FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578711
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN87081.5047234284470141No Hit
GCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA36420.6293296654115785No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT25590.4421896248732096No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT22390.38689432203638774No Hit
CAATTCGTTGACACACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTT21490.37134251811353164No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTCTGAATTTCAT19550.3378197407687084No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT17280.2985946353188379No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT14340.2477920758375078No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAT14310.24727368237341263No Hit
CTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTT13290.22964830459417568No Hit
CTCTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGT12980.22429157213185855No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT12930.2234275830250332No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAATCTAGCAGG12750.22031722224046202No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGT11770.2033830357466853No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC10980.18973200785884492No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTG10830.18714004053836888No Hit
TAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT10630.1836840841110675No Hit
TTCTATTACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA10510.1816105102546867No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGACACACT10050.17366181047189358No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTT10020.17314341700779837No Hit
CACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGT9970.17227942790097303No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT9810.16951466275913193No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC9740.16830507800957648No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT9410.1626027499045292No Hit
ATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA9390.16225715426179907No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9320.16104756951224358No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGG9070.1567276239781169No Hit
GTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGATTTTATCC9060.15655482615675181No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8800.15206208280126005No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA8760.15137089151579977No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA8730.15085249805170456No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC8620.1489517220166888No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT8610.14877892419532374No Hit
CTATGAATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC8490.1467053503389429No Hit
CTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC8350.14428618083983197No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCT8330.14394058519710184No Hit
GATTAACTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA8310.1435949895543717No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC8270.14290379826891142No Hit
GTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGG8250.1425582026261813No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT8110.14013903312707032No Hit
CAGTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGATTTTAT7900.1365102788784039No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT7810.13495509848611828No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT7810.13495509848611828No Hit
GTTTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTA7710.1332271202724676No Hit
CTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACCGTGGA7410.12804318563151557No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGTGTGATGTGTT7320.12648800523922993No Hit
GGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG7310.12631520741786487No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGAACACATCACACAAAAA7280.12579681395376965No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG7240.12510562266830938No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG7060.12199526188373817No Hit
GTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA7050.12182246406237311No Hit
GAAAAGAAGATTGCAATTGATTATGCCTGCTAGATTTTATCCAAATGTTA7030.12147686841964297No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6940.11992168802735735No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC6830.1180209119923416No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG6790.11732972070688133No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA6700.1157745403145957No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA6680.11542894467186557No Hit
CTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGATT6660.11508334902913543No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6600.11404656210094502No Hit
GGCATAATCAATTGCAATCTTCTTTTCTCATTAACTGTGAGTGGGCCTAC6480.1119729882445642No Hit
GTTATGGGTCATTGCCACAAGAACACATCACACAAAAAATCAAAGAATGT6470.11180019042319914No Hit
CCTACAAACTGTTCACATTTTTTGATAATGTCTTGGTGTAAATGTATATT6450.111454594780469No Hit
CTCCGAAAGACACCAAATATTCTATTACTGTTTCTCTTCCAAAAGTGAGA6430.11110899913773888No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC6430.11110899913773888No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA6420.11093620131637381No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6400.11059060567364368No Hit
ACCCATAACATCCAATGACATAGCCCATGAAATTCAGAGAGTAACCCCAT6290.10868982963862792No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT6220.10748024488907244No Hit
CTATTACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAG6210.10730744706770737No Hit
GTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACA6170.1066162557822471No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC6140.1060978623181519No Hit
CTCTTGGACTCTCTGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA6120.10575226667542176No Hit
CATTTGGATAAAATCTAGCAGGCATAATCAATTGCAATCTTCTTTTCTCA6070.10488827756859641No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG6010.103851490640406No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5990.10350589499767586No Hit
GACATAGCCCATGAAATTCAGAGAGTAACCCCATCTTTTTGTTTTGTTAG5980.1033330971763108No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACCGTGG5970.10316029935494572No Hit
GGCAAATATTTGGTAACATTTGGATAAAATCTAGCAGGCATAATCAATTG5970.10316029935494572No Hit
CTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCT5910.10212351242675531No Hit
GATTATGCCTGCTAGATTTTATCCAAATGTTACCAAATATTTGCCATTGG5900.10195071460539025No Hit
GTATATGGTGACCCACAAAATGAGGCGCTATGTGTTGTTTCTCTCTTATA5850.1010867254985649No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5850.1010867254985649No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTCTGA5820.1005683320344697No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC5800.10022273639173956No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)