FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523647
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA19140.3655134088422162No Hit
CCTACTGCATATAGACCCCCAAATGCCCCTATCTTATCAACACTTCCGGA18840.3597843585468837No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC18490.3531004665356624No Hit
CCCTTATCTCTACCTCTAAGGGACACTCATCCTCAGGCCGTGCAGTGGAA14080.26888342719427405No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC13180.2516962763082764No Hit
GTTGTGAGTATGCCCTGAGCCAGAGGGCTCCACCCCAACAAGCCCCCATG13000.24825884613107688No Hit
TCCTCAGGCCGTGCAGTGGAACTCCACCACTTTCCATCAAACGCTTCAAG12700.24252979583574433No Hit
GTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCGTTGACATACT12030.22973491684283495No Hit
CATTTAAACCCTCACAAAACCAAACGATGGGGATATTCCCTTAATTTCAT11730.2240058665475024No Hit
ATACCATATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA11570.2209503730566584No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT11440.21846778459534763No Hit
ATATGGTATTGGGGGCCAAGACTGTACAACATCTTGAGTCCCTTTATGCC11410.21789487956581435No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG11080.21159292424094855No Hit
ATTCCGTCGTGCTCTCCCGCAAGTATACATCGTTTCCATGGCTGCTAGGC10970.2094922724659933No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG10930.20872839909328228No Hit
GTCTTCGAGAAGGTTGACGATATGGCAGAGGCAGTATTCTGAGCAGGGCT10770.20567290560243828No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA10420.19898901359121698No Hit
ATATAAAAGAGAGACGACACGTAGCGCCTCATTTTGCGGGTCACCATATT10380.19822514021850599No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT10340.19746126684579499No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG10050.1919231848936402No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTCAATC9810.18733994465737416No Hit
CTCCTACTGCATATAGACCCCCAAATGCCCCTATCTTATCAACACTTCCG9810.18733994465737416No Hit
CTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT9730.18581219791195214No Hit
ATGTAAGACCTTGGGCAGGTTCCGGTGGGCGTTCACGGTGGTCTCCATGC9430.1800831476166196No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC9250.17664571743942006No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT9020.1722534455463318No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC8990.17168054051679854No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG8960.17110763548726526No Hit
ATATGTTCCTGTGGCAAGGAGCCCCAACTCCCAATTACATATCCCATGAA8960.17110763548726526No Hit
GAATGCAGGGTCCAACTGATGATCGGGGAAGAATCCCAGTGGATTGGGGA8550.16327793341697747No Hit
CCAAAAGACACCAAATATTCAAGAACCGTTTCTCTTCCAAAAGTAAGACA8530.16289599673062197No Hit
GCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCGACGACTGGGGA8450.16136824998519997No Hit
GGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAAACAGAAGATTG8190.15640307306257842No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC8140.15544823134668967No Hit
GTCTTGGCCCCCAATACCATATCATCCATATAACTGAAAGCCAGACAGTG8140.15544823134668967No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA7890.15067402276724587No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC7890.15067402276724587No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATATGGTATTGGGGGC7770.14838240264911287No Hit
GTGGCAAGGAGCCCCAACTCCCAATTACATATCCCATGAAATTAAGGGAA7750.14800046596275734No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCGGTG7710.14723659259004634No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7710.14723659259004634No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGACGACACGTAGCGCC7700.1470456242468686No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGAAT7580.1447540041287356No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC7500.14322625738331357No Hit
AGATAAGGGAGTAGGCTGCCTTCCTGACTGCCGATTGGTGGAGGCAGGAG7470.14265335235378032No Hit
GTATAAAGAATTTGGAGCTTCTGGAGAATTACTCTCTTTTTTGCCTTCTG7420.14169851063789157No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTAGAGATAAGGGAGTAGGCTGCCT7240.13826108046069205No Hit
GTATTGGGGGCCAAGACTGTACAACATCTTGAGTCCCTTTATGCCGCTGT7170.13692430205844777No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT7160.13673333371527002No Hit
ATGTTGTACAGTCTTGGCCCCCAATACCATATCATCCATATAACTGAAAG7110.13577849199938125No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7060.1348236502834925No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAG7060.1348236502834925No Hit
CCTTACCACAGGAACATATTGTACAAAAACTCAAAGTATGTTTTAGGAAA6960.132913966851715No Hit
GCTTACACCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA6910.13195912513582622No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA6850.13081331507675972No Hit
ATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA6850.13081331507675972No Hit
GTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTTTA6780.12947653667451547No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6730.1285216949586267No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC6720.12833072661544895No Hit
GGAACATATTGTACAAAAACTCAAAGTATGTTTTAGGAAACTTCCTGTAA6690.1277578215859157No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACATACTTTGAGTTTTTGT6690.1277578215859157No Hit
GTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCCGCCTGTAACACG6680.12756685324273795No Hit
GTTTACAGGAAGTTTCCTAAAACATACTTTGAGTTTTTGTACAATATGTT6650.1269939482132047No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATTCCTATGGGAG6600.12603910649731595No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC6590.1258481381541382No Hit
GAATAGAGGGTCCAACTGATGATCGGGGAAGAATCCCAGTGGATTGGGGA6570.12546620146778267No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC6540.12489329643824944No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAAT6520.12451135975189392No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA6490.12393845472236067No Hit
CTGTTAGGGGTCCCACAAATTGCTTACACCTATCAACAATGTCCTCCTGC6430.12279264466329416No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA6380.1218378029474054No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG6370.12164683460422765No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG6310.12050102454516115No Hit
GTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT6310.12050102454516115No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA6260.1195461828292724No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT6250.11935521448609464No Hit
TCTCGACGACTGGGGACCCTGTACCGAACATGGAGAACATCGCATCAGGA6240.11916424614291689No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA6230.11897327779973914No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6220.11878230945656137No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA6220.11878230945656137No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6090.11629972099525061No Hit
CCCAATTACATATCCCATGAAATTAAGGGAATATCCCCATCGTTTGGTTT6010.11477197424982861No Hit
GGATTAAAGTTAGGTACAGTAGAAGAATAAAGCCCCGTAAAGTTTCCCAC6010.11477197424982861No Hit
CCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5990.1143900375634731No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA5990.1143900375634731No Hit
ACCAAATACTTGCCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCA5970.11400810087711762No Hit
ATTTACATACTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGACGACA5940.11343519584758434No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCCCGGGGCCG5910.1128622908180511No Hit
CCTTGTTGGCATCCGGCCAGTTGTCCTTGTGTGGATTGAGGTCCCAATCT5880.11228938578851783No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG5830.11133454407262908No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT5770.11018873401356258No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACTGCATATAGACCCCCAAATGCC5750.10980679732720706No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGCGGAACCTTTATGTC5720.10923389229767382No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC5710.10904292395449607No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT5690.10866098726814055No Hit
ATGTAATTGGGAGTTGGGGCTCCTTGCCACAGGAACATATTGTACAAAAA5680.10847001892496282No Hit
GTATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC5680.10847001892496282No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG5670.10827905058178507No Hit
GTTGATAGGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAAACAG5640.1077061455522518No Hit
TTCTTCTACTGTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTC5630.10751517720907407No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCG5630.10751517720907407No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAATACTAAC5600.10694227217954079No Hit
CTCGACGACTGGGGACCCTGTACCGAACATGGAGAACATCGCATCAGGAC5560.10617839880682979No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA5560.10617839880682979No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGTGA5530.10560549377729654No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT5510.10522355709094103No Hit
CTACGTCAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGG5510.10522355709094103No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGGAGA5500.10503258874776328No Hit
GGCCAAGACTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATT5470.10445968371823003No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG5460.10426871537505228No Hit
CCTTCCAGGCATCCGGCCAGTTGTCCTTGTGTGGATTGAGGTCCCAATCT5460.10426871537505228No Hit
TTCAAAGCCAACTCAGAAAATCCAGATTGGGACCTCAATCCACACAAGGA5460.10426871537505228No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5440.10388677868869677No Hit
CTCCAAAAGACACCAAATATTCAAGAACCGTTTCTCTTCCAAAAGTAAGA5440.10388677868869677No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCC5430.10369581034551903No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5410.10331387365916352No Hit
AGTATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5400.10312290531598577No Hit
ATGCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA5400.10312290531598577No Hit
GACATAAAGGTTCCGCGCATGCGCTGATGGCCTATGGCCAAGCCCCAACC5380.10274096862963028No Hit
CTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGG5330.10178612691374152No Hit
CCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCATGTAGTTAATCA5320.10159515857056375No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)