FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430116
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA31050.7218982786039115No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA30850.717248370207107No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAAAACAAATACC20600.47894056487087205No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC16900.39291725952998724No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG16390.38105999311813554No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15090.3508355885389058No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT13940.3240986152572794No Hit
ACACAAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTATA13260.3082889267081439No Hit
ACAGTAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTATA13240.3078239358684634No Hit
TCCAAAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA12760.2966641557161324No Hit
GACTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCT10910.25365250304569No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC10750.24993257632824634No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC10040.23342540151959004No Hit
GTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGACAGGCACA9910.23040296106166708No Hit
ACACATAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAAGAGCT9790.2276130160235843No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC9520.22133563968789816No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCACAGAGAGTCCAA9220.21436077709269127No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA8980.20878088701652575No Hit
TATCCGTCCCGAGAATATTTGCTCCAGACCTGCTGCGAGCAAAACAAGCG8920.20738591449748442No Hit
GTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT8540.19855108854355571No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT8190.19041374884914766No Hit
CCTCAAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8100.1883212900705856No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG8060.1873913083912247No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT7870.18297389541426035No Hit
GGTATATTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTC7810.18157892289521896No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7790.1811139320555385No Hit
TAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT7610.1769290144984144No Hit
CATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCTGGAGC7520.17483655571985232No Hit
CACTAATATGGGCCTAAAGTTCAGGCAATTATTGTGGTTTCACATTTCTT7360.17111662900240865No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATGTTGGATCTTC7210.16762919770480522No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6980.16228180304847994No Hit
GTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGTCTCAATCGC6950.16158431678895924No Hit
GTCCCGTGCTGGTGGTCGAAGATCCTGGAATTAGAGGACAGACGGGCAAC6900.16042183968975812No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6680.15530694045327306No Hit
GGACAGATAACTCTGTTGTTCTATCCCGCAAATATACATCGTTTCCATGG6650.15460945419375238No Hit
GACTTGTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCT6550.1522844999953501No Hit
TTCTTCTACTGTGCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC6540.15205200457550985No Hit
GGATACTGGATCTTCCAAATTACCACCCACCCAGGTGGCTAGAGTCATTA6520.15158701373582942No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG6500.15112202289614896No Hit
CTTTTACACAATGTGGATATCCTGCATTAATGCCCTTGTATGCATGTATT6490.1508895274763087No Hit
TGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT6470.15042453663662825No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6420.14926205953742713No Hit
ATGTGGATATCCTGCATTAATGCCCTTGTATGCATGTATTCAATCTAAGC6320.14693710533902482No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6240.14507714198030297No Hit
CTACTGTGGGCCTAAAGTTCAGGCAATTATTGTGGTTTCACATTTCTTGT6230.14484464656046275No Hit
GGGGTAGGCTGCCTTCCTGACTGGCGATTGGTAGAGGCAGGAGGCGGATT6220.14461215114062254No Hit
GGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGCCTAAAGTTCAGG6220.14461215114062254No Hit
GTTCTCTCCCGCAAATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGC6210.14437965572078232No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT6140.14275218778190069No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6140.14275218778190069No Hit
GTAGAAGCAGGAGGCGGATTTGTTGGCAATGTTTGTAGTATGCCCTGAGC6140.14275218778190069No Hit
ATCAAGGTATGTTGCCCGTCTGTCCTCTAATTCCAGGATCTTCGACCACC6130.14251969236206047No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6120.14228719694222025No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6070.14112471984301908No Hit
GCATTAAACCTTACTATCCAGAACATCTAGTTAATCATTACTTCCAAACC6060.14089222442317886No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG5950.13833477480493633No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5950.13833477480493633No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGGTACTCTTTACATTTCAT5920.13763728854541565No Hit
CTAGCCACCTGGGTGGGTGGTAATTTGGAAGATCCAACATCCAGGGACCT5850.13600982060653405No Hit
GAAAATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC5710.13275488472877087No Hit
AACAAGAGGGATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTG5640.13112741678988923No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5630.13089492137004902No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC5610.13042993053036855No Hit
