FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439504
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG37080.8436783282973533No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT36450.8293439877680294No Hit
GTATAATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC35320.8036331865011468No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT32140.7312788962102734No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA26400.6006771269430995No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGAGTAAAA25570.581792202118752No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT24180.5501656412683389No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23930.5444774108995595No Hit
ACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAA22080.5023845061705923No Hit
GAGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT20500.4664348902399068No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC20160.4586988969383669No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA19570.4452746732680476No Hit
CTGTAACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA17310.39385307073428233No Hit
GGATAACCACATTGAGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT17250.3924878954457752No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC16840.38315919764097706No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC15950.3629090975281226No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA15690.3569933379445921No Hit
ACATTATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGG15670.35653827951508976No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG14190.32286395573191595No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT14030.3192234882958972No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG13970.31785831300739015No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG13960.317630783792639No Hit
ACCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCAT13870.31558302085987844No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC13570.3087571444173432No Hit
GACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAG13500.307164439914085No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA13450.3060267938403291No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGAGTAAAAGGGGC13380.3044340893370709No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC13170.29965597582729625No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG13120.29851832975354037No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC13000.2957879791765263No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC12620.28714186901598165No Hit
GGGTTACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT12520.2848665768684699No Hit
GTTATGGGTCATTGCCACAAGATCACATTATACAGAAAATCAAAGAATGT12270.27917834649969053No Hit
ATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATAC12240.27849575885543704No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC11450.2605209508900943No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT11310.2573355418835778No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATTATACAGAAA11300.2571080126688267No Hit
ATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATGAA11290.2568804834540755No Hit
ACCACATTGAGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTT11240.2557428373803196No Hit
TCCTAGCTCAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTC11080.25210236994430085No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA11000.2502821362262915No Hit
GAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAATTTTCCGAAAG10900.2480068440787797No Hit
CATTAAAGCAGGATAACCACATTGAGTAAAAGGGGCAGCAAAACCCAAAA10850.24686919800502383No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCAATCTTCTCG10670.24277367213950274No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGGAAAATTCCTATGGGAG10480.2384506170592304No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT10480.2384506170592304No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATAATGTGAT10460.23799555862972807No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAC10370.2359477956969675No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG10370.2359477956969675No Hit
CTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA10320.23481014962321162No Hit
ACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA10150.23094215297244167No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT10090.22957697768393465No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATTATACA10010.22775674396592524No Hit
GTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGT10000.22752921475117405No Hit
CCTGACAACGGGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGT9990.22730168553642288No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC9850.22411627652990643No Hit
GTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCC9770.22229604281189702No Hit
TAACCACATTGAGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG9730.22138592595289233No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC9510.21638028322836655No Hit
GACCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCA9330.21228475736284538No Hit
CAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTG9230.21000946521533365No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG9210.20955440678583132No Hit
GACCTGGCCGTTGCCTGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC9120.20750664385307074No Hit
ACAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTT9010.20500382249080784No Hit
GCTTAGATTGAATACATGCATACAAGGGCATTAAAGCAGGATAACCACAT8920.20295605955804727No Hit
GCATACAAGGGCATTAAAGCAGGATAACCACATTGAGTAAAAGGGGCAGC8670.1972678291892679No Hit
GTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGC8610.19590265390076084No Hit
TTTCTGTATAATGTGATCTTGTGGCAATGACCCATAACATCCAATGACAT8490.19317230332374677No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT8440.1920346572499909No Hit
GATTGAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGAGT8320.18930430667297682No Hit
ACCGAAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAA8230.18725654374021625No Hit
ATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTT8190.18634642688121153No Hit
AGTCTAGACTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8160.18566383923695803No Hit
GTTCAGGAACAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCA8090.1840711347336998No Hit
TCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTT8070.18361607630419746No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA8060.1833885470894463No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA8030.18270595944519274No Hit
GAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC8000.18202337180093925No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTG7990.18179584258618806No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA7910.17997560886817868No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT7850.17861043357967163No Hit
AGATCACATTATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTA7850.17861043357967163No Hit
GGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACA7770.17679019986166225No Hit
GGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG7490.17041938184862937No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT7460.16973679420437585No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC7410.16859914813061996No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTAACCAATTTTCTT7160.16291091776184063No Hit
TTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCATGG7150.16268338854708944No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC7140.16245585933233828No Hit
ATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGCCTAT7080.16109068404383123No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG7040.16018056718482654No Hit
ACCCAAAAGACCCACAATACGTTGACAGACTTTCCAATCAATAGGCCTGT6930.1576777458225636No Hit
CAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTT6810.15494739524554954No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA6810.15494739524554954No Hit
GGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCC6660.15153445702428192No Hit
CATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGT6630.1508518693800284No Hit
CAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGATTGAA6610.15039681095052604No Hit
CATTATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGC6510.1481215188030143No Hit
GCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTA6480.1474389311587608No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG6450.14675634351450725No Hit
GTCATTGGATGTTATGGGTCATTGCCACAAGATCACATTATACAGAAAAT6450.14675634351450725No Hit
GTGAAAGCCTGCTTAGATTGAATACATGCATACAAGGGCATTAAAGCAGG6340.14425352215224435No Hit
GCCCATGACCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGC6330.14402599293749319No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA6310.14357093450799083No Hit
ATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCT6240.14197823000473261No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCT6220.14152317157523026No Hit
CTTTTACTCAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT6160.1401579962867232No Hit
TCGTAGGGCTTTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGG6010.1367450580654556No Hit
CTCCCATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATG5990.13628999963595326No Hit
GTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACAT5990.13628999963595326No Hit
CCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATC5980.1360624704212021No Hit
CTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTG5850.13310459062943683No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5830.13264953219993447No Hit
ATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCCCTCCT5710.12991918162292038No Hit
CTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA5620.1278714186901598No Hit
GGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATAAT5610.12764388947540864No Hit
CATTGGATGTTATGGGTCATTGCCACAAGATCACATTATACAGAAAATCA5580.12696130183115512No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCT5550.1262787141869016No Hit
TTATGGGTCATTGCCACAAGATCACATTATACAGAAAATCAAAGAATGTT5550.1262787141869016No Hit
GTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA5540.12605118497215043No Hit
CTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG5330.12127307146237577No Hit
GGTTCTGCATGGTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGAC5300.12059048381812223No Hit
ACCCTGTTCCGACTACTGTCTCTCCCATATCGTCAATCTTCTCGAGGATT5260.11968036695911755No Hit
ATGCATACAAGGGCATTAAAGCAGGATAACCACATTGAGTAAAAGGGGCA5250.11945283774436638No Hit
GGATGTTATGGGTCATTGCCACAAGATCACATTATACAGAAAATCAAAGA5220.11877025010011286No Hit
TTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACA5220.11877025010011286No Hit
CCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATAATGT5210.11854272088536169No Hit
TTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC5210.11854272088536169No Hit
GCAGAGGAGCCCGAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAG5180.11786013324110815No Hit
CAATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCC5140.11695001638210346No Hit
CATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5090.11581237030834758No Hit
AATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATA5080.11558484109359642No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA5080.11558484109359642No Hit
GCCCAAGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTT5060.11512978266409407No Hit
GTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTA5050.11490225344934289No Hit
GCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAA5030.11444719501984055No Hit
TGGTTACAGCGGTAAAAAGGGACTCAAGATGCTGTACAGACTTGGCCCCC5030.11444719501984055No Hit
ATCTTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCT4880.11103425679857294No Hit
GTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACTTT4870.11080672758382176No Hit
GTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAAAC4860.11057919836907058No Hit
GCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCTGGCC4850.11035166915431942No Hit
TGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCCCTCCTG4840.11012413993956822No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTATCAAGGTATGTTGC4810.10944155229531471No Hit
CACTTGGCACAGACCTGGCCGTTGCCTGGCAACGGGGTAAAGGTTCAGGT4800.10921402308056354No Hit
CATACAAGGGCATTAAAGCAGGATAACCACATTGAGTAAAAGGGGCAGCA4740.1078488477920565No Hit
GTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTA4730.10762131857730534No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA4700.1069387309330518No Hit
ATACAAGGGCATTAAAGCAGGATAACCACATTGAGTAAAAGGGGCAGCAA4610.10489096800029125No Hit
TTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACAT4580.10420838035603772No Hit
CCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT4550.10352579271178418No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG4520.10284320506753067No Hit
ATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACCTG4510.10261567585277949No Hit
TGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACC4480.10193308820852598No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC4480.10193308820852598No Hit
ACCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACC4470.1017055589937748No Hit
GCATGGTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGG4430.1007954421347701No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)