FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509568
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC45140.8858484049233861No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG43840.8603365988445114No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC40360.7920434564179855No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT34570.6784177970359206No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG31380.6158157498116051No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTTAT30060.5899114544084402No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC26630.52259953529264No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT21100.41407623712635017No Hit
GTACCAAACCTTCGGACGGCAATTGCACCTGTATTCCCATCCCATCATCT21010.41231003516704345No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA20550.40328278070836476No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC20540.4030865360462195No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT18400.36109017834714896No Hit
ATGCGGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG18150.35618406179351925No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG17930.35186667922632503No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG17400.34146571213263No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT17180.33714832956543583No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA17000.3336159256468224No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG16700.3277285857824667No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA16100.31595390605375534No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGGAAAATTCCTATGGGAG15730.3086928535543833No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT15640.30692665159507665No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT15400.30221677970359206No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC15390.3020205350414469No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA14830.29103083396131624No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14820.29083458929917105No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG14630.28710594071841244No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC14390.2823960688269279No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC14300.2806298668676212No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGCTT13790.27062138909821654No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGCAATTGCACC13290.26080915599095705No Hit
GGGTTACTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCAT13160.2582579753830696No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG13090.25688426274805326No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT12940.2539405928158754No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA12820.2515856568701331No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC12720.24962321024868123No Hit
AGTCTGTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT12670.24864198693795528No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA12330.24196966842501885No Hit
ACCCATAACATCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCAT12320.24177342376287364No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC12100.23745604119567945No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT12080.2370635518713891No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT12000.2354935945742276No Hit
ATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATAC11850.23254992464204974No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC11630.22823254207485558No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC11610.22784005275056518No Hit
GGTTTGATACAGCAACAGGAGGGATACATAGAGGTTCCTTGAGCAGGAGT11560.2268588294398392No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA11370.22313018085908065No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGCAATTGC11300.22175646822406428No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG11260.22097148957548354No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA11190.21959777694046723No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT10990.21567288369756343No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA10380.20370195930670687No Hit
ATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATAAA10130.19879584275307713No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA10120.19859959809093194No Hit
CATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAA10060.19742213011806078No Hit
GTAAAAGCCTGCTTAGATTGAATACATGCATACAAGGGCATTAAAGCAGG10010.19644090680733484No Hit
GTTCAGGAACAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCA9640.18917985430796283No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC9550.18741365234865612No Hit
ACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTT9480.1860399397136398No Hit
GATTGAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGT9350.18348875910575232No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC9290.1823112911328812No Hit
GAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC9020.17701268525496106No Hit
AGATCACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTA9010.17681644059281587No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAC9000.1766201959306707No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT8950.17563897261994474No Hit
GACCCATAACATCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCA8930.17524648329565437No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGC8820.17308779201205726No Hit
GGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACA8770.17210656870133131No Hit
CACTTGGCACAGACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGT8640.16955538809344384No Hit
CAATGACATAGCCCATAAAATGTAAAGAGTAACCCCATCTTTTTGTTTTG8560.16798543079628234No Hit
GACATAGCCCATAAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAG8490.16661171816126602No Hit
GCTTAGATTGAATACATGCATACAAGGGCATTAAAGCAGGATAACCACAT8490.16661171816126602No Hit
GCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGC8390.16464927153981412No Hit
GAATACAGGTGCAATTGCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA8330.16347180356694296No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGC8250.16190184626978146No Hit
ATCTTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCT8240.16170560160763625No Hit
GCCCATGACCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGC8230.1615093569454911No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTG8050.15797695302687767No Hit
GGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA8020.1573882190404421No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA7960.15621075106757096No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTATCAAGGTATGTTGC7890.15483703843255464No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC7760.15228585782466716No Hit
ACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC7690.15091214518965085No Hit
GGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCC7650.15012716654107008No Hit
CTCTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACA7510.14737974127103742No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC7500.14718349660889224No Hit
ATAACATCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCATCTTT7440.1460060286360211No Hit
GTCTCTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAA7430.14580978397387592No Hit
CAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGCTTAGATTGAA7280.14286611404169808No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTTACTTTCTCGCCAACTTACA7230.14188489073097213No Hit
CTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATG7230.14188489073097213No Hit
GGTTCTGCATGGTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGAC7220.14168864606882692No Hit
ACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCGAGGATT7210.14149240140668173No Hit
TCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCATCTTTTTGTTT7200.14129615674453655No Hit
ACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGG7130.1399224441095202No Hit
GCCCATAAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTA7130.1399224441095202No Hit
ACAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTT7020.13776375282592315No Hit
CCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT6950.1363900401909068No Hit
GTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAAT6950.1363900401909068No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG6850.1344275935694549No Hit
GAACTACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC6840.13423134890730973No Hit
GCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAA6810.13364261492087415No Hit
GCATGGTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGG6790.13325012559658378No Hit
ACCCAAAAGACCCACAATACGTTGACAGACTTTCCAATCAATAGGCCTGT6760.13266139161014823No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTTACTTT6740.13226890228585783No Hit
GCCCAAGATGATGGGATGGGAATACAGGTGCAATTGCCGTCCGAAGGTTT6670.13089518965084151No Hit
CTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA6660.1306989449886963No Hit
CTCCCATAGGAATTTTCCGAAAGCCCAAGATGATGGGATGGGAATACAGG6640.13030645566440593No Hit
GCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCTGGCC6540.128344009042954No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCT6530.12814776438080885No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG6400.12559658377292138No Hit
CCCATAGGAATTTTCCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6370.1250078497864858No Hit
TCGTAGGGCTTTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGG6370.1250078497864858No Hit
TTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGTT6350.12461536046219543No Hit
TGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCCCTCCTG6320.12402662647575985No Hit
CCCATAACATCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCATC6320.12402662647575985No Hit
GCACAGACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTT6310.12383038181361468No Hit
TCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTGCTGGTGGTTG6310.12383038181361468No Hit
CCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACTCAAGATG6290.12343789248932428No Hit
CATCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCATCTTTTTGT6280.1232416478271791No Hit
ATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCCCTCCT6250.12265291384074353No Hit
GTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACAT6240.12245666917859833No Hit
GTCCCCAACCTCCAATCACTCACCAACCTCCTGTCCTCCAACTTGTCCTG6230.12226042451645314No Hit
GTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6220.12206417985430797No Hit
CTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCA6200.12167169053001758No Hit
CATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCA6200.12167169053001758No Hit
AGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAATTT6170.12108295654358202No Hit
ACTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA6160.12088671188143682No Hit
CCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAG6140.12049422255714644No Hit
ACCTCCAATCACTCACCAACCTCCTGTCCTCCAACTTGTCCTGGTTATCG6120.12010173323285607No Hit
CCCCAACCTCCAATCACTCACCAACCTCCTGTCCTCCAACTTGTCCTGGT6080.11931675458427532No Hit
ATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCT6070.11912050992213012No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA6040.11853177593569456No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACCGCATCTTGAGTCCCTTTTTACC6030.11833553127354936No Hit
CCTGTTAACAGGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCT6030.11833553127354936No Hit
CAATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCC6000.1177467972871138No Hit
GTATCAAACCTTCGGACGGCAATTGCACCTGTATTCCCATCCCATCATCT5990.11755055262496861No Hit
ATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGCAA5990.11755055262496861No Hit
CTCACCAACCTCCTGTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTC5970.11715806330067821No Hit
CCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGG5950.11676557397638784No Hit
TCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTAT5940.11656932931424266No Hit
TTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTTATGG5930.11637308465209746No Hit
TCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTA5930.11637308465209746No Hit
CATGTATTCAATCTAAGCAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5880.11539186134137151No Hit
GGATGAAGAGGAAGATGATAAAACGCCGCAGACACATCCAGCGATAACCA5840.11460688269279075No Hit
GGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCC5830.11441063803064556No Hit
GGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGAT5830.11441063803064556No Hit
ATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG5830.11441063803064556No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5800.11382190404421No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGCT5800.11382190404421No Hit
CAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTT5770.11323317005777443No Hit
GCGCTGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGT5740.11264443607133885No Hit
GGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGA5720.11225194674704848No Hit
GTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGCTTA5700.1118594574227581No Hit
GTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCC5670.11127072343632254No Hit
CAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5660.11107447877417734No Hit
GTTTGGTACAGCAACAGGAGGGATACATAGAGGTTCCTTGAGCAGGAGTC5580.10950452147701582No Hit
CCTCCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCAT5570.10930827681487063No Hit
TTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACA5470.10734583019341873No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC5460.10714958553127356No Hit
CTGATGGCCCATGACCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACA5420.1063646068826928No Hit
GGGATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTC5400.10597211755840241No Hit
ATTGGGGACCCTGCGCTGAACATGGAGAACATCACATCAGGATTCCTAGG5380.10557962823411202No Hit
CAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTG5340.10479464958553127No Hit
GTTCTGCATGGTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACA5290.10381342627480532No Hit
GTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAAAC5280.10361718161266015No Hit
GACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG5240.10283220296407938No Hit
GTATTCCCATCCCATCATCTTGGGCTTTCGGAAAATTCCTATGGGAGTGG5190.10185097965335344No Hit
ATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGCAATTGCACCTG5180.10165473499120824No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG5120.10047726701833709No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)