GTATGGATCGGCAGAGGAGCCCGAAAGGTTCCACGCATGCGCTGATGGCC5550.12903495801132717No Hit
GACTTGTGTTGTTAGACGACGAGGCAGGTCCACTAGAAGAAGAACTCCCT5490.1276399854922858No Hit
GAGTACCCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA5460.12694249923276513No Hit
ATATTTGCTCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGC5440.12647750839308466No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5430.12624501297324442No Hit
CTCTTTGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC5410.12578002213356396No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG5360.12461754503436283No Hit
GTGGATATCCTGCATTAATGCCCTTGTATGCATGTATTCAATCTAAGCAG5350.1243850496145226No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTTAGACAAGAAATGTGAAACCACA5340.12415255419468237No Hit
TATCCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5300.12322257251532145No Hit
GTATCGGGAAGCCTTAGAGTCACCTGAGCATTGTTCTCCTCACCATACTG5280.12275758167564099No Hit
GCTCTGTATCGGGAAGCCTTAGAGTCACCTGAGCATTGTTCTCCTCACCA5250.1220600954161203No Hit
GGGTTAAAGACAGGCACAGTAGAAGAATAAAGCCCCGTAAAGTTTCCCAC5240.12182759999628007No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTC5230.12159510457643984No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGC5220.1213626091565996No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5180.12043262747723871No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5170.12020013205739848No Hit
CCGACTACTGTCTCTCACATATCGTCAATCTTCTCGAGGATTGGGGACCC5170.12020013205739848No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA5160.11996763663755824No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5160.11996763663755824No Hit
CTAATATGGGCCTAAAGTTCAGGCAATTATTGTGGTTTCACATTTCTTGT5150.11973514121771801No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5140.11950264579787778No Hit
CTACAAACATTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC5070.11787517785899618No Hit
CTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG5060.11764268243915595No Hit
GCCTTAGAGTCACCTGAGCATTGTTCTCCTCACCATACTGCACTCAGGCA4950.11508523282091343No Hit
GCCCACCCCTCTGTCTCCACCTTTGAGAAACACTCATCCTCAGGCCATGC4950.11508523282091343No Hit
GGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGG4940.1148527374010732No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4900.11392275572171227No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCACCTGA4880.11345776488203184No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG4870.11322526946219161No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC4860.11299277404235138No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA4820.11206279236299045No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT4820.11206279236299045No Hit
TATTTGCTCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCA4780.11113281068362954No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4770.11090031526378931No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCCAGCCCGAATGCTCCAG4760.11066781984394908No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG4760.11066781984394908No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4760.11066781984394908No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4740.11020282900426862No Hit
GTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGATCTTGTGGCAAT4690.10904035190506746No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT4690.10904035190506746No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4680.10880785648522726No Hit
CTGTCTGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGC4660.1083428656455468No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA4650.10811037022570656No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG4640.10787787480586633No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAATACTAAC4640.10787787480586633No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC4620.10741288396618587No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGATAGAACAACAGAGT4610.10718038854634564No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4560.1060179114471445No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT4550.10578541602730426No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG4540.10555292060746403No Hit
GGATGTTGGATCTTCCAAATTACCACCCACCCAGGTGGCTAGAGTCATTA4530.1053204251876238No Hit
GTTCTGGATAGTAAGGTTTAATGCCCTTATCCAATGGCAAATATTTGGTA4530.1053204251876238No Hit
ACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTCTCATTGACTGTGAGT4500.1046229389281031No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4450.10346046182890198No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA4450.10346046182890198No Hit
GACCAGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGTTTACAC4430.10299547098922152No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT4370.10160049847018014No Hit
GGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGTCTCAATCG4320.10043802137097899No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4320.10043802137097899No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4310.10020552595113875No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)