FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430116
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA33530.7795571427242883No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA28750.6684243320406588No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAAAACAAATACC19310.44894865571148246No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG16870.3922197732704666No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT16240.3775725618205321No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC14080.32735355113504266No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12270.28527188014396115No Hit
ACACAAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTATA11730.2727171274725888No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC10860.25249002594648884No Hit
TCCAAAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA10820.2515600442671279No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC10580.24598015419096245No Hit
GTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGACAGGCACA10500.2441201908322406No Hit
ACAGTAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTATA10460.24319020915287967No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG8980.20878088701652575No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA8960.2083158961768453No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA8770.20389848319988094No Hit
GACTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCT8380.19483116182611204No Hit
ACACATAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAAGAGCT7860.18274139999442013No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCACAGAGAGTCCAA7850.18250890457457988No Hit
GTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT7760.18041644579601782No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC7660.17809149159761553No Hit
TATCCGTCCCGAGAATATTTGCTCCAGACCTGCTGCGAGCAAAACAAGCG7440.1729765923611305No Hit
GCATTAAACCTTACTATCCAGAACATCTAGTTAATCATTACTTCCAAACC7400.17204661068176957No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT7340.17065163816272821No Hit
CCTCAAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7320.17018664732304775No Hit
GGTATATTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTC7220.16786169312464544No Hit
GTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGTCTCAATCGC7110.16530424350640294No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG7080.16460675724688223No Hit
GGACAGATAACTCTGTTGTTCTATCCCGCAAATATACATCGTTTCCATGG7070.164374261827042No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC7000.1627467938881604No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6950.16158431678895924No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT6830.15879437175087652No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6740.15670191297231445No Hit
GTTCTCTCCCGCAAATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGC6650.15460945419375238No Hit
CACTAATATGGGCCTAAAGTTCAGGCAATTATTGTGGTTTCACATTTCTT6510.15135451831598917No Hit
TAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT6410.1490295641175869No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6280.1460071236596639No Hit
GGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGCCTAAAGTTCAGG6230.14484464656046275No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT6220.14461215114062254No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATGTTGGATCTTC6180.1436821694612616No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5970.1387997656446168No Hit
CTTTTACACAATGTGGATATCCTGCATTAATGCCCTTGTATGCATGTATT5970.1387997656446168No Hit
TTCTTCTACTGTGCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC5950.13833477480493633No Hit
ATGTGGATATCCTGCATTAATGCCCTTGTATGCATGTATTCAATCTAAGC5900.1371722977057352No Hit
GTCCCGTGCTGGTGGTCGAAGATCCTGGAATTAGAGGACAGACGGGCAAC5840.1357773251866938No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG5750.13368486640813176No Hit
TGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT5730.1332198755684513No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG5690.1322898938890904No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5690.1322898938890904No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5510.12810497633196624No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGGTACTCTTTACATTTCAT5460.12694249923276513No Hit
GGATACTGGATCTTCCAAATTACCACCCACCCAGGTGGCTAGAGTCATTA5460.12694249923276513No Hit
CTAGCCACCTGGGTGGGTGGTAATTTGGAAGATCCAACATCCAGGGACCT5460.12694249923276513No Hit
GTATGGATCGGCAGAGGAGCCCGAAAGGTTCCACGCATGCGCTGATGGCC5420.1260125175534042No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5380.12508253587404328No Hit
CATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCTGGAGC5370.12485004045420306No Hit
ATCAAGGTATGTTGCCCGTCTGTCCTCTAATTCCAGGATCTTCGACCACC5360.12461754503436283No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5350.1243850496145226No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5290.12299007709548122No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5260.12229259083596053No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTTAGACAAGAAATGTGAAACCACA5230.12159510457643984No Hit
GACTTGTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCT5160.11996763663755824No Hit
ATATTTGCTCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGC5160.11996763663755824No Hit
GGGGTAGGCTGCCTTCCTGACTGGCGATTGGTAGAGGCAGGAGGCGGATT5150.11973514121771801No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG5130.11927015037803755No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT5070.11787517785899618No Hit
GCCTTAGAGTCACCTGAGCATTGTTCTCCTCACCATACTGCACTCAGGCA5040.11717769159947548No Hit
CTGTGATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT5010.11648020533995479No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT4930.11462024198123297No Hit
GGGTTAAAGACAGGCACAGTAGAAGAATAAAGCCCCGTAAAGTTTCCCAC4910.1141552511415525No Hit
GTTCTGGATAGTAAGGTTTAATGCCCTTATCCAATGGCAAATATTTGGTA4900.11392275572171227No Hit
GGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGG4870.11322526946219161No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCACCTGA4860.11299277404235138No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTC4830.11229528778283068No Hit
GTATCGGGAAGCCTTAGAGTCACCTGAGCATTGTTCTCCTCACCATACTG4810.11183029694315022No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4750.11043532442410885No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4740.11020282900426862No Hit
GCCCACCCCTCTGTCTCCACCTTTGAGAAACACTCATCCTCAGGCCATGC4740.11020282900426862No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGC4720.10973783816458815No Hit
CTCTTTGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC4710.10950534274474792No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA4690.10904035190506746No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG4690.10904035190506746No Hit
GTGGATATCCTGCATTAATGCCCTTGTATGCATGTATTCAATCTAAGCAG4680.10880785648522726No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4670.10857536106538702No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAATACTAAC4630.1076453793860261No Hit
CTACAAACATTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC4620.10741288396618587No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCCAGCCCGAATGCTCCAG4620.10741288396618587No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4620.10741288396618587No Hit
CTACTGTGGGCCTAAAGTTCAGGCAATTATTGTGGTTTCACATTTCTTGT4620.10741288396618587No Hit
GTAGAAGCAGGAGGCGGATTTGTTGGCAATGTTTGTAGTATGCCCTGAGC4600.1069478931265054No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4590.10671539770666517No Hit
GAAAATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC4560.1060179114471445No Hit
CTAATATGGGCCTAAAGTTCAGGCAATTATTGTGGTTTCACATTTCTTGT4540.10555292060746403No Hit
GAGTACCCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA4540.10555292060746403No Hit
GCTCTGTATCGGGAAGCCTTAGAGTCACCTGAGCATTGTTCTCCTCACCA4500.1046229389281031No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA4500.1046229389281031No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGT4500.1046229389281031No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4490.10439044350826288No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4490.10439044350826288No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4460.10369295724874221No Hit
CTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG4430.10299547098922152No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4410.10253048014954105No Hit
GACCAGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGTTTACAC4400.10229798472970082No Hit
CTACTGTGCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT4390.10206548930986059No Hit
GTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGATCTTGTGGCAAT4350.10113550763049968No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4340.10090301221065945No Hit
GACTTGTGTTGTTAGACGACGAGGCAGGTCCACTAGAAGAAGAACTCCCT4340.10090301221065945No Hit
CTATTACCGTTTCTCTTCCAAAAGTTAGACAAGAAATGTGAAACCACAAT4310.10020552595113875No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)