FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002488295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20742
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG2731.316170089673127No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA2231.0751132966927006No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT2121.0220808022370071No Hit
GCGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT2030.9786905795005303No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG1700.819593096133449No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC1470.708706971362453No Hit
GGATAACCACATTGCGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT1440.6942435637836275No Hit
GTGAAAGCCTGCTTAGATTGAATACATGCATACAAGGGCATTAAAGCAGG1430.6894224279240189No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCT1110.5351460804165461No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGATACTCTTTACATTTCAT850.4097965480667245No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG790.38086973290907333No Hit
GGTTTGGTACAGCAACAGGAGGGATACATAGAGGTTCCTTGAGCAGGAGT770.37122746118985633No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC760.3664063253302478No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA740.35676405361103075No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCT710.34230064603220517No Hit
GCACGAGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG700.33747951017259664No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAATCTTTGATTTTTTGT700.33747951017259664No Hit
GATCCTGTGGCAATGACCCATAACATCCAATGACATAGCCCATGAAATGT690.33265837431298817No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC650.31337383087455406No Hit
GATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCCCATGA640.30855269501494553No Hit
GCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAA630.303731559155337No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG630.303731559155337No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG620.2989104232957285No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC610.29408928743611995No Hit
ACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA590.2844470157169029No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC590.2844470157169029No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC580.27962587985729437No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG580.27962587985729437No Hit
GGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACCAGCAC570.2748047439976859No Hit
CTTTCACGCAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT570.2748047439976859No Hit
GCTTAGATTGAATACATGCATACAAGGGCATTAAAGCAGGATAACCACAT550.2651624722784688No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC540.26034133641886026No Hit
ATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATGAAATGTA520.2506990646996432No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC520.2506990646996432No Hit
CCTCCTGTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGT510.2458779288400347No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA510.2458779288400347No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC510.2458779288400347No Hit
ATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATGAA500.2410567929804262No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC490.23623565712081765No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC490.23623565712081765No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT480.23141452126120912No Hit
CCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCTTGGGCTTT470.22659338540160062No Hit
GTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTA460.2217722495419921No Hit
AGATCACATCATACAAAAAATCAAAGATTGTTTTAGAAAACTTCCTGTTA460.2217722495419921No Hit
GTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGT450.21695111368238354No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA450.21695111368238354No Hit
GTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGC440.21212997782277507No Hit
CTTTTACGCAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT440.21212997782277507No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN430.2073088419631665No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT430.2073088419631665No Hit
GTTCAGGAACAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCA430.2073088419631665No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG420.20248770610355799No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC420.20248770610355799No Hit
GGTTCTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAG410.19766657024394949No Hit
ATCACGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC410.19766657024394949No Hit
GGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG400.19284543438434096No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG400.19284543438434096No Hit
ATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGATACATAGAGGTTC390.18802429852473243No Hit
GGGATACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT390.18802429852473243No Hit
GAGTATCCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA390.18802429852473243No Hit
CACAAGATCACATCATACAAAAAATCAAAGATTGTTTTAGAAAACTTCCT380.1832031626651239No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG380.1832031626651239No Hit
CTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG370.17838202680551538No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC370.17838202680551538No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT370.17838202680551538No Hit
CAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGATTGAA370.17838202680551538No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA360.17356089094590688No Hit
GCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCTGGCC360.17356089094590688No Hit
CTCTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACA350.16873975508629832No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAAAAA350.16873975508629832No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC350.16873975508629832No Hit
GACATAGCCCATGAAATGTAAAGAGTATCCCCATCTTTTTGTTTTGTTAG340.16391861922668982No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGATACT340.16391861922668982No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG340.16391861922668982No Hit
GGATCACATCATACAAAAAATCAAAGATTGTTTTAGAAAACTTCCTGTTA340.16391861922668982No Hit
GTAAAGAGTATCCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCC340.16391861922668982No Hit
GTCTGTCAACGTATTGTGGGTCTTTTGGGTTTTGCTGCCCCTTTTACGCA330.1590974833670813No Hit
ATGTAAAGAGTATCCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATAC330.1590974833670813No Hit
ACCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACC330.1590974833670813No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGCGTAAAA320.15427634750747277No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAAAAAATCAAAGATTGT320.15427634750747277No Hit
GTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTA310.14945521164786424No Hit
GATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATGAAATGT310.14945521164786424No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGATACTCTTTAC310.14945521164786424No Hit
GAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC310.14945521164786424No Hit
GTTAACAGGAAGTTTTCTAAAACAATCTTTGATTTTTTGTATGATGTGAT300.1446340757882557No Hit
AACGCGCAACATACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGC300.1446340757882557No Hit
GCCCATGACCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC300.1446340757882557No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG300.1446340757882557No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA300.1446340757882557No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGCGTAAAAGGGGC290.13981293992864718No Hit
CTCACCAACCTCCTGTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTC290.13981293992864718No Hit
CATTAAAGCAGGATAACCACATTGCGTAAAAGGGGCAGCAAAACCCAAAA280.13499180406903866No Hit
GCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAG280.13499180406903866No Hit
GCCCAAGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTT280.13499180406903866No Hit
CCTCCAGCTTATAGACCTCCAAATGCCCCTATCTTATCAACACTTCCGGA280.13499180406903866No Hit
GTTTGGTACAGCAACAGGAGGGATACATAGAGGTTCCTTGAGCAGGAGTC280.13499180406903866No Hit
GATGGGGATACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGG280.13499180406903866No Hit
CACTTGGCACAGACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGT280.13499180406903866No Hit
CCCATGAAATGTAAAGAGTATCCCCATCTTTTTGTTTTGTTAGGGTTTAA280.13499180406903866No Hit
ACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCC280.13499180406903866No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAATCTTTG270.13017066820943013No Hit
ACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCGAGGATT270.13017066820943013No Hit
GACCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTATCCCCA270.13017066820943013No Hit
CCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAA260.1253495323498216No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGGAAAATTCCTATGGGAG260.1253495323498216No Hit
GCGTGAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT260.1253495323498216No Hit
CATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTTTGGCTTTCAGTT260.1253495323498216No Hit
GCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTATCAAGGTATGT260.1253495323498216No Hit
GCCCATGAAATGTAAAGAGTATCCCCATCTTTTTGTTTTGTTAGGGTTTA250.1205283964902131No Hit
CTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTG250.1205283964902131No Hit
GTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGCAG250.1205283964902131No Hit
CCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACTCAAGATG250.1205283964902131No Hit
ATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG250.1205283964902131No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT250.1205283964902131No Hit
GTCCCCAACCTCCAATCACTCACCAACCTCCTGTCCTCCAACTTGTCCTG250.1205283964902131No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA250.1205283964902131No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA240.11570726063060456No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT240.11570726063060456No Hit
ATCTTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCT240.11570726063060456No Hit
GTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCGAG240.11570726063060456No Hit
TTTAAACCCTAACAAAACAAAAAGATGGGGATACTCTTTACATTTCATGG240.11570726063060456No Hit
ATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCTGGCCGTTGC240.11570726063060456No Hit
ACCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTATCCCCAT240.11570726063060456No Hit
AGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAATTT230.11088612477099605No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA230.11088612477099605No Hit
CTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA230.11088612477099605No Hit
GCACAGACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTT230.11088612477099605No Hit
CCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTATCCCCATC230.11088612477099605No Hit
GCAACAGGAGGGATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTC230.11088612477099605No Hit
CAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTT230.11088612477099605No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT230.11088612477099605No Hit
CTCAAGATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAAC230.11088612477099605No Hit
CCCCAACCTCCAATCACTCACCAACCTCCTGTCCTCCAACTTGTCCTGGT220.10606498891138753No Hit
GTCTCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC220.10606498891138753No Hit
CCTCTAATTCCAGGATCTTCAACCACCAGCACGGGACCATGCAGAACCTG220.10606498891138753No Hit
ACCCAAAAGACCCACAATACGTTGACAGACTTTCCAATCAATAGGCCTGT220.10606498891138753No Hit
GTATACATTTAAACCCTAACAAAACAAAAAGATGGGGATACTCTTTACAT210.10124385305177899No Hit
CCCATAGGAATTTTCCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG210.10124385305177899No Hit
GCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAA210.10124385305177899No Hit
GAACTACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC210.10124385305177899No Hit
CCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAG210.10124385305177899No Hit
CCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGACAAAAGA210.10124385305177899No Hit
GCGATAACCAGGACAAGTTGGAGGACAGGAGGTTGGTGAGTGATTGGAGG210.10124385305177899No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC210.10124385305177899No Hit
CAAGATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTG210.10124385305177899No Hit
CAATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCC210.10124385305177899No Hit
ACCTCCAATCACTCACCAACCTCCTGTCCTCCAACTTGTCCTGGTTATCG210.10124385305177899No Hit
ATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACCTG210.10124385305177899No Hit
GTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATC210.10124385305177899No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG210.10124385305177899No Hit
ATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTATCCCCATCTTT210.10124385305177899No Hit
AGACCCACAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGG210.10124385305177899No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)