FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401149
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAACAAC23290.5805822774081452No Hit
TATGCAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATG14130.35223819578261445No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC13470.33578545627684475No Hit
CTAAAGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTC11800.2941550396486094No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA11780.29365647178479815No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA11260.2806937073257069No Hit
TTTTATGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGGCAGA11230.2799458555299901No Hit
GTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT10640.26523810354755967No Hit
ATGCAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC9600.2393125746293771No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG9270.23108620487649226No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC9170.22859336555743626No Hit
GGGCATTAGAGCAGGATAACCACATTGTGTAAAAGGAGCAGCAAAACCCA9120.22734694589790827No Hit
GTGAAAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAA8940.22285983512360746No Hit
CTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA8460.2108942063921386No Hit
ACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAA7780.1939428990225577No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7440.18546724533776726No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACT7110.17724087558488244No Hit
GGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGG7030.1752466041296376No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6990.17424946840201522No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA6850.17075949335533677No Hit
GAGTATCCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA6840.1705102094234312No Hit
GAGAAACACTCATCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACC6730.16776808617246958No Hit
GTACCAAACCTTCGGACGGAAATTGCACTTGTATTCCCATCCCATCATCT6710.16726951830865838No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6700.16702023437675279No Hit
ACACATAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAAGAGCT6690.1667709504448472No Hit
GGGACCTAGTAGTCAGTTATGTTAACACTAATGTGGGCCTAAAGTTCAGG6670.166272382581036No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6630.16527524685341358No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA6590.16427811112579116No Hit
CGTTCTTGCCTTCTGACTTCTTTCCTTCAGTACGAGATCTTCTAGATACC6520.16253312360245195No Hit
GACTTCGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG6450.16078813607911274No Hit
GTTATGGCTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT6410.15979100035149033No Hit
CTAATGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTC6370.15879386462386794No Hit
GGGTTACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT6370.15879386462386794No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC6340.15804601282815114No Hit
TTTCCGGCTCCTCTGCCGATCCATACTGCGGAACTCCTAGCCGCTTGTTT6170.15380818598575594No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT6170.15380818598575594No Hit
GTGTCTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCTTCCTGACTGGCG6160.1535589020538503No Hit
GGATAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAAT6160.1535589020538503No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6100.1520631984624167No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5950.14832393948383268No Hit
CCTCAAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5950.14832393948383268No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5900.1470775198243047No Hit
ACTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAG5890.14682823589239907No Hit
AGAACAAGAACTCCCTCGCCTCGCAGACGAAGGTCTCAATCGCCGCGTCG5850.14583110016477668No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC5840.1455818162328711No Hit
GGGCTTTAGAGCAGGATAACCACATTGTGTAAAAGGAGCAGCAAAACCCA5800.14458468050524867No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG5800.14458468050524867No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC5770.14383682870953185No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG5730.14283969298190946No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC5710.14234112511809827No Hit
TTTTGTGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGGCAGA5710.14234112511809827No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5520.13760473041189183No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5470.13635831075236385No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC5470.13635831075236385No Hit
CATTTACACCAAGACATTATCAACAGATGTGAACAATTTGTAGGCCCACT5230.1303754963866294No Hit
GGCCTCTCACTCTGGGATCTTGCAGTGTTTGGTGGAAGGTTGTGGAGTTC5220.1301262124547238No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCGAAAACATTCTTTGATTTTCTGT5190.129378360659007No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT5180.1291290767271014No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG5150.1283812249313846No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5150.1283812249313846No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5080.1266362374080454No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5040.12563910168042297No Hit
TCTTCTTCTAGGGGACCTGCCTCGTCGTCTAACAACAGTAGTCTCCGGAA4970.12389411415708379No Hit
AGTCTAGACTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4880.12165055876993337No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC4870.12140127483802776No Hit
ACTGTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAG4800.11965628731468855No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4790.11940700338278296No Hit
GTGATAAGGCTTCCCGATATAGAGCTGAGGCGGTATCTAGAAGATCTCGT4770.11890843551897175No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT4770.11890843551897175No Hit
ATGGCTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGTTTT4770.11890843551897175No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT4740.11816058372325494No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4680.11666488013182133No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT4670.11641559619991575No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA4650.11591702833610452No Hit
CCTTTTACACAATGTGGTTATCCTGCTCTAAAGCCCTTGTATGCATGTAT4630.11541846047229333No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC4570.11392275688085973No Hit
AGCTTGAGGGGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA4560.11367347294895414No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACACTGCAAG4480.11167920149370932No Hit
CTTTGGGACATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA4480.11167920149370932No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATG4470.11142991756180373No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAAGAATTGGTGGGCGTTC4450.11093134969799251No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4450.11093134969799251No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4350.10843851037893651No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4310.10744137465131409No Hit
CCAACATCCAGGGACCTAGTAGTCAGTTATGTTAACACTAATGTGGGCCT4300.1071920907194085No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT4300.1071920907194085No Hit
GACCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCA4280.1066935228555973No Hit
GTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAATTTT4190.10444996746844688No Hit
TATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATG4190.10444996746844688No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGGAAAATTCCTATGGGAG4180.10420068353654127No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG4170.10395139960463569No Hit
CCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAAGTATGCCTCA4130.10295426387701327No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)