FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490799
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTATTCTTTAAATTTCAT38540.7852501736963604No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT32530.6627967864645201No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23460.4779960839366013No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT22680.4621036310179931No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT20430.4162600168297001No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACCACCGTG18200.37082390143419197No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA18100.36878640747026786No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC17250.35146770877691275No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTATT17190.35024521239855827No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC16430.33476025827273487No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC15470.3152003162190632No Hit
CTTTTACACAATGTGGTTATCCTGCGTTAATGCCCTTATATGCCTGTATT13840.28198916460709983No Hit
CTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA13640.2779141766792516No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT13460.2742466875441882No Hit
ACCCATAACATCCAATGACATAGCCCATGAAATTTAAAGAATAACCCCAT13430.2736354393550109No Hit
CTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA12380.25224175273380756No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA12310.25081550695906063No Hit
GACATAGCCCATGAAATTTAAAGAATAACCCCATCTTTTAGTTTTGTTAG12210.2487780129951365No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG11810.24062803713943998No Hit
GTCCCACGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC11800.24042428774304755No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA11780.24001678895026274No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA11660.23757179619355376No Hit
GACCCATAACATCCAATGACATAGCCCATGAAATTTAAAGAATAACCCCA11660.23757179619355376No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC11470.23370055766209794No Hit
GGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTATTCTTTAAA11230.22881057214868No Hit
GAATAACCCCATCTTTTAGTTTTGTTAGGGTTTAAATGTATACCCAAAGA11190.22799557456311034No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA11100.22616182999557863No Hit
GGGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTATTCTTTAA11090.22595808059918623No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG10870.22147559387855312No Hit
TTGACAGACTTTCCAATCAATCGGCCTGTTAACAGGAAGTTTTCTAAAAC10510.21414061560842626No Hit
ATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATGAA10500.21393686621203387No Hit
ATAACATCCAATGACATAGCCCATGAAATTTAAAGAATAACCCCATCTTT10470.2133256180228566No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT10010.20395314578880558No Hit
CTTTCCAATCAATCGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTG9990.20354564699602076No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG9940.20252690001405868No Hit
AGATCACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTA9870.20110065423931184No Hit
CATCCAATGACATAGCCCATGAAATTTAAAGAATAACCCCATCTTTTAGT9760.19885941087899525No Hit
GGCATATAAGGGCATTAACGCAGGATAACCACATTGTGTAAAAGGGGCAG9700.19763691450064078No Hit
CAATGACATAGCCCATGAAATTTAAAGAATAACCCCATCTTTTAGTTTTG9280.18907943985215944No Hit
ACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGG9120.18581944950988083No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA9110.1856157001134884No Hit
GGGTTATTCTTTAAATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT8920.18174446158203256No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG8790.1790957194289312No Hit
GTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTATTCTTTAAAT8750.17828072184336152No Hit
GCGTGGGACCATGCAGAACCTGCACGACTACTGTTCAAGGAACCTCTATG8490.1729832375371588No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC8210.16727825443817124No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC8060.16422201349228502No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT8010.16320326651032296No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT7960.16218451952836088No Hit
ACAATACGTTGACAGACTTTCCAATCAATCGGCCTGTTAACAGGAAGTTT7820.1593320279788671No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT7780.15851703039329745No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7670.15627578703298092No Hit
GTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAAAC7570.1542382930690568No Hit
GCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATG7470.15220079910513265No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7400.15077455333038578No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC7310.14894080876285404No Hit
GTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAAT7290.1485333099700692No Hit
ATACGTTGACAGACTTTCCAATCAATCGGCCTGTTAACAGGAAGTTTTCT7210.1469033147989299No Hit
CAGTAAACCCTGTTCCGACCACTGCCTCTCCCATATCGTCAATCTTCTCG7160.14588456781696785No Hit
GTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA7150.14568081842057543No Hit
CATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCA7150.14568081842057543No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA7140.14547706902418303No Hit
GTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGT7060.1438470738530437No Hit
GAATACAGGCATATAAGGGCATTAACGCAGGATAACCACATTGTGTAAAA7050.14364332445665132No Hit
TTTAAACCCTAACAAAACTAAAAGATGGGGTTATTCTTTAAATTTCATGG7010.14282832687108166No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC7010.14282832687108166No Hit
TCCAATGACATAGCCCATGAAATTTAAAGAATAACCCCATCTTTTAGTTT7000.14262457747468923No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC6980.14221707868190442No Hit
ACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTT6930.14119833169994234No Hit
GTTCAAGGAACCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGA6630.13508584980816996No Hit
ACATAGAGGTTCCTTGAACAGTAGTCGTGCAGGTTCTGCATGGTCCCACG6600.1344746016189927No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6560.13365960403342306No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG6560.13365960403342306No Hit
CTTTTAGTTTTGTTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATT6550.13345585463703064No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6490.13223335825867616No Hit
CAATACGTTGACAGACTTTCCAATCAATCGGCCTGTTAACAGGAAGTTTT6420.13080711248392926No Hit
ATTCTCTGACATACTTTCCAATCAATAGGTCGATTGACAGGAAGTTTTCT6240.12713962334886583No Hit
TTATATGCCTGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTA6060.12347213421380239No Hit
GTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGC5980.12184213904266308No Hit
ACTAAAAGATGGGGTTATTCTTTAAATTTCATGGGCTATGTCATTGGATG5950.12123089085348585No Hit
ATGTGGTTATCCTGCGTTAATGCCCTTATATGCCTGTATTCAATCTAAGC5930.12082339206070103No Hit
ACCCAAAAGACCCACAATACGTTGACAGACTTTCCAATCAATCGGCCTGT5890.12000839447513137No Hit
ATCTTTTAGTTTTGTTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAA5820.11858214870038447No Hit
TCTTTTAGTTTTGTTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAAT5790.11797090051120723No Hit
CCCATAACATCCAATGACATAGCCCATGAAATTTAAAGAATAACCCCATC5780.11776715111481482No Hit
GGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGA5760.11735965232203001No Hit
TTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCT5740.11695215352924516No Hit
TTCTAAAACATTCTTTGATTTTCTGTATGATGTGATCTTGTGGCAATGAC5720.11654465473646033No Hit
TTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC5700.11613715594367552No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5630.11471091016892862No Hit
CCTGTTAACAGGCCGATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCT5620.11450716077253621No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA5600.1140996619797514No Hit
GAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC5590.11389591258335897No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC5520.11246966680861209No Hit
GGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG5490.11185841861943482No Hit
GGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCC5480.11165466922304242No Hit
TTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGTT5480.11165466922304242No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGC5460.1112471704302576No Hit
GATACATAGAGGTTCCTTGAACAGTAGTCGTGCAGGTTCTGCATGGTCCC5430.11063592224108036No Hit
GCCCATGACCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGC5410.11022842344829555No Hit
CTCCCATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCGGAACATG5380.1096171752591183No Hit
CTTATATGCCTGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTT5340.10880217767354865No Hit
TCCTTGAACAGTAGTCGTGCAGGTTCTGCATGGTCCCACGCTGGTGGTTG5320.10839467888076383No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC5290.10778343069158658No Hit
TTCTTTGATTTTCTGTATGATGTGATCTTGTGGCAATGACCCATAACATC5270.10737593189880175No Hit
CCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACCA5250.10696843310601693No Hit
ATTTAAAGAATAACCCCATCTTTTAGTTTTGTTAGGGTTTAAATGTATAC5230.10656093431323209No Hit
TTTCTGTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACAT5180.10554218733127003No Hit
GATTGAATACAGGCATATAAGGGCATTAACGCAGGATAACCACATTGTGT5160.1051346885384852No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC5100.10391219216013073No Hit
CAATCGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT5090.10370844276373832No Hit
ACCCTGTTCCGACCACTGCCTCTCCCATATCGTCAATCTTCTCGAGGATT5090.10370844276373832No Hit
CCTTTTACACAATGTGGTTATCCTGCGTTAATGCCCTTATATGCCTGTAT5080.1035046933673459No Hit
GTTCAGGAACAGTAAACCCTGTTCCGACCACTGCCTCTCCCATATCGTCA5060.10309719457456108No Hit
CTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTG5040.10268969578177624No Hit
CTAAAAGATGGGGTTATTCTTTAAATTTCATGGGCTATGTCATTGGATGT5030.10248594638538383No Hit
CATATAAGGGCATTAACGCAGGATAACCACATTGTGTAAAAGGGGCAGCA5020.10228219698899142No Hit
GGGGACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATC5010.10207844759259901No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA5000.10187469819620659No Hit
CCCTAACAAAACTAAAAGATGGGGTTATTCTTTAAATTTCATGGGCTATG4940.10065220181785212No Hit
CCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACTCAAGATG4920.10024470302506729No Hit
ATTTAAACCCTAACAAAACTAAAAGATGGGGTTATTCTTTAAATTTCATG4910.10004095362867488No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)