FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269110
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12240.45483259633607076No Hit
CCCGCGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC10420.38720225929917135No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9730.3615621864665007No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9720.3611905912080562No Hit
GGGTCCTAGGAATCCTGATGTTGTGCTCTCCATGTTCGGTGCAGGGTCCC8870.3296049942402735No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG8720.32403106536360593No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG8650.32142989855449444No Hit
ACACTTGGGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA7900.2935602541711568No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7560.2809260153840437No Hit
GTