FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470135
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA26630.5664330458272624No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA24360.5181490422963617No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC21860.46497282695396003No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC19570.41626341370032016No Hit
CTTTCGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTG18270.3886117817222713No Hit
GCCTAGTCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC15870.33756261499356566No Hit
TATCCAAAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA15330.32607655247960693No Hit
ACCCATCTCGGGGCCGTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCG14290.30395524689716785No Hit
GTATGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG14100.29991385453114533No Hit
ATTTACATACTTTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACA13980.29736139619471No Hit
CCTCTCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13490.2869388579875993No Hit
TATCAAAAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA12780.2718368128303572No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA12400.2637540280983122No Hit
CTTTTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTG12260.26077616003913767No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGT11460.24375977112956917No Hit
AGCGAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCTAGAA10780.22929584055643593No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG10680.22716879194273984No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC10350.22014953151754282No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA9590.20398396205345273No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCGTAT9530.2027077328852351No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT9250.1967519967668861No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTTGA9120.19398683356908122No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG8920.18973273634168908No Hit
GTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC8770.186542163421145No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA8740.18590404883703618No Hit
GACTTTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTGC8710.18526593425292737No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG8660.1842024099460793No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA8590.1827134759164921No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG8510.18101183702553522No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA8460.1799483127186872No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCAACCCAAAAGACTGCCGTG8410.17888478841183914No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG8380.17824667382773032No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGC8170.17377987173896858No Hit
GATTTGGAACAGAAAGGTTCGTCCCCATGCCTTGTCGAGGTTTGGAAGAC8150.17335446201622937No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT8120.17271634743212055No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7890.1678241356206196No Hit
GGATAATACGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT7870.1673987258978804No Hit
ATTGTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA7860.1671860210365108No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG7850.1669733161751412No Hit
CTATTTACATACTTTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTA7820.16633520159103238No Hit
GTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT7720.16420815297733632No Hit
GTCTTCCAAATTACTTCCCACCCAGGTGGCCAGATTCATCAACTCACCCC7680.16335733353185786No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCATCTCGGGGCCG7630.16229380922500983No Hit
GCACTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGGAATTCCA7620.16208110436364023No Hit
GTTCCCAATCCTCTGGGATTCTTTCCCGGTCACCAGTTGGACCCAGCGTT7550.16059217033405299No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA7490.15931594116583536No Hit
CCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCC7350.15633807310666087No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCATCTCG7320.15569995852255203No Hit
CTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCT7220.15357290990885597No Hit
CACACAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCGG7090.1508077467110511No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT6570.13974709391983153No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT6490.1380454550288747No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCTAGAAC6470.13762004530613547No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC6460.13740734044476588No Hit
GTATTATCCTGAACATACAGTTAATCATTACTTCCAAACTAGACACTATT6460.13740734044476588No Hit
GAAAAGAGGGAGTTTGCCAGTCAGGATTAAAGACAGGTACAGTAGAAGAA6330.13464217724696098No Hit
ATGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGGAT6320.1344294723855914No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6290.13379135780148255No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG6290.13379135780148255No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA6250.1329405383560041No Hit
GAGACGGGTCGTCCGCGGGATTCAGCGCCGACGGGACGTAGACAAAGGAC6190.1316643091877865No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG6180.1314516043264169No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6090.12953726057409043No Hit
GTTCAGGATAATACGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA6080.12932455571272083No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT6070.1291118508513512No Hit
ACACACAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCG6000.12762291682176394No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC5940.1263466876535463No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT5930.12613398279217672No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGAACCATGGCTGCTAGGCTG5920.1259212779308071No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTCAACTTCAT5820.12379422931711104No Hit
CTTTTATGGCTCCTCTGCCGATCCATACTGCGGAACTCCTAGCAGCTTGT5790.12315611473300223No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5750.1223052952875238No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC5660.12039095153519733No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5650.12017824667382773No Hit
CTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTG5600.11911472236697969No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACGATATGG5590.1189020175056101No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5540.11783849319876205No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGACCGACCACG5480.11656226403054441No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5290.1125208716645219No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5280.11230816680315228No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA5280.11230816680315228No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5240.11145734735767386No Hit
ATCCTGAACATACAGTTAATCATTACTTCCAAACTAGACACTATTTACAT5210.11081923277356505No Hit
CTTTGGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTG5210.11081923277356505No Hit
CTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGAC5190.11039382305082583No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5130.10911759388260818No Hit
GCATTAATGCCTTTATATGCATGTATACAAGCTAAGCAGGCTTTCACTTT5080.10805406957576015No Hit
GTAACCTATGTCAATGTTAACATGGGCCTAAAATTCAGACAACTATTGTG5010.1065651355461729No Hit
GGAACATATTGTGCAAAAATTGAAACAATGTTTTCGGAAACTTCCTATAA4990.10613972582343369No Hit
CTTATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCA4950.10528890637795527No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGCACAATATGTT4900.10422538207110724No Hit
TATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATG4820.10252374318015038No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA4800.10209833345741118No Hit
TCTCGGGGCCGTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCG4760.10124751401193274No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC4740.10082210428919354No Hit
TATCGAAAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA4730.10060939942782392No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC4710.1001839897050847No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)