FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497635
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA23310.4684156058155074No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA19510.3920544173942749No Hit
ATCTAAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG19470.3912506154108935No Hit
GTGCTGGTGGACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC18580.3733660212806575No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT17770.35708903111718426No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG17150.34463010037477265No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT16270.32694645674038203No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC15920.3199131893857948No Hit
CATGTATACAGGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC15290.3072533081475379No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA14420.28977061500899254No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG13780.27690978327489024No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT13390.2690727139369216No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG13370.26867081294523093No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA12320.24757101088146938No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA12080.24274819898118097No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG12040.24194439699779957No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT11990.24093964451857283No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA11900.2391310900559647No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTGGTAACATT11370.22848071377616122No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA10670.21441417906698684No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCCGT10460.21019421865423452No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC10080.20255809981211131No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA9930.19954384237443104No Hit
GTCCGGTGCTGGTTGTTGAGGATCCTGGAATTAGAGGACAAACGGGCAAT9880.1985390898952043No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT9870.19833813939935896No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG9860.19813718890351362No Hit
CTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATAC9360.1880896641112462No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA9250.18587920865694735No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC9210.18507540667356598No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGAAAGA9150.18386970369849387No Hit
ATATTAACGTTGACATAGCCGACTACCAATTCCCTGGATGCTGGATCTTC8910.17904689179820552No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT8900.17884594130236017No Hit
GTATAGGGCCCTGACTCTGGGATCTTGAAGAGTTTGGTGGAAAGTGGTGG8480.17040602047685552No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC8310.1669898620474846No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACAAAATGCC8240.16558320857656716No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT8140.16357370361811369No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAACCTTATCGAAGACTGGGGA8020.1611622976679695No Hit
GTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCTTT7670.1541290303133823No Hit
GAATACTGTCTCTGCCATATCGTCAACCTTATCGAAGACTGGGGACCCTG7630.1533252283300009No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC7460.14990906990063No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT7360.1478995649421765No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA7330.14729671345464046No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGG7300.1466938619671044No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA7230.14528720849618695No Hit
CCCCAACAGTCCCCCATGGGGAGGGGTGAACCCTGGCCCGAATGCTCCCA7220.1450862580003416No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG7220.1450862580003416No Hit
GCCCTATACTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGC7200.14468435700865093No Hit
ATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCT7170.14408150552111487No Hit
GGTTTAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA7070.1420720005626614No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG7010.14086629758758928No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6990.1404643965958986No Hit
CTGTTTCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATAC6940.13945964411667186No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA6890.13845489163744512No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC6800.13664633717483698No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA6760.1358425351914556No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6710.13483778271222885No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAGGCTAAACAG6690.13443588172053814No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6630.13323017874546605No Hit
GGAAATGTGAAACCACAATAGTTGTCTGAGTTTTAGGCCCATATTAACGT6550.13162257477870326No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG6550.13162257477870326No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6520.13101972329116723No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT6500.13061782229947652No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6450.12961306982024978No Hit
ATCAAGGTATATTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACAACC6420.12901021833271373No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC6380.12820641634933233No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC6380.12820641634933233No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT6360.12780451535764165No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT6320.12700071337426025No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAATTCCAGATTGGGACCT6310.1267997628784149No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA6310.1267997628784149No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA6300.12659881238256956No Hit
GAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA6270.1259959608950335No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGGCACTCAGGCA6250.12559405990334283No Hit
CCCTTACAGTGAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG6240.12539310940749746No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATTCCTATGGGAG6230.1251921589116521No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG6210.12479025791996143No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6170.12398645593658002No Hit
TTGTACACCTCACCATACGGCACTCAGGCAAGCTATTCTGTGTTGGGGTG6130.12318265395319862No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT6040.12137409949059048No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG6020.12097219849889979No Hit
GTTTCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGG6020.12097219849889979No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5950.11956554502798235No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5940.11936459453213702No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC5940.11936459453213702No Hit
GTACAGGGCCCTGACTCTGGGATCTTGAAGAGTTTGGTGGAAAGTGGTGG5930.11916364403629165No Hit
GTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT5930.11916364403629165No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5900.11856079254875561No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTGGTA5850.11755604006952888No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5820.11695318858199281No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5820.11695318858199281No Hit
CCCCATGGGGGACTGTTGGGGTGGAGCCCTCAGGCTCAGGGCCTACTCAC5810.11675223808614747No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA5790.11635033709445677No Hit
GTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC5770.11594843610276608No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT5720.11494368362353934No Hit
GTACAGGGTCCCCAGTCTTCGATAAGGTTGACGATATGGCAGAGACAGTA5690.11434083213600331No Hit
CACCTTTCCTGTCTTTCTTTTGGGAGAGAAACTGTTCTTGAATATTTGGT5660.11373798064846724No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5620.11293417866508584No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA5510.11072372321078704No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG5500.11052277271494167No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA5440.10931706973986957No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5440.10931706973986957No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5390.10831231726064283No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5380.10811136676479749No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG5380.10811136676479749No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC5360.10770946577310679No Hit
GTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAG5230.10509710932711726No Hit
GTATATTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACAACCAGCACC5200.10449425783958122No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG5190.10429330734373586No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAGGCTAAAC5190.10429330734373586No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG5170.10389140635204516No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC5160.10369045585619982No Hit
GCTTTAATGCCTTTATATGCATGTATACAGGCTAAACAGGCTTTTACTTT5160.10369045585619982No Hit
CTTCTGGACTATCAAGGTATATTGCCCGTTTGTCCTCTAATTCCAGGATC5150.10348950536035449No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5120.10288665387281842No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA5070.10188190139359168No Hit
CCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGAAAC5050.10148000040190099No Hit
TCTATCAACAATGTCCTCCCGTAAATGAATGTCAGGAAAAAAAGGAGTTT5040.10127904990605566No Hit
AGATAAGGGAGTAGGCTGCCTTCCTGACTGCCGATTGGTGGAGGCAGGAG5020.10087714891436494No Hit
CCTTATGTGTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCT5020.10087714891436494No Hit
GTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAAAT5000.10047524792267425No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)