FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470135
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA33670.7161772682314654No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA25470.5417592819083881No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC24350.5179363374349921No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTTGA19520.4151998893934721No Hit
CTTTCGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTG18900.40201218798855654No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC18490.3932912886724026No Hit
TATCCAAAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA16880.35904580599189595No Hit
ATTTACATACTTTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACA14430.3069331149563423No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC14370.30565688578812467No Hit
GCCTAGTCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC14270.3035298371744286No Hit
CCTCTCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13470.2865134482648601No Hit
GTATGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG13160.2799195975624023No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGT12760.271411403107618No Hit
TATCAAAAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA12580.2675827156029651No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA12250.2605634551777681No Hit
CTTTTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTG12140.2582237017027024No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGC11410.24269624682272112No Hit
AGCGAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCTAGAA11060.2352515766747849No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA9670.20568560094440957No Hit
ACCCATCTCGGGGCCGTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCG9370.19930445510332137No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCGTAT9180.19526306273729885No Hit
GTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC9030.19207248981675476No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG8920.18973273634168908No Hit
GCACTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGGAATTCCA8710.18526593425292737No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT8570.18228806619375285No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA8560.18207536133238325No Hit
ATTGTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA8540.18164995160964403No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8450.17973560785731757No Hit
GACTTTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTGC8360.17782126410499113No Hit
GTTCCCAATCCTCTGGGATTCTTTCCCGGTCACCAGTTGGACCCAGCGTT8330.1771831495208823No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG8240.17526880576855586No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA8170.17377987173896858No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG8120.17271634743212055No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT8090.17207823284801174No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG8080.17186552798664215No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA8070.17165282312527252No Hit
CTTATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCA7960.16931306965020687No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT7940.16888765992746765No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCAACCCAAAAGACTGCCGTG7860.1671860210365108No Hit
GATTTGGAACAGAAAGGTTCGTCCCCATGCCTTGTCGAGGTTTGGAAGAC7850.1669733161751412No Hit
GTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT7830.16654790645240197No Hit
CTATTTACATACTTTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTA7790.16569708700692354No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA7740.1646335627000755No Hit
CACACAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCGG7680.16335733353185786No Hit
CTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCT7670.16314462867048826No Hit
GTCTTCCAAATTACTTCCCACCCAGGTGGCCAGATTCATCAACTCACCCC7580.1612302849181618No Hit
GGATAATACGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT7570.1610175800567922No Hit
TATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATG7460.15867782658172652No Hit
GAAAAGAGGGAGTTTGCCAGTCAGGATTAAAGACAGGTACAGTAGAAGAA7130.1516585661565295No Hit
CCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCC7130.1516585661565295No Hit
CCCACGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACC7020.14931881268146383No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC6890.14655364948365895No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG6830.1452774203154413No Hit
ATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC6740.14336307656311484No Hit
GTATTATCCTGAACATACAGTTAATCATTACTTCCAAACTAGACACTATT6730.14315037170174524No Hit
GTTCAGGATAATACGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA6690.1422995522562668No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC6620.14081061822667956No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG6600.14038520850394037No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCTAGAAC6590.14017250364257075No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT6510.13847086475161388No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6490.1380454550288747No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC6480.13783275016750507No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACGATATGG6460.13740734044476588No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT6420.13655652099928745No Hit
ACACACAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCG6380.135705701553809No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC6260.13315324321737373No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG6130.13038808001956884No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6110.12996267029682962No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGAACGG6070.1291118508513512No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTCAACTTCAT5960.12677209737628553No Hit
ATGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGGAT5890.12528316334669828No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA5880.12507045848532866No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC5860.12464504876258946No Hit
CCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTTGA5820.12379422931711104No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT5770.12273070501026301No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5670.12060365639656695No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5570.11847660778287088No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCATCTCG5510.11720037861465324No Hit
GGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAG5440.11571144458506599No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5440.11571144458506599No Hit
CTTTGGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTG5440.11571144458506599No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCG5400.11486062513958756No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5370.11422251055547876No Hit
GAGACGGGTCGTCCGCGGGATTCAGCGCCGACGGGACGTAGACAAAGGAC5360.11400980569410914No Hit
GTAACCTATGTCAATGTTAACATGGGCCTAAAATTCAGACAACTATTGTG5320.11315898624863072No Hit
ATATAAGAGAGAAACTACACGCAGTGCCTCATTTTGTGGGTCACCATATT5260.11188275708041306No Hit
CTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTG5240.11145734735767386No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5210.11081923277356505No Hit
GGAACATATTGTGCAAAAATTGAAACAATGTTTTCGGAAACTTCCTATAA5150.10954300360534741No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5120.10890488902123859No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCATCTCGGGGCCG5050.10741595499165132No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA5040.10720325013028173No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGAACCATGGCTGCTAGGCTG5000.10635243068480331No Hit
ATCCTGAACATACAGTTAATCATTACTTCCAAACTAGACACTATTTACAT5000.10635243068480331No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4990.10613972582343369No Hit
TATCGAAAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA4950.10528890637795527No Hit
GCATTAATGCCTTTATATGCATGTATACAAGCTAAGCAGGCTTTCACTTT4920.10465079179384645No Hit
GCCATAAAAGTTCCACGCATGCGCTGATGGCCTAAGGCCAAGCCCCAACC4890.10401267720973761No Hit
ACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAACTGTAAGA4840.1029491529028896No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA4790.10188562859604156No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4790.10188562859604156No Hit
CTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGAC4770.10146021887330234No Hit
CTCTTATATAAAATACCAGCCTTCCACAAAGTATGTAAATAGTGTCTAGT4720.10039669456645432No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)