FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360695
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15490.4294487031979928No Hit
GCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA14070.3900802617169631No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13010.3606925518790114No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT10540.292213643105671No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGACACACT10180.28223291146259305No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTCTGAATTTCAT9270.2570038398092571No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGTAA8650.2398148019795118No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA8400.2328837383384854No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG8260.22900234269951067No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC8240.22844785760822856No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT8120.2251209470605359No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7960.22068506633027904No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT7960.22068506633027904No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT7850.21763539832822745No Hit
CTTTCCAATCAATAGGCCTGTTGACAGGAAGTTTTCTAAAACATTCTTTG7650.2120905474154064No Hit
GTTATGGGTCATTGCCACAAGAACACATCACACAAAAAATCAAAGAATGT7400.20515948377438No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA7210.1998918754072No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC7210.1998918754072No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTGTT7080.1962877223138663No Hit
GTATGCATGTGTTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA6850.18991114376412205No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC6410.17771247175591565No Hit
CTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTT6360.1763262590277104No Hit
CTCTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGT6230.17272210593437667No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6080.1685634677497609No Hit
TTCTATTACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6040.16745449756719666No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5990.1660682848389914No Hit
CAATAGGCCTGTTGACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGT5970.16551379974770927No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC5940.16468207211078612No Hit
ACCCATAACAGCCAATGACATAGCCCATGAAATTCAGAGAGTAACCCCAT5930.16440482956514507No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5850.16218688920001662No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5810.16107791901745241No Hit
CTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA5790.1605234339261703No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5770.1599689488348882No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG5760.15969170628924714No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT5710.15830549356104187No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT5570.15442409792206713No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTCTATTA5290.1466613066441176No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG5260.14582957900719443No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5250.14555233646155338No Hit
TAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT5230.14499785137027127No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTC5170.14333439609642495No Hit
CTCTTGGACTCTCTGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA5100.1413936982769376No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCACCTCGCAGACGAAGAT5100.1413936982769376No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA5070.1405619706400144No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT4990.13834403027488598No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT4960.13751230263796282No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4890.13557160481847544No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4870.13501711972719335No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC4840.1341853920902702No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC4760.13196745172514174No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC4760.13196745172514174No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4690.13002675390565438No Hit
GTATGGATCGGCAGAGGAGCCTGAAAGGTTCCACGCATGCGCTGATGGCC4620.128086056086167No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4620.128086056086167No Hit
GTCATTGGCTGTTATGGGTCATTGCCACAAGAACACATCACACAAAAAAT4580.12697708590360277No Hit
GAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTCTTTAATCCTCAT4570.12669984335796172No Hit
GTACGACATCTCCTAGATACCGCCTCAGCTCTGTATCGGGATGCCTTAGA4570.12669984335796172No Hit
ATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA4560.12642260081232065No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4550.1261453582666796No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG4540.12586811572103856No Hit
CTCCTAGATACCGCCTCAGCTCTGTATCGGGATGCCTTAGAGTCTCCTGA4480.12420466044719222No Hit
GTATCGGGATGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTG4480.12420466044719222No Hit
CCTGTATTCCCATCCCATCATCATGGGCTTTCGGAAAATTCCTATGGGAG4480.12420466044719222No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4450.12337293281026906No Hit
GGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGTCTTTTGGGTTTT4410.12226396262770484No Hit
GACCAGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC4360.12087774989949958No Hit
GTAATGATTAACTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACT4330.12004602226257642No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACCGTGG4310.11949153717129432No Hit
CCATATACTTGGGAACAAGAGCTACAGCATGGGGCAGAATCTTTCCACCA4270.11838256698873009No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4180.1158873840779606No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4150.11505565644103743No Hit
CTCTCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACCACGGGGCGCACCT4140.11477841389539638No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4120.11422392880411428No Hit
GCCAATGACATAGCCCATGAAATTCAGAGAGTAACCCCATCTTTTTGTTT4080.11311495862155005No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT4050.11228323098462689No Hit
CCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGT4010.11117426080206269No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3980.11034253316513952No Hit
GACATAGCCCATGAAATTCAGAGAGTAACCCCATCTTTTTGTTTTGTTAG3960.10978804807385742No Hit
CTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC3950.10951080552821636No Hit
GTACAAAGTAATGATTAACTAGTACAAAGACCTTTAATCTAATCTCCTCC3940.1092335629825753No Hit
ATATTCTATTACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACC3940.1092335629825753No Hit
CTCCGAAAGACACCAAATATTCTATTACTGTTTCTCTTCCAAAAGTGAGA3910.10840183534565213No Hit
GTCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG3900.1081245928000111No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA3890.10784735025437003No Hit
GCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACCACG3880.10757010770872898No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCG3860.10701562261744688No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC3850.10673838007180582No Hit
GCTTTAATGCCCTTGTATGCATGTGTTCAATCTAAGCAGGCTTTCACTTT3840.10646113752616478No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAATTCTTGCCC3830.10618389498052372No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTTTTGTGGTTTCACATTTCTT3820.10590665243488265No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT3810.1056294098892416No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTGTTCAATCTAAGC3800.10535216734360055No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT3780.10479768225231845No Hit
GTGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGCTTGGTCATGG3760.10424319716103633No Hit
CTCTATGGCAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC3740.10368871206975422No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3740.10368871206975422No Hit
ACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTG3740.10368871206975422No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG3720.10313422697847212No Hit
CTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGTTTTTGCCTTCT3710.10285698443283106No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT3690.10230249934154896No Hit
GTATATGGTGACCCACAAAATGAGGCGCTATGTGTTGTTTCTCTCTTATA3680.1020252567959079No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG3650.10119352915898473No Hit
GAGAAACACTCATCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACC3620.10036180152206158No Hit
ACATTGACCCTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGTTT3620.10036180152206158No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA3610.10008455897642052No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)