FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590085
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29560.500944779141988No Hit
GCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA25690.4353610073125058No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC22020.3731665776964336No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT17840.302329325436166No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT16750.2838574103730819No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT16580.28097646949168337No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTACTCTCTGAATTTTAT16080.27250311395815857No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG14120.23928756026674122No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG12420.2104781514527568No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTT12380.20980028301007483No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGACACACT11730.19878492081649252No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC11220.1901420981722972No Hit
CTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTT11130.18861689417626273No Hit
TTCTATTACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA11060.18743062440156927No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTTTGT10360.1755679266546345No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT10270.17404272265860002No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA10260.17387325554792954No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9690.16421363023971122No Hit
TAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT9600.16268842624367674No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTTTCCATGC9570.16218002491166528No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT9550.1618410906903243No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT9290.15743494581289136No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9230.15641814314886837No Hit
CTCTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGT9230.15641814314886837No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA8910.15099519560741248No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT8800.14913105739003701No Hit
CACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGT8720.14777532050467304No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT8720.14777532050467304No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTG8630.1462501165086386No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC8390.14218290585254667No Hit
ATCTAAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG8010.1357431556470678No Hit
GTATCGGGATGCCTTAGAATCTCCTGAGCATTGTTCACCTCACCATACTG8010.1357431556470678No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT7990.13540422142572678No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7970.13506528720438582No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT7940.13455688587237433No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC7730.13099807654829387No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT7690.1303202081056119No Hit
CTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACCGTGGA7550.12794766855622494No Hit
ATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA7480.12676139878153148No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAATCTCCTCC7380.12506672767482652No Hit
GCCTAGCAGCCATGGAAATGATGTATATTTGCGGGAGAGGACAACAGAGT7140.1209995170187346No Hit
GTTTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTA7090.1201521814653821No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG7080.11998271435471161No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAATCTTCTTTTCTCA6910.11710177347331316No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCG6910.11710177347331316No Hit
GTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTATCC6890.11676283925197217No Hit
GTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTTTGTATGATGTGTT6840.11591550369861969No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGG6780.1148987010345967No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAATCT6730.11405136548124423No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC6680.11320402992789173No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG6630.11235669437453927No Hit
GATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGAGTAACTCC6600.11184829304252777No Hit
CTATTACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAG6580.11150935882118679No Hit
ACATCGACCCTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGTTT6540.11083149037850479No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA6520.1104925561571638No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT6480.1098146877144818No Hit
CTCCGAAAGACACCAAATATTCTATTACTGTTTCTCTTCCAAAAGTGAGA6460.10947575349314081No Hit
CCTACAAACTGTTCACATTTTTTGATAATGTCTTGGTGTAAATGTATATT6380.10812001660777686No Hit
TCCCGATACAGTGCTGAGGCGGTATCTAGAAGATGTCGTACTGAAGGAAA6350.10761161527576535No Hit
ATCTAGAAGATGTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA6320.10710321394375387No Hit
CTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA6300.10676427972241287No Hit
CAGTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTAT6270.10625587839040139No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC6220.1054085428370489No Hit
GGCATAATCAATTGCAATCTTCTTTTCTCATTAACTGTGAGTGGGCCTAC6170.10456120728369642No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6150.10422227306235543No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6140.10405280595168494No Hit
CTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCTTG6080.10303600328766195No Hit
TCCCGATACAGTGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAA6050.10252760195565046No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6010.10184973351296848No Hit
ACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTG6000.10168026640229796No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA5960.10100239795961599No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5920.10032452951693399No Hit
CTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC5920.10032452951693399No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)