FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341690
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12730.37255992273698385No Hit
TCTTTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG12430.3637800345342269No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12420.363487371594135No Hit
ACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAG9610.28124908542831223No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG9160.2680792531241769No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG8670.25373876905967396No Hit
GTTGTTAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG8220.24056893675553867No Hit
ATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG8190.23969094793526297No Hit
GTACACCATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC7970.23325236325324122No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGAATTAAACCTTAT7840.2294477450320466No Hit
ATTAACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7790.2279844303315871No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7280.2130586203869004No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7210.2110099798062571No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7050.20632737276478677No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA6990.20457139512423542No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA6800.19901079926248938No Hit
GGCATAGACCTGACCGTTGCCGGGCAACGGGGTAAAGGTGCAGATATTGT6670.19520618104129475No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6610.19345020340074337No Hit
ATCCCGATTGGGACTTCAACCCCAACAAGGATCAATGGCCAGAGGCAAAT6590.19286487752055959No Hit
ACCAAAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAG6450.18876759635927304No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6330.18525564107817027No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC6150.17998770815651613No Hit
GGTCCTAGGAATCCTGATGTTGTGCTCTCCATGTTCGGTGCAGGGTCCCC6120.17910971933624045No Hit
CTCTTTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA6110.17881705639614856No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6010.17589042699522958No Hit
GGTCTCTGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGCTTGGC5740.1679885276127484No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT5620.16447657233164564No Hit
CTATCACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA5590.16359858351136994No Hit
CTATTAATAATGTCCTCCTTTAAATGAATGTTAGGAAAGGAGGGAGTTTG5590.16359858351136994No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTTGTTGACATTGCTGAGAGTCCAA5270.15423336942842927No Hit
CATTCTGTGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGAGG5240.1533553806081536No Hit
CTGTAAGAGGGCCCACGTATTGTTGACATCTATTAATAATGTCCTCCTTT5190.1518920659076941No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5050.14779478474640756No Hit
AATCTGGATTGTTTGAGTTGGCTCCGAACGCAGGGTCCAACTGGTGATCG4890.14311217770493723No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4840.14164886300447774No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGTTACTCCCTTAACTTCAT4790.14018554830401825No Hit
GGACTCGTGCAGGTCTTGCATGGCCCCGTGCTGGTAGTTGATGTTCCTGG4760.13930755948374257No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4740.1387222336035588No Hit
TGTATACACTCTAAGCAAGCTTTCACTTTCTCGCCAACTTACAAGGCCTT4700.1375515818431912No Hit
CCTTTAAATGAATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG4650.1360882671427317No Hit
CATGTATACACTCTAAGCAAGCTTTCACTTTCTCGCCAACTTACAAGGCC4550.13316163774181275No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG4520.13228364892153707No Hit
CGATAAGGTTCGCTCCAGACCGGCTGCGAGCAAAACACGCTGCTAGGAGT4470.13082033422107758No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC4430.12964968246071No Hit
TTATTAATAGATGTCAACAATACGTGGGCCCTCTTACAGTTAATGAAAAA4400.1287716936404343No Hit
GACCAGACCGTTGCCGGGCAACGGGGTAAAGGTGCAGATATTGTTTACAC4370.12789370482015863No Hit
GCATAGACCTGACCGTTGCCGGGCAACGGGGTAAAGGTGCAGATATTGTT4210.1232110977786883No Hit
GACCTGACCGTTGCCGGGCAACGGGGTAAAGGTGCAGATATTGTTTACAC4200.1229184348385964No Hit
TGGTAATAGAGGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCC4190.1226257718985045No Hit
TAATTATGCCTGCTAGGTTCTATCCTAACCTTACCAAATATTTGCCCTTG4170.1220404460183207No Hit
GTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAA4120.12057713131786121No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4050.11852849073721795No Hit
CTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA4040.11823582779712605No Hit
GGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTG4040.11823582779712605No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA4010.11735783897685037No Hit
ATACACTCTAAGCAAGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCT4000.11706517603675846No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA3990.11677251309666657No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3940.11530919839620708No Hit
TCAACTACCAGCACGGGGCCATGCAAGACCTGCACGAGTCCTGCTCAAGG3930.1150165354561152No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG3820.11179724311510433No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC3790.11091925429482866No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA3760.11004126547455295No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA3740.10945593959436917No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3700.10828528783400157No Hit
ACCTCATCGGGACTGACAACTCCGTTGTCCTCTCTCGGAAATACACCTCC3650.10682197313354211No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC3650.10682197313354211No Hit
GATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAA3600.10535865843308262No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT3560.10418800667271504No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC3550.10389534373262314No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC3490.10213936609207176No Hit
GGGTCCTAGGAATCCTGATGTTGTGCTCTCCATGTTCGGTGCAGGGTCCC3480.10184670315197986No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCGGTAAAGTTTCCCAC3470.10155404021188798No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG3470.10155404021188798No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA3450.10096871433170418No Hit
GTAAATAGACCGATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTT3450.10096871433170418No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)