FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590085
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29270.49603023293254367No Hit
GCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA25290.428582322885686No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC21710.3679130972656482No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT17900.30334612810018896No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT16970.28758568680783275No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT15760.2670801664167027No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTACTCTCTGAATTTTAT15430.2614877517645763No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC13710.23233940872925085No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG13370.22657752696645397No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG12130.20556360524331244No Hit
TTCTATTACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA11870.20115746036587948No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGACACACT11790.19980172348051553No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTT11110.18827795995492175No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT10940.1853970190735233No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT10840.18370234796681834No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA10820.18336341374547735No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT10490.17777099909335098No Hit
CTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTT9840.16675563689976866No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTTTGT9660.16370522890769973No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9640.16336629468635874No Hit
CTCTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGT9220.1562486760381979No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC9030.15302880093545845No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT9020.15285933382478795No Hit
GTATCGGGATGCCTTAGAATCTCCTGAGCATTGTTCACCTCACCATACTG8920.151164662718083No Hit
TAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT8790.14896159027936653No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA8680.14709745206199107No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT8600.1457417151766271No Hit
CTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACCGTGGA8490.14387757695925163No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8430.14286077429522867No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG8300.1406577018565122No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT8210.13913249786047774No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTG8030.13608208986840878No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG7890.13370955031902185No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7880.13354008320835134No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT7840.13286221476566934No Hit
ATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA7760.1315064778803054No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC7640.12947287255225942No Hit
ATCTAAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG7620.12913393833091844No Hit
GTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTTTGTATGATGTGTT7320.12404992501080353No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAATCTCCTCC7170.12150791835074608No Hit
GTTTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTA7160.12133845124007558No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA7040.11930484591202963No Hit
CACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGT7010.11879644458001815No Hit
GTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTATCC6980.11828804324800664No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT6890.11676283925197217No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA6860.11625443791996068No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA6800.11523763525593769No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAGAGATGGGGTTACT6780.1148987010345967No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6730.11405136548124423No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6680.11320402992789173No Hit
GCCTAGCAGCCATGGAAATGATGTATATTTGCGGGAGAGGACAACAGAGT6640.11252616148520976No Hit
CTCCGAAAGACACCAAATATTCTATTACTGTTTCTCTTCCAAAAGTGAGA6640.11252616148520976No Hit
ACATTGACCCTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGTTT6610.11201776015319827No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6560.11117042459984577No Hit
ACATCGACCCTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGTTT6550.11100095748917528No Hit
CTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA6510.11032308904649331No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG6490.10998415482515231No Hit
CTATTACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAG6450.10930628638247034No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTTTCCATGC6340.10744214816509486No Hit
GTTATGTCAACACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTT6320.10710321394375387No Hit
GGCATAATCAATTGCAATCTTCTTTTCTCATTAACTGTGAGTGGGCCTAC6250.10591694416906038No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCG6190.10490014150503742No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGG6170.10456120728369642No Hit
GTATATGGTGACCCACAAAATGAAGCGCTATGTGTTGTTTCTCTCTTATA6160.10439174017302592No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAATCTTCTTTTCTCA6150.10422227306235543No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAATCT6120.10371387173034392No Hit
CAGTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTAT6060.10269706906632095No Hit
CTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGTTTTTGCCTTCT6050.10252760195565046No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6010.10184973351296848No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC5980.10134133218095698No Hit
CCTACAAACTGTTCACATTTTTTGATAATGTCTTGGTGTAAATGTATATT5920.10032452951693399No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)