FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497635
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA25820.5188541802726898No Hit
GTGCTGGTGGACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC20550.41295326896219114No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA20370.40933616003697487No Hit
ATCTAAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG20070.4033076451616145No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT18540.3725622192972761No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT18490.37155746681804935No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC18300.36773940739698774No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG17280.3472424568207622No Hit
CATGTATACAGGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC17230.34623770434153545No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA16220.32594170426115526No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG14840.2982105358344972No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG14210.28555065459624024No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT13650.2742974268289007No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA13130.2638480010449426No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA13120.2636470505490972No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG12860.2584223376571182No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT12250.2461643574105519No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA12100.2431500999728717No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTGGTAACATT11750.23611683261828448No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG11080.22265314939664613No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC10980.22064364443819268No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA10840.2178303374963578No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA10540.2118018226209973No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA10280.20657710972901824No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCCGT10160.20416570377887405No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC9880.1985390898952043No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT9700.19492198096998803No Hit
GTCCGGTGCTGGTTGTTGAGGATCCTGGAATTAGAGGACAAACGGGCAAT9520.19130487204477176No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT9510.19110392154892641No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC9450.18989821857385433No Hit
CTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATAC9360.1880896641112462No Hit
GTATAGGGCCCTGACTCTGGGATCTTGAAGAGTTTGGTGGAAAGTGGTGG9040.18165924824419505No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT8760.17603263436052527No Hit
ATATTAACGTTGACATAGCCGACTACCAATTCCCTGGATGCTGGATCTTC8610.17301837692284505No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG8530.17141077295608226No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8350.16779366403086599No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG8210.1649803570890311No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA8170.1641765551056497No Hit
GTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCTTT8150.16377465411395903No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAACCTTATCGAAGACTGGGGA8050.16176514915550555No Hit
ATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCT8040.16156419865966018No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGAAAGA8010.16096134717212415No Hit
GAATACTGTCTCTGCCATATCGTCAACCTTATCGAAGACTGGGGACCCTG7980.1603584956845881No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG7960.1599565946928974No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT7890.15854994122197996No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA7860.1579470897344439No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAGGCTAAACAG7630.1533252283300009No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGAAT7590.1525214263466195No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACAAAATGCC7540.15151667386739276No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA7540.15151667386739276No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT7410.14890431742140323No Hit
GCCCTATACTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGC7400.14870336692555788No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC7400.14870336692555788No Hit
ATCAAGGTATATTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACAACC7330.14729671345464046No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG7190.14448340651280556No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGG7170.14408150552111487No Hit
GGTTTAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA7170.14408150552111487No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATTCCTATGGGAG7150.1436796045294242No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG7130.14327770353773348No Hit
GAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA7090.14247390155435208No Hit
CCCCAACAGTCCCCCATGGGGAGGGGTGAACCCTGGCCCGAATGCTCCCA7090.14247390155435208No Hit
CTGTTTCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATAC6980.14026344610005326No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6940.13945964411667186No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA6930.13925869362082652No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA6930.13925869362082652No Hit
GGAAATGTGAAACCACAATAGTTGTCTGAGTTTTAGGCCCATATTAACGT6910.1388567926291358No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT6880.13825394114159978No Hit
TTGTACACCTCACCATACGGCACTCAGGCAAGCTATTCTGTGTTGGGGTG6870.1380529906457544No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6810.13684728767068233No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTGGTA6770.13604348568730093No Hit
GTACAGGGTCCCCAGTCTTCGATAAGGTTGACGATATGGCAGAGACAGTA6760.1358425351914556No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT6750.13564158469561025No Hit
GTACAGGGCCCTGACTCTGGGATCTTGAAGAGTTTGGTGGAAAGTGGTGG6750.13564158469561025No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG6750.13564158469561025No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC6740.13544063419976488No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6640.1334311292413114No Hit
CCCTTACAGTGAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG6570.13202447577039397No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAATTCCAGATTGGGACCT6570.13202447577039397No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT6520.13101972329116723No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC6500.13061782229947652No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA6490.13041687180363118No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG6420.12901021833271373No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC6420.12901021833271373No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT6380.12820641634933233No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG6360.12780451535764165No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT6320.12700071337426025No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA6200.12458930742411606No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6190.12438835692827072No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACT6170.12398645593658002No Hit
GGCAATATACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAG6140.12338360444904399No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT6110.12278075296150792No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGGCACTCAGGCA6030.12117314899474516No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA5990.12036934701136374No Hit
CCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGAAAC5980.12016839651551842No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5980.12016839651551842No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT5970.11996744601967305No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5830.11715413907783817No Hit
TCTATCAACAATGTCCTCCCGTAAATGAATGTCAGGAAAAAAAGGAGTTT5820.11695318858199281No Hit
CACCTTTCCTGTCTTTCTTTTGGGAGAGAAACTGTTCTTGAATATTTGGT5800.11655128759030214No Hit
GTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAG5780.11614938659861145No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG5720.11494368362353934No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA5660.11373798064846724No Hit
CCTTATGTGTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCT5600.11253227767339517No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA5590.1123313271775498No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC5520.11092467370663237No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC5510.11072372321078704No Hit
CCCCATGGGGGACTGTTGGGGTGGAGCCCTCAGGCTCAGGGCCTACTCAC5500.11052277271494167No Hit
CTTCTGGACTATCAAGGTATATTGCCCGTTTGTCCTCTAATTCCAGGATC5480.11012087172325097No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5470.10991992122740563No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA5450.10951802023571493No Hit
AGATAAGGGAGTAGGCTGCCTTCCTGACTGCCGATTGGTGGAGGCAGGAG5440.10931706973986957No Hit
CTCTTATGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTCACGGTGGTCTC5430.10911611924402423No Hit
GATCTACAGCATGGGAGGTTGGTCTTCCAAACCTCGAAAAGGCATGGGGA5370.10791041626895213No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC5360.10770946577310679No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGTGTCCAAGGAATACTAAC5320.1069056637897254No Hit
CACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCT5310.10670471329388007No Hit
CCATATTCTTGGGAACAAGATCTACAGCATGGGAGGTTGGTCTTCCAAAC5300.1065037627980347No Hit
GGTCCTAGGAGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCC5300.1065037627980347No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAGGCTAAAC5260.1056999608146533No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGAACAG5220.10489615883127193No Hit
GTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC5210.10469520833542656No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGGGGTTT5190.10429330734373586No Hit
CTGTTGGGGTGGAGCCCTCAGGCTCAGGGCCTACTCACAACTGTGCCAGC5170.10389140635204516No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG5170.10389140635204516No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG5170.10389140635204516No Hit
GTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAAAT5130.1030876043686638No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG5120.10288665387281842No Hit
GTTTCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGG5090.10228380238528238No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5090.10228380238528238No Hit
GCTTTAATGCCTTTATATGCATGTATACAGGCTAAACAGGCTTTTACTTT5070.10188190139359168No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5060.10168095089774636No Hit
CCATACGGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTAATGAATC5050.10148000040190099No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAA5040.10127904990605566No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCGGTG5030.10107809941021029No Hit
GTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT5020.10087714891436494No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGAAAGACA5020.10087714891436494No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT4990.10027429742682889No Hit
CCTTCTTTTCCTGACATTCATTTACGGGAGGACATTGTTGATAGATGTAA4990.10027429742682889No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)