FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219472
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCACCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13200.6014434643143545No Hit
ACTGAGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTCCG11580.527629948239411No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGCTCACCCGTG10840.49391266311875776No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10210.46520740686739087No Hit
GGCTATAGGAGTGCGAATCCACACTCCAAAGGAGACCAAATACTCAAGGA9480.4319457607348545No Hit
GTATATGAACCTTTACCCCGTTGCCCGACAACGGCCTGGTCTGTGCCAAG7630.3476525479332216No Hit
GGGTGGAGGAGTGCGAATCCACACTCCAAAGGAGACCAAATACTCAAGGA7580.34537435299263686No Hit
GTTCATATACTGTGTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA7230.3294269884085441No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTCCG6960.3171247357293869No Hit
CCTATAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6370.2902420354304877No Hit
GTGTAAATGGAGCGGCAAAGCCCAAAAGACCTACAATTCTTTGACATACT5710.26016986221477No Hit
CAAAACAACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGG5600.2551578333454837No Hit
GTTGCCTTGAGCAGGAGTCGTGCAGGTTTTGCAGGGTCCCGTGCTGGTTG5540.2524239994167821No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA5440.24786760953561274No Hit
CTCCAAAGGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG5290.2410330247138587No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA5230.2382991907851571No Hit
ATATGATCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA5080.2314646059634031No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC4860.2214405482248305No Hit
CCTATTAATAATGTCCTCATGTAAATGGATTTTAGGAAAGGAAGGAGTTT4730.21551724137931033No Hit
GTAGAGGAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGAAT4730.21551724137931033No Hit
GGATCATATTGTACAAAAAATCAAACACTGTTTTAGAAAACTTCCTGTTA4600.20959393453379016No Hit
CCATTTACACAATGTGGTTATCCTGCCTTAATGCCTTTGTATGCATGTAT4600.20959393453379016No Hit
CCCCAATACACCTCCGTGTGGGGGAGTGAACCCTGGGCCAAATGCTCCCG4550.20731573959320554No Hit
ATCATGGGAGGTTGGTCATCAAAACCTCGCAAAGGCATGGGGACGAATCT4540.20686010060508858No Hit
CATCATGGGAGGTTGGTCATCAAAACCTCGCAAAGGCATGGGGACGAATC4520.2059488226288547No Hit
GGTTCATATACTGTGTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA4380.1995698767952176No Hit
GCTCTGCAAGATCCCAGAGTCAGGGGTCTTCCTGCTGGTGGCTCCAGTTC4380.1995698767952176No Hit
CCTTTGGAGTGTGGATTCGCACTCCTCCACCCTATAGACCACCAAATGCC4260.19410220893781438No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGATGGC4190.19091273602099584No Hit
CCCCTGATGTAGCTCTTGTTCCCAAGAATATGGTGACCCGCAAAATGAGG4000.18225559524677407No Hit
CTTATATAGAATCCCAGCCTTCCAGAGAGTATGTAAATAATGTCGGGTCT3910.17815484435372164No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA3840.1749653714369031No Hit
ATTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAGAG3840.1749653714369031No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACAG3800.17314281548443536No Hit
ATTTAGTACTGTTAGGATAAAATCTAGCAGGCATAATTAATTTCAATCTT3790.17268717649631843No Hit
GTATTGGGGTGGAGCCCTCAGGCTCAGGGCACATTGGCTACTTTGCCAGC3770.17177589852008457No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATAACCACATTGTGTAAAT3710.16904206459138296No Hit
GTACTAAATATTTGCCTCTAGACAAAGGAATTAAACCTTATTATCCAGAT3700.168586425603266No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT3530.16084056280527811No Hit
ACTTGATACAGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGAGC3520.16038492381716118No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA3460.15765108988845958No Hit
GTACGGCAGACGGAGAAGGGGACGATAGAGTCCCAAGCGGCCCCTGGAGG3420.15582853393599183No Hit
CTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGATGGCTT3400.15491725595975794No Hit
CTATAGGGTGGAGGAGTGCGAATCCACACTCCAAAGGAGACCAAATACTC3350.1526390610191733No Hit
ATATGAACCTTTACCCCGTTGCCCGACAACGGCCTGGTCTGTGCCAAGTG3240.147627032149887No Hit
ACCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTA3210.1462601151855362No Hit
GATTAACGAGATGTGAGAGGCAATATTTGGAACAGGGTTGAGTGTTCCTG3190.14534883720930233No Hit
TCCCCTGATGTAGCTCTTGTTCCCAAGAATATGGTGACCCGCAAAATGAG3170.14443755923306847No Hit
TTATTATCCAGATCAAGTAGTTAATCATTACTTCCAGACCCGACATTATT3080.14033680834001605No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGATGGCTTTGGGGCATGGACATTGA3080.14033680834001605No Hit
CATGTATACAAGCGAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC3040.1385142523875483No Hit
GCCTATAGCCAAGCCCCAGCCAGTGGGAGTTGCGTCAGCAAACACTTGGC3030.13805861339943135No Hit
GGTGTATTGGGGTGGAGCCCTCAGGCTCAGGGCACATTGGCTACTTTGCC3020.13760297441131444No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACAGCACTCAGGCA3010.1371473354231975No Hit
CCTCTAGACAAAGGAATTAAACCTTATTATCCAGATCAAGTAGTTAATCA2980.1357804184588467No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC2960.1348691404826128No Hit
TTCCTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTC2870.1307683895895604No Hit
ATACAGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTAATGACTCTA2860.13031275060144346No Hit
GTCCCGTGCTGGTTGTTGTGGATCCTGGAATTAGAGGACAAACGGGCAAC2850.12985711161332653No Hit
GCCTAATCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGC2830.12894583363709267No Hit
TACCCTGGTAATCCAGAAGAACCAATAAGAAGATGAGGCATAGCAGCAGG2790.12712327768462492No Hit
CTCCAGGGGCCGCTTGGGACTCTATCGTCCCCTTCTCCGTCTGCCGTACC2770.12621199970839106No Hit
ACATAAACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAA2750.12530072173215717No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAGAGGCAAATATTTAGTACTGTT2720.12393380476780637No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCAGATGGCC2710.12347816577968944No Hit
ACCTAGCAGCCATGGAAATGATGTATATTTCCGAGAAAGGACGACAGAAT2680.12211124881533864No Hit
ACTTTATGGCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTA2640.12028869286287089No Hit
GTCTAGAGGCAAATATTTAGTACTGTTAGGATAAAATCTAGCAGGCATAA2640.12028869286287089No Hit
ATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATATT2620.11937741488663703No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGAGG2590.11801049792228621No Hit
GCTATAGGCCATCTGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGAT2540.11573230298170155No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCCTCTACTG2460.11208719107676605No Hit
GACAAGATGTGAGAGGCAATATTTGGAACAGGGTTGAGTGTTCCTGAACT2440.1111759131005322No Hit
ATATGCACCTTTACCCCGTTGCCCGACAACGGCCTGGTCTGTGCCAAGTG2430.11072027411241525No Hit
CTGCCGTACCGTCCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCC2420.11026463512429832No Hit
CTCTGGAAGGCTGGGATTCTATATAAGAGGGAAACTACACGTAGCGCCTC2410.10980899613618138No Hit
GGGTTGAGTGTTCCTGAACTGGAGCCACCAGCAGGAAGACCCCTGACTCT2400.10935335714806445No Hit
GCTTCGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGT2390.10889771815994752No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG2380.10844207917183057No Hit
CTTTATGGCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAG2370.10798644018371363No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG2360.10753080119559671No Hit
GGATTAAAGATAGGGACAGTAGAGGAATAAAGCCCAGTAAAATTTCCCAC2350.10707516220747977No Hit
TACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAA2340.10661952321936283No Hit
CCAATACACCTCCGTGTGGGGGAGTGAACCCTGGGCCAAATGCTCCCGCT2340.10661952321936283No Hit
TCACCATACAGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTAATGA2330.10616388423124591No Hit
ATTTACATACTCTCTGGAAGGCTGGGATTCTATATAAGAGGGAAACTACA2320.10570824524312897No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT2280.10388568929066122No Hit
GAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGAATACTAAC2260.10297441131442735No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT2250.10251877232631043No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG2240.10206313333819349No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)