FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences668858
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA26700.3991878694730421No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA25660.3836389786770884No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT24160.36121269387523214No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC22810.34102903755356145No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG21150.3162106157061738No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG20740.3100807645269998No Hit
GTGTAAAAGGGGCTGCAAACCCCAAAAGACCCACAATTCGTTGACATACT18790.28092659428458655No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC17540.262238023616373No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGA16210.24235338442539375No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT15400.23024319063239132No Hit
GATGTATACTTGCGGGAAAGCACGACAGAATTGTCAGTCCCGATGAGTTT15350.2294956478056628No Hit
ACCTTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATT15240.22785105358686No Hit
GTATACATCATTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC15050.2250103908452915No Hit
CATTAGGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTGTTTTCA14770.22082415101561167No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC14400.21529233409782045No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG14050.21005953431072066No Hit
ATATGTTCCTGTGGTAATGTGCCCCAACTCCCAATTACATATCCCATGAA13920.20811592296122644No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA13810.20647132874242363No Hit
CATTTAAACCCTCACAAAACAAAAAGGTGGGGATATTCCCTTAACTTCAT13020.19466015208011267No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC12910.19301555786130986No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG12860.19226801503458132No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA12550.18763324950886437No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA12520.18718472381282725No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA12480.1865866895514444No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA11810.17656961567328192No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA11640.17402797006240486No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC11300.16894467884065079No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG11300.16894467884065079No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC11250.16819713601392225No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT11200.16744959318719368No Hit
ATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTAT11190.16730008462184798No Hit
CATTGGGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTGTTTTCA11090.1658049989683909No Hit
GCCTAGCAGCCATGGGAATGATGTATACTTGCGGGAAAGCACGACAGAAT11080.16565549040304517No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC10970.1640108961842424No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTGTCGAAGACTGGGGA10800.16146925057336536No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG10560.15788104500506836No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT10390.15533939939419128No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATTTAGCCACCTGGGTGGGAAGTAA10300.1539938223060799No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG10260.15339578804469708No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC9890.14786397112690586No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA9890.14786397112690586No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGGAAGT9670.14457478268930027No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA9650.14427576555860885No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC9590.1433787141665346No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC9580.14322920560118887No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC9560.14293018847049746No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT9460.1414351028170404No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA9330.1394914914675462No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC9080.13575377733390345No Hit
GTGGTAATGTGCCCCAACTCCCAATTACATATCCCATGAAGTTAAGGGAA8950.13381016598440926No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA8910.1332121317230264No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCATGCACGCGCTGATGGCC8900.13306262315768072No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATTTAGCCACCTGGGTGGGAAGT8790.1314180289388779No Hit
GGTTCCATGCACGCGCTGATGGCCTATGGCCAAGCCCCAACCAGTGGGGG8780.1312685203735322No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG8780.1312685203735322No Hit
ATGTAATTGGGAGTTGGGGCACATTACCACAGGAACATATTGTACAAAAA8770.1311190118081865No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCACCAACTTACAAGGCC8690.12992294328542084No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA8660.12947441758938372No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCTGGTCTGTGCCAAG8660.12947441758938372No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT8610.12872687476265515No Hit
CCCCAACAGTCCCCCATGGGGAGGGATGAACCCTGGCCCGAATGCTCCCA8600.12857736619730945No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTGTCGAAGACTGGGGACCCTG8430.1260357205864324No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC8430.1260357205864324No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG8390.12543768632504956No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC8330.12454063493297532No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG8330.12454063493297532No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA8280.12379309210624678No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC8190.12244751501813539No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCTGGTCTGTGCCAAGTG8100.12110193793002401No Hit
ATGTATACTTGCGGGAAAGCACGACAGAATTGTCAGTCCCGATGAGTTTT8100.12110193793002401No Hit
GACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT8090.12095242936467832No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG8070.12065341223398689No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG7990.11945734371122121No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT7930.11856029231914697No Hit
CAAATCTTTCTGTCCCCAATCCCCTGGGATTCTTCCCCGATCATCAGTTG7920.11841078375380125No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT7920.11841078375380125No Hit
TATCCACATTGTGTAAAAGGGGCTGCAAACCCCAAAAGACCCACAATTCG7820.11691569810034416No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA7660.11452356105481282No Hit
AGTATACATCATTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG7660.11452356105481282No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCTGGTCTGTGCCAAGTGTTTGCTG7640.11422454392412142No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG7610.11377601822808428No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT7550.11287896683601004No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC7530.11257994970531862No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT7500.1121314240092815No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTACCACAG7490.11198191544393578No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT7430.11108486405186153No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG7430.11108486405186153No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA7400.11063633835582441No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCACCTTTTTGTTTTG7390.11048682979047869No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCCCGGGGCCG7370.11018781265978728No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG7360.11003830409444157No Hit
CATTACCACAGGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAA7330.10958977839840445No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA7180.10734714991821881No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA7120.10645009852614457No Hit
TCTGTGGAGTTACTCTCTTTTTTACCTTCTGACTTCTTTCCTTCTATTCG7080.10585206426476172No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG7080.10585206426476172No Hit
ATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCT7010.10480550430734177No Hit
GGATATCCACATTGTGTAAAAGGGGCTGCAAACCCCAAAAGACCCACAAT7000.10465599574199606No Hit
GATCTACAGCATGGGAGGTTGGTCTTCCAAACCTCGAAAAGGCATGGGGA6970.10420747004595893No Hit
GTACAGGGTCCCCAGTCTTCGACAAGATTGACGATATGGCAGAGACAGTA6970.10420747004595893No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGAAGAGGC6960.10405796148061322No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAAGGGGCTGCA6940.1037589443499218No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT6830.10211435013111902No Hit
CCATATTCTTGGGAACAAGATCTACAGCATGGGAGGTTGGTCTTCCAAAC6810.1018153330004276No Hit
ATCCTAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTAT6760.10106779017369906No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCACCTTTTTGTTT6750.10091828160835334No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC6730.10061926447766192No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)