FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523647
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA24530.46844534581502423No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC19590.37410698428521505No Hit
CCTACTGCATATAGACCCCCAAATGCCCCTATCTTATCAACACTTCCGGA17750.33896880914050875No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC14080.26888342719427405No Hit
ATGTAAGACCTTGGGCAGGTTCCGGTGGGCGTTCACGGTGGTCTCCATGC14020.2677376171352075No Hit
CCCTTATCTCTACCTCTAAGGGACACTCATCCTCAGGCCGTGCAGTGGAA13260.2532240230536984No Hit
GTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCGTTGACATACT13210.2522691813378096No Hit
GTTGTGAGTATGCCCTGAGCCAGAGGGCTCCACCCCAACAAGCCCCCATG13140.25093240293556535No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG12730.24310270086527758No Hit
ATACCATATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA12420.23718268222676728No Hit
TCCTCAGGCCGTGCAGTGGAACTCCACCACTTTCCATCAAACGCTTCAAG12290.23470009376545647No Hit
CATTTAAACCCTCACAAAACCAAACGATGGGGATATTCCCTTAATTTCAT11600.22152327808619166No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG11580.22114134139983613No Hit
ATATAAAAGAGAGACGACACGTAGCGCCTCATTTTGCGGGTCACCATATT11550.22056843637030288No Hit
ATATGGTATTGGGGGCCAAGACTGTACAACATCTTGAGTCCCTTTATGCC11360.21694003784992563No Hit
GTCTTCGAGAAGGTTGACGATATGGCAGAGGCAGTATTCTGAGCAGGGCT11320.2161761644772146No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA11210.21407551270225936No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT11130.21254776595683733No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG10940.20891936743646006No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG10770.20567290560243828No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT10690.20414515885701628No Hit
CCAAAAGACACCAAATATTCAAGAACCGTTTCTCTTCCAAAAGTAAGACA9820.1875309130005519No Hit
CTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT9680.18485735619606336No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC9310.17779152749848656No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTCAATC9280.17721862246895334No Hit
ATATGTTCCTGTGGCAAGGAGCCCCAACTCCCAATTACATATCCCATGAA9200.17569087572353131No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC9160.17492700235082032No Hit
ATTCCGTCGTGCTCTCCCGCAAGTATACATCGTTTCCATGGCTGCTAGGC9140.17454506566446482No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT9030.17244441388950954No Hit
GAATGCAGGGTCCAACTGATGATCGGGGAAGAATCCCAGTGGATTGGGGA9000.17187150885997626No Hit
CTCCTACTGCATATAGACCCCCAAATGCCCCTATCTTATCAACACTTCCG8780.16767020531006574No Hit
GGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAAACAGAAGATTG8760.16728826862371024No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGAAT8670.1655695535351105No Hit
GTGGCAAGGAGCCCCAACTCCCAATTACATATCCCATGAAATTAAGGGAA8590.16404180678968847No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8330.15907662986706694No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA8310.15869469318071144No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCGGTG8220.15697597809211167No Hit
CGACAGAATTGTCAGTCCCGATAAGTTTTGCCCCAGACCGGCTGCGAGCA8140.15544823134668967No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC8140.15544823134668967No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT8100.15468435797397867No Hit
GTCTTGGCCCCCAATACCATATCATCCATATAACTGAAAGCCAGACAGTG7890.15067402276724587No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC7810.14914627602182387No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGACGACACGTAGCGCC7800.14895530767864612No Hit
GTATGGATCGGCAGCGGAGACATAAAGGTTCCGCGCATGCGCTGATGGCC7760.14819143430593512No Hit
CCCACTCAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTT7740.1478094976195796No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7630.14570884584462435No Hit
ATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA7430.14188947898106932No Hit
GTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTTTA7400.14131657395153605No Hit
GTATAAAGAATTTGGAGCTTCTGGAGAATTACTCTCTTTTTTGCCTTCTG7270.1388339854902253No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA7270.1388339854902253No Hit
GTATTGGGGGCCAAGACTGTACAACATCTTGAGTCCCTTTATGCCGCTGT7100.1355875236562035No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACATACTTTGAGTTTTTGT7090.13539655531302577No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATATGGTATTGGGGGC7020.1340597769107815No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTACGG6970.13310493519489275No Hit
GTTTACAGGAAGTTTCCTAAAACATACTTTGAGTTTTTGTACAATATGTT6960.132913966851715No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA6940.1325320301653595No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATTCCTATGGGAG6930.13234106182218175No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC6920.13215009347900397No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC6900.13176815679264847No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTAGAGATAAGGGAGTAGGCTGCCT6890.13157718844947075No Hit
GCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCGACGACTGGGGA6860.13100428341993747No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCG6850.13081331507675972No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA6830.13043137839040422No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA6770.1292855683313377No Hit
GCTTACACCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA6770.1292855683313377No Hit
CTGTTAGGGGTCCCACAAATTGCTTACACCTATCAACAATGTCCTCCTGC6770.1292855683313377No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6760.12909459998815995No Hit
GTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCCGCCTGTAACACG6740.12871266330180448No Hit
CCTTACCACAGGAACATATTGTACAAAAACTCAAAGTATGTTTTAGGAAA6700.12794878992909348No Hit
AGATAAGGGAGTAGGCTGCCTTCCTGACTGCCGATTGGTGGAGGCAGGAG6660.12718491655638245No Hit
GAATAGAGGGTCCAACTGATGATCGGGGAAGAATCCCAGTGGATTGGGGA6650.1269939482132047No Hit
CCCAATTACATATCCCATGAAATTAAGGGAATATCCCCATCGTTTGGTTT6570.12546620146778267No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6550.1250842647814272No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT6530.12470232809507167No Hit
CCTTGTTGGCATCCGGCCAGTTGTCCTTGTGTGGATTGAGGTCCCAATCT6530.12470232809507167No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAAT6480.12374748637918292No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA6430.12279264466329416No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6420.12260167632011641No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAGGTTCCGGTGGGCGTTC6400.12221973963376091No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6360.12145586626104991No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG6310.12050102454516115No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGAGAGCACGACGGAAT6260.1195461828292724No Hit
TTCAAAGCCAACTCAGAAAATCCAGATTGGGACCTCAATCCACACAAGGA6260.1195461828292724No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC6240.11916424614291689No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6200.11840037277020589No Hit
CTCGACGACTGGGGACCCTGTACCGAACATGGAGAACATCGCATCAGGAC6190.11820940442702813No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG6110.11668165768160613No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT6070.11591778430889513No Hit
ACCAAATACTTGCCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCA6070.11591778430889513No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG6010.11477197424982861No Hit
ATGCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA5980.11419906922029534No Hit
GGAACATATTGTACAAAAACTCAAAGTATGTTTTAGGAAACTTCCTGTAA5950.1136261641907621No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT5950.1136261641907621No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT5880.11228938578851783No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA5860.11190744910216234No Hit
CTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGG5850.11171648075898458No Hit
GACATAAAGGTTCCGCGCATGCGCTGATGGCCTATGGCCAAGCCCCAACC5820.11114357572945134No Hit
GTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT5810.11095260738627358No Hit
ATGTTGTACAGTCTTGGCCCCCAATACCATATCATCCATATAACTGAAAG5760.10999776567038483No Hit
CCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5740.10961582898402931No Hit
GTTGATAGGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAAACAG5700.10885195561131832No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG5630.10751517720907407No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACTGCATATAGACCCCCAAATGCC5600.10694227217954079No Hit
ATTTACATACTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGACGACA5600.10694227217954079No Hit
GGATTAAAGTTAGGTACAGTAGAAGAATAAAGCCCCGTAAAGTTTCCCAC5600.10694227217954079No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAAGGGGCAGCG5590.10675130383636304No Hit
TCTCGACGACTGGGGACCCTGTACCGAACATGGAGAACATCGCATCAGGA5590.10675130383636304No Hit
CCTTCCAGGCATCCGGCCAGTTGTCCTTGTGTGGATTGAGGTCCCAATCT5570.10636936715000755No Hit
GGCCAAGACTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATT5530.10560549377729654No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAG5520.10541452543411879No Hit
ATGTAATTGGGAGTTGGGGCTCCTTGCCACAGGAACATATTGTACAAAAA5470.10445968371823003No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAATACTAAC5460.10426871537505228No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA5460.10426871537505228No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC5440.10388677868869677No Hit
ATCTTATATAAAAGAGAGACGACACGTAGCGCCTCATTTTGCGGGTCACC5370.10255000028645252No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCC5370.10255000028645252No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA5360.10235903194327477No Hit
ATTTATGCCTACAGCCTCCTAATACAAAGATCATTAACCTAAACTCCTCC5360.10235903194327477No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAA5340.10197709525691925No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC5310.101404190227386No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGTGA5300.10121322188420825No Hit
GCATATAGACCCCCAAATGCCCCTATCTTATCAACACTTCCGGAAACTAC5300.10121322188420825No Hit
CTCCAAAAGACACCAAATATTCAAGAACCGTTTCTCTTCCAAAAGTAAGA5290.10102225354103052No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGGAGA5280.10083128519785275No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)