330002No Hit
CATCCAATGACATAGCCCATGAAATTTAAAGAATAACCCCATCTTTTAGT10570.21536311198678076No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT10110.20599063975272974No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT9860.2008969048429194No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG9770.19906316027538767No Hit
AGATCACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTA9590.19539567114032425No Hit
CTTTCCAATCAATCGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTG9590.19539567114032425No Hit
GCGTGGGACCATGCAGAACCTGCACGACTACTGTTCAAGGAACCTCTATG9550.19458067355475459No Hit
GGGTTATTCTTTAAATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT9480.19315442778000771No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG9450.19254317959083045No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC9260.1886719410593746No Hit
ACAATACGTTGACAGACTTTCCAATCAATCGGCCTGTTAACAGGAAGTTT9230.1880606928701974No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT9220.18785694347380497No Hit
ACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGG8730.1778732230505767No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA8720.1776694736541843No Hit
ACATAGAGGTTCCTTGAACAGTAGTCGTGCAGGTTCTGCATGGTCCCACG8390.17094574357323467No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC8370.17053824478044985No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8320.16951949779848777No Hit
GAATACAGGCATATAAGGGCATTAACGCAGGATAACCACATTGTGTAAAA8320.16951949779848777No Hit
ACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTT7970.1623882689247533No Hit
TCCAATGACATAGCCCATGAAATTTAAAGAATAACCCCATCTTTTAGTTT7950.1619807701319685No Hit
GTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTATTCTTTAAAT7910.16116577254639883No Hit
TTTAAACCCTAACAAAACTAAAAGATGGGGTTATTCTTTAAATTTCATGG7860.16014702556443677No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT7850.15994327616804435No Hit
ATACGTTGACAGACTTTCCAATCAATCGGCCTGTTAACAGGAAGTTTTCT7540.15362704487987955No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7410.15097830272677817No Hit
ACCCAAAAGACCCACAATACGTTGACAGACTTTCCAATCAATCGGCCTGT7360.1499595557448161No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA7350.1497558063484237No Hit
CCCATAACATCCAATGACATAGCCCATGAAATTTAAAGAATAACCCCATC7320.14914455815924646No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC7090.14445832204222095No Hit
GTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAAAC7090.14445832204222095No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC7030.14323582566386647No Hit
CATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCA6980.14221707868190442No Hit
GTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA6980.14221707868190442No Hit
CTTTTAGTTTTGTTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATT6950.14160583049272715No Hit
ACTAAAAGATGGGGTTATTCTTTAAATTTCATGGGCTATGTCATTGGATG6950.14160583049272715No Hit
GTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGT6930.14119833169994234No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG6900.1405870835107651No Hit
GCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATG6880.14017958471798028No Hit
CAATACGTTGACAGACTTTCCAATCAATCGGCCTGTTAACAGGAAGTTTT6820.1389570883396258No Hit
CAGTAAACCCTGTTCCGACCACTGCCTCTCCCATATCGTCAATCTTCTCG6790.13834584015044857No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC6760.1377345919612713No Hit
GTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGC6740.13732709316848649No Hit
ATTCTCTGACATACTTTCCAATCAATAGGTCGATTGACAGGAAGTTTTCT6690.1363083461865244No Hit
GTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAAT6660.1356970979973472No Hit
TCCTTGAACAGTAGTCGTGCAGGTTCTGCATGGTCCCACGCTGGTGGTTG6570.13386335342981545No Hit
GGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCC6490.13223335825867616No Hit
GTTCAAGGAACCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGA6480.13202960886228374No Hit
CATATAAGGGCATTAACGCAGGATAACCACATTGTGTAAAAGGGGCAGCA6410.13060336308753684No Hit
GAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC6360.12958461610557478No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6340.12917711731278994No Hit
TTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCT6240.12713962334886583No Hit
TTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC6190.12612087636690378No Hit
ATGTGGTTATCCTGCGTTAATGCCCTTATATGCCTGTATTCAATCTAAGC6110.12449088119576446No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGC6080.1238796330065872No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC6070.1236758836101948No Hit
CTAAAAGATGGGGTTATTCTTTAAATTTCATGGGCTATGTCATTGGATGT6060.12347213421380239No Hit
GGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG5970.12163838964627068No Hit
GCCCATGACCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGC5960.12143464024987827No Hit
GATACATAGAGGTTCCTTGAACAGTAGTCGTGCAGGTTCTGCATGGTCCC5950.12123089085348585No Hit
CCTGTTAACAGGCCGATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCT5930.12082339206070103No Hit
GATTGAATACAGGCATATAAGGGCATTAACGCAGGATAACCACATTGTGT5910.12041589326791619No Hit
GCGCCGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGT5830.11878589809677688No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC5830.11878589809677688No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA5760.11735965232203001No Hit
TTATATGCCTGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTA5650.11511840896171344No Hit
ATCTTTTAGTTTTGTTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAA5640.11491465956532103No Hit
TCTCCTGGCTCAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTT5610.1143034113761438No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5550.11308091499778931No Hit
CTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTG5500.11206216801582726No Hit
TTCTAAAACATTCTTTGATTTTCTGTATGATGTGATCTTGTGGCAATGAC5450.11104342103386519No Hit
CAATCGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT5420.11043217284468795No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5390.1098209246555107No Hit
TCTTTTAGTTTTGTTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAAT5380.1096171752591183No Hit
TTCTTTGATTTTCTGTATGATGTGATCTTGTGGCAATGACCCATAACATC5320.10839467888076383No Hit
CAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGATTGAA5310.1081909294843714No Hit
CCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGAAGATGATAAAA5280.10757968129519416No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA5260.10717218250240934No Hit
ATTTAAAGAATAACCCCATCTTTTAGTTTTGTTAGGGTTTAAATGTATAC5260.10717218250240934No Hit
CCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACCA5240.10676468370962452No Hit
GTGGTCCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG5210.10615343552044726No Hit
GGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGA5190.10574593672766244No Hit
CTTATATGCCTGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTT5190.10574593672766244No Hit
GCCCATGAAATTTAAAGAATAACCCCATCTTTTAGTTTTGTTAGGGTTTA5180.10554218733127003No Hit
ACATTCTTTGATTTTCTGTATGATGTGATCTTGTGGCAATGACCCATAAC5170.10533843793487763No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC5160.1051346885384852No Hit
TTTCTGTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACAT5110.10411594155652314No Hit
CCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACTCAAGATG5050.10289344517816865No Hit
GCTTAGATTGAATACAGGCATATAAGGGCATTAACGCAGGATAACCACAT5020.10228219698899142No Hit
CTCCCATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCGGAACATG4980.10146719940342178No Hit
ATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATGAAATTTA4930.1004484524214597No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)