FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002488314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135017
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6480.4799395631661198No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6450.4777176207440545No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6080.4503136642052482No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5510.4080967581860062No Hit
GTGTAAAAGGAGCAGCAAAGCCCAAAAGGCCCACAATTCTTTGACATACT3980.2947776946606724No Hit
GTACAAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT3570.264411148225779No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT3310.24515431390121242No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG3160.23404460179088557No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG3060.2266381270506677No Hit
TTGTTCACATCTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGG2880.21330647251827545No Hit
GTTTTAGTACAATATGTTCCTGCGGTAAAGTCCCCCAACTTCCAATTACA2800.20738129272610115No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGGACTTTACCGCAG2770.20515935030403581No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG2720.20145611293392682No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT2720.20145611293392682No Hit
ATGTCAACAATATGTGGGCCCTCTTACAATTAATGAAAAAAGGAGATTAA2660.1970122280897961No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTG2610.19330899071968716No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC2580.1910870482976218No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC2540.18812445840153463No Hit
CTATATAAGAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCATA2360.17479280386914242No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCGTAT2350.17405215639512062No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT2350.17405215639512062No Hit
CCTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTAT2330.17257086144707703No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGT2300.17034891902501167No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA2250.1666456816549027No Hit
CCTAATCTCCTCCCCTAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA2190.162201796810772No Hit
ATATAAGAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCATATT2160.15997985438870663No Hit
GGGCACATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATC2110.15627661701859766No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT2110.15627661701859766No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA2100.15553596954457588No Hit
GGACGTGTATTTCCGAGAGAGGACAACAGAGTTGTCGGTTCCGATAAGTT2060.15257337964848872No Hit
CCCCTAACTCCTCCCAGTCTTTAAACACACAGTCTTTGAAGTATGCCTCA2050.1518327321744669No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT2040.1510920847004451No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA2040.1510920847004451No Hit
CACACAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCGG2010.14887014227837975No Hit
GTTTTTGTACAATATGTTCCTGCGGTAAAGTCCCCCAACTTCCAATTACA2010.14887014227837975No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA1980.1466481998563144No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC1960.1451669049082708No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA1950.14442625743424903No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC1940.14368560996022722No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT1900.14072302006414006No Hit
GGAATCGTGCAGGTCTTGCATGGCCCCGTGCTGGTGGTTGATGTTCCTGG1870.1385010776420747No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGAGCAGCA1820.13479784027196573No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC1800.13331654532392218No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG1790.13257589784990037No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG1780.1318352503758786No Hit
GTTGACATCTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAG1780.1318352503758786No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA1770.13109460290185682No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC1720.12739136553174785No Hit
GATGTGAACAATATGTGGGCCCTCTTACAATTAATGAAAAAAGGAGATTA1710.12665071805772607No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG1700.12591007058370426No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA1660.1229474806876171No Hit
GTTCACATCTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAG1650.12220683321359532No Hit
ATATGTTCCTGCGGTAAAGTCCCCCAACTTCCAATTACATATCCCATAAA1620.11998489079152995No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC1600.11850359584348637No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA1590.11776294836946459No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC1580.11702230089544279No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC1580.11702230089544279No Hit
ACACACAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCG1570.11628165342142101No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT1570.11628165342142101No Hit
CCTACTCTCCTCCCCTAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA1560.1155410059473992No Hit
GAATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA1550.11480035847337743No Hit
ACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGAC1530.11331906352533384No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT1510.11183776857729028No Hit
CATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCCGGTCTGGAGC1490.11035647362924669No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC1480.10961582615522489No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA1470.10887517868120311No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC1470.10887517868120311No Hit
GGATAGCCACATTGTGTAAAAGGAGCAGCAAAGCCCAAAAGGCCCACAAT1470.10887517868120311No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC1460.1081345312071813No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC1450.10739388373315953No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG1440.10665323625913772No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATAC1430.10591258878511595No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA1430.10591258878511595No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC1420.10517194131109416No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG1410.10443129383707238No Hit
GCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCTGACC1410.10443129383707238No Hit
CCTTTATATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAAC1390.1029499988890288No Hit
GGAAAATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT1380.10220935141500699No Hit
ATTGTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA1370.10146870394098521No Hit
CCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTCAAGATG1370.10146870394098521No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA1370.10146870394098521No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA1360.10072805646696341No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)