FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439504
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC40950.9317321344060577No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG37770.8593778441151844No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT37080.8436783282973533No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT33850.7701863919327242No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA26490.60272488987586No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23740.5401543558192872No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT23520.5351487130947613No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGAGTAAAA21320.4850922858495031No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA21160.4814518184134843No Hit
ACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAA20800.473260766682442No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC19110.4348083293894936No Hit
GGATAACCACATTGAGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT18130.41251046634387856No Hit
ACATTATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGG17990.4093250573373621No Hit
CTGTAACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA17980.40909752812261096No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC17350.394763187593287No Hit
GAGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT17000.3867996650769959No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA16430.37383049983617894No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14550.33105500746295824No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG13900.31626560850413193No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC13850.31512796243037605No Hit
GGGTTACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT13760.31308019949761545No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG13700.31171502420910846No Hit
GACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAG13370.3042065601223197No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT13080.29760821289453565No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC12960.2948778623175216No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG12860.29260257017000985No Hit
ACCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCAT12640.287596927445484No Hit
GTTATGGGTCATTGCCACAAGATCACATTATACAGAAAATCAAAGAATGT12430.28281881393570935No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC12420.2825912847209582No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATTATACAGAAA12320.2803159925734464No Hit
ATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATAC12290.2796334049291929No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC12100.2753103498489206No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA11950.271897411627653No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT11890.2705322363391459No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA11400.2593833048163384No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATAATGTGAT11400.2593833048163384No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT11290.2568804834540755No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG11240.2557428373803196No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATTATACA11160.25392260366231023No Hit
ATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATGAA11150.25369507444755907No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAC10870.2473242564345262No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC10670.24277367213950274No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCAATCTTCTCG10300.2343550911937093No Hit
ACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA10240.23298991590520224No Hit
TCCTAGCTCAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTC10160.23116968218719283No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC10100.22980450689868578No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC9960.22661909789216936No Hit
ACCACATTGAGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTT9860.22434380574465762No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGAGTAAAAGGGGC9850.22411627652990643No Hit
GTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCC9780.22252357202664821No Hit
TTTCTGTATAATGTGATCTTGTGGCAATGACCCATAACATCCAATGACAT9770.22229604281189702No Hit
GTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGT9710.22093086752339003No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT9700.22070333830863884No Hit
CTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA9510.21638028322836655No Hit
CATTAAAGCAGGATAACCACATTGAGTAAAAGGGGCAGCAAAACCCAAAA9470.2154701663693618No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG9160.20841676071207543No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGGAAAATTCCTATGGGAG9090.20682405620881722No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA9040.20568641013506134No Hit
AGATCACATTATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTA9010.20500382249080784No Hit
ACAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTT8980.2043212348465543No Hit
GACCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCA8840.20113582584003786No Hit
GTTCAGGAACAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCA8820.2006807674105355No Hit
GAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAATTTTCCGAAAG8760.19931559212202848No Hit
GGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACA8590.19544759547125853No Hit
GACCTGGCCGTTGCCTGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC8540.19430994939750265No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG8460.19248971567949325No Hit
CAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTG8390.19089701117623503No Hit
GCTTAGATTGAATACATGCATACAAGGGCATTAAAGCAGGATAACCACAT8190.18634642688121153No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTG8150.18543631002220684No Hit
TAACCACATTGAGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG8010.18225090101569041No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT7990.18179584258618806No Hit
GAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC7960.18111325494193456No Hit
CCTGACAACGGGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGT7900.17974807965342748No Hit
GATTGAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGAGT7900.17974807965342748No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC7860.1788379627944228No Hit
ACCGAAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAA7860.1788379627944228No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA7850.17861043357967163No Hit
ATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGCCTAT7810.17770031672066694No Hit
ATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTT7710.1754250245731552No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT7500.17064691106338054No Hit
GTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGC7470.169964323419127No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTAACCAATTTTCTT7370.16768903127161527No Hit
TCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTT7330.16677891441261058No Hit
TTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCATGG7280.1656412683388547No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC7250.1649586806946012No Hit
CTTTTACTCAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT7060.1606356256143289No Hit
CATTATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGC7050.1604080963995777No Hit
AGTCTAGACTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6990.15904292111107066No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA6990.15904292111107066No Hit
GTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACAT6830.15540245367505187No Hit
CTCCCATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATG6770.15403727838654485No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6710.1526721030980378No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT6570.14948669409152135No Hit
GCATACAAGGGCATTAAAGCAGGATAACCACATTGAGTAAAAGGGGCAGC6520.1483490480177655No Hit
GTCATTGGATGTTATGGGTCATTGCCACAAGATCACATTATACAGAAAAT6490.14766646037351194No Hit
CATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGT6480.1474389311587608No Hit
GGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG6350.14448105136699552No Hit
CAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTT6350.14448105136699552No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG6340.14425352215224435No Hit
CTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA6240.14197823000473261No Hit
GCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTA6040.13742764570970914No Hit
TTATGGGTCATTGCCACAAGATCACATTATACAGAAAATCAAAGAATGTT6020.13697258728020678No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC6020.13697258728020678No Hit
TTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC6000.13651752885070445No Hit
ACCCAAAAGACCCACAATACGTTGACAGACTTTCCAATCAATAGGCCTGT5980.1360624704212021No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCT5960.13560741199169973No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCT5920.13469729513269504No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5920.13469729513269504No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA5900.1342422367031927No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5880.13378717827369033No Hit
ATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCT5860.133332119844188No Hit
GGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCC5840.13287706141468564No Hit
GGATGTTATGGGTCATTGCCACAAGATCACATTATACAGAAAATCAAAGA5780.1315118861261786No Hit
CATTGGATGTTATGGGTCATTGCCACAAGATCACATTATACAGAAAATCA5740.1306017692671739No Hit
GTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA5690.12946412319341805No Hit
TCGTAGGGCTTTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGG5680.12923659397866685No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA5660.12878153554916452No Hit
ATCTTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCT5650.12855400633441336No Hit
GTGAAAGCCTGCTTAGATTGAATACATGCATACAAGGGCATTAAAGCAGG5610.12764388947540864No Hit
TTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACA5580.12696130183115512No Hit
CTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTG5540.12605118497215043No Hit
GCCCATGACCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGC5520.12559612654264807No Hit
AATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATA5300.12059048381812223No Hit
CCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATC5300.12059048381812223No Hit
CAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGATTGAA5230.11899777931486404No Hit
GTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTA5230.11899777931486404No Hit
CCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATAATGT5190.11808766245585935No Hit
ACCCTGTTCCGACTACTGTCTCTCCCATATCGTCAATCTTCTCGAGGATT5190.11808766245585935No Hit
GGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATAAT5160.11740507481160581No Hit
CATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5150.11717754559685464No Hit
GCCCAAGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTT5070.11535731187884524No Hit
GTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAAAC5050.11490225344934289No Hit
GGTTCTGCATGGTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGAC5020.11421966580508938No Hit
ATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCCCTCCT4950.11262696130183115No Hit
GCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCTGGCC4930.11217190287232881No Hit
CCTGTTAACAGGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCT4910.11171684444282645No Hit
CTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG4880.11103425679857294No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTATCAAGGTATGTTGC4770.10853143543631003No Hit
GGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCC4730.10762131857730534No Hit
GTTAACAGGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTT4720.10739378936255414No Hit
GCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAA4720.10739378936255414No Hit
TGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCCCTCCTG4650.10580108485929594No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA4630.1053460264297936No Hit
TGGTTACAGCGGTAAAAAGGGACTCAAGATGCTGTACAGACTTGGCCCCC4630.1053460264297936No Hit
CACTTGGCACAGACCTGGCCGTTGCCTGGCAACGGGGTAAAGGTTCAGGT4560.10375332192653537No Hit
TTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACAT4540.10329826349703303No Hit
CTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTC4490.10216061742327714No Hit
GCGCTGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGT4410.10034038370526775No Hit
GTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTA4410.10034038370526775No Hit
TGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACC4400.10011285449051659No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)