FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509568
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG48230.9464880055262496No Hit
GTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC42260.8293299422255715No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC40270.7902772544586788No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT33120.6499623210248681No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTTAT31310.6144420371765889No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG29810.5850053378548103No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC26000.5102361215774931No Hit
GTACCAAACCTTCGGACGGCAATTGCACCTGTATTCCCATCCCATCATCT21660.4250659382064808No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT21450.42094480030143183No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT19470.38208835719668427No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA18750.3679587415222306No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC18630.3656038055764883No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG17910.35147418990203466No Hit
ATGCGGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG17540.34421313740266263No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA17500.3434281587540819No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT17310.3396995101733233No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT16770.32910229841748306No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC16560.32498116051243403No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG15480.3037867370007536No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA15130.2969181738256719No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC14860.29161956794775185No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14800.2904420999748807No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT14750.28946087666415476No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG14670.28789091936699324No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGGAAAATTCCTATGGGAG14650.28749843004270287No Hit
ATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATAC14460.28376978146194426No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA14310.2808261115297664No Hit
GGGTTACTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCAT14280.28023737754333083No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT13970.2741537930168299No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGCAATTGCACC13570.2663040065310224No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT13340.26179037930168303No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC13320.2613978899773926No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG13160.2582579753830696No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA13110.25727675207234363No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG12950.2541368374780206No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC12940.2539405928158754No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGCTT12550.24628705099221299No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT12510.24550207234363225No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA12190.23922224315498616No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA12170.23882975383069582No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT12070.23686730720924393No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGCAATTGC11980.23510110524993721No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC11870.2329424139663401No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA11560.2268588294398392No Hit
ACCCATAACATCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCAT11490.2254851168048229No Hit
GGTTTGATACAGCAACAGGAGGGATACATAGAGGTTCCTTGAGCAGGAGT11280.22136397889977394No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC11270.22116773423762875No Hit
AGTCTGTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT11160.21900904295403165No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC11100.21783157498116051No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT10770.21135550113036924No Hit
ATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATAAA10650.20900056518462698No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA10650.20900056518462698No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG10630.2086080758603366No Hit
GTTCAGGAACAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCA10560.2072343632253203No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGC10290.20193575734740016No Hit
AGATCACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTA10000.19624466214518965No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC9770.19173103491585028No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA9710.19055356694297915No Hit
GAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC9550.18741365234865612No Hit
GTAAAAGCCTGCTTAGATTGAATACATGCATACAAGGGCATTAAAGCAGG9500.18643242903793017No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAC9450.18545120572720422No Hit
CATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAA9400.18446998241647827No Hit
GACATAGCCCATAAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAG9250.18152631248430043No Hit
GGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACA9080.17819015322783222No Hit
GATTGAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGT8870.1740690153227832No Hit
ACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGG8760.17191032403918613No Hit
ATCTTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCT8700.170732856066315No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTG8610.16896665410700829No Hit
GACCCATAACATCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCA8590.16857416478271792No Hit
GCTTAGATTGAATACATGCATACAAGGGCATTAAAGCAGGATAACCACAT8470.16621922883697562No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC8410.1650417608641045No Hit
GCCCATGACCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGC8390.16464927153981412No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC8360.16406053755337854No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGC8360.16406053755337854No Hit
GTCTCTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAA8340.16366804822908818No Hit
GAACTACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC8340.16366804822908818No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTATCAAGGTATGTTGC8320.1632755589047978No Hit
ACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTT8270.16229433559407186No Hit
GAATACAGGTGCAATTGCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA8200.16092062295905551No Hit
GGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA8130.1595469103240392No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA8110.1591544209997488No Hit
CTCTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACA8100.15895817633760362No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTTACTTTCTCGCCAACTTACA8020.1573882190404421No Hit
ACAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTT7970.15640699572971614No Hit
CAATGACATAGCCCATAAAATGTAAAGAGTAACCCCATCTTTTTGTTTTG7900.15503328309469983No Hit
ACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCGAGGATT7830.1536595704596835No Hit
CACTTGGCACAGACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGT7730.1516971238382316No Hit
ACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC7710.15130463451394122No Hit
CTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATG7710.15130463451394122No Hit
GTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAAT7700.15110838985179603No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC7660.15032341120321527No Hit
GCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGC7590.14894969856819895No Hit
GTCCCCAACCTCCAATCACTCACCAACCTCCTGTCCTCCAACTTGTCCTG7420.14561353931173074No Hit
TCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCATCTTTTTGTTT7420.14561353931173074No Hit
TCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTA7340.1440435820145692No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTTACTTT7290.14306235870384326No Hit
GGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCC7270.14266986937955287No Hit
ATAACATCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCATCTTT7260.14247362471740768No Hit
ATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCT7180.14090366742024615No Hit
TTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTTATGG7160.1405111780959558No Hit
ATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCCCTCCT7050.1383524868123587No Hit
GTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA7030.1379599974880683No Hit
GCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAA6990.13717501883948754No Hit
CAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGCTTAGATTGAA6900.13540881688018086No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCT6890.13521257221803568No Hit
GTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACAT6880.1350163275558905No Hit
GCCCAAGATGATGGGATGGGAATACAGGTGCAATTGCCGTCCGAAGGTTT6840.13423134890730973No Hit
CTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA6750.13246514694800302No Hit
GCCCATAAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTA6740.13226890228585783No Hit
CTCCCATAGGAATTTTCCGAAAGCCCAAGATGATGGGATGGGAATACAGG6700.13148392363727707No Hit
GGTTCTGCATGGTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGAC6680.13109143431298667No Hit
CATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCA6670.13089518965084151No Hit
CCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAG6600.12952147701582517No Hit
CCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACTCAAGATG6600.12952147701582517No Hit
CCCCAACCTCCAATCACTCACCAACCTCCTGTCCTCCAACTTGTCCTGGT6580.1291289876915348No Hit
ACTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA6550.12854025370509922No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG6520.12795151971866364No Hit
CCTCCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCAT6510.12775527505651846No Hit
TTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGTT6510.12775527505651846No Hit
GCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCTGGCC6500.12755903039437327No Hit
CATCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCATCTTTTTGT6480.1271665410700829No Hit
CCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGG6470.1269702964079377No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG6450.12657780708364733No Hit
CTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCA6410.12579282843506656No Hit
TGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCCCTCCTG6400.12559658377292138No Hit
ACCTCCAATCACTCACCAACCTCCTGTCCTCCAACTTGTCCTGGTTATCG6390.1254003391107762No Hit
CAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTT6360.12481160512434061No Hit
ACCCAAAAGACCCACAATACGTTGACAGACTTTCCAATCAATAGGCCTGT6330.12422287113790505No Hit
CTCACCAACCTCCTGTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTC6270.12304540316503393No Hit
GTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCC6240.12245666917859833No Hit
CCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT6140.12049422255714644No Hit
GTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTC6130.12029797789500127No Hit
TCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTAT6070.11912050992213012No Hit
AGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAATTT6050.11872802059783973No Hit
GCACAGACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTT6040.11853177593569456No Hit
ATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG6040.11853177593569456No Hit
TCGTAGGGCTTTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGG5990.11755055262496861No Hit
GCGCTGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGT5980.11735430796282341No Hit
GTATTCCCATCCCATCATCTTGGGCTTTCGGAAAATTCCTATGGGAGTGG5970.11715806330067821No Hit
CCTGTTAACAGGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCT5920.11617683998995226No Hit
GTTTTATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTG5910.11598059532780709No Hit
CCCATAACATCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCATC5900.1157843506656619No Hit
CAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5880.11539186134137151No Hit
GCATGGTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGG5860.11499937201708114No Hit
GGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGA5850.11480312735493595No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5840.11460688269279075No Hit
GTATCAAACCTTCGGACGGCAATTGCACCTGTATTCCCATCCCATCATCT5800.11382190404421No Hit
TCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTGCTGGTGGTTG5770.11323317005777443No Hit
CAATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCC5750.11284068073348405No Hit
GGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCC5720.11225194674704848No Hit
CCCATAGGAATTTTCCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5710.11205570208490329No Hit
CCTCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTC5680.11146696809846773No Hit
TTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACA5660.11107447877417734No Hit
GTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAAAC5650.11087823411203214No Hit
GGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGAT5560.10911203215272544No Hit
ATTGGGGACCCTGCGCTGAACATGGAGAACATCACATCAGGATTCCTAGG5560.10911203215272544No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA5540.10871954282843507No Hit
CTGATGGCCCATGACCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACA5540.10871954282843507No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGCT5520.10832705350414468No Hit
ATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGCAATTGCACCTG5520.10832705350414468No Hit
TTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC5490.10773831951770912No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5450.10695334086912836No Hit
GTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGCTTA5430.10656085154483798No Hit
GTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATC5360.10518713890982165No Hit
GTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTGTGTCTTGGCC5340.10479464958553127No Hit
CCTCCAATCACTCACCAACCTCCTGTCCTCCAACTTGTCCTGGTTATCGC5270.10342093695051495No Hit
ATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGCAA5270.10342093695051495No Hit
CATGTATTCAATCTAAGCAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5270.10342093695051495No Hit
GTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGCAG5250.10302844762622455No Hit
CTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA5200.1020472243154986No Hit
GTTCTGCATGGTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACA5150.10106600100477268No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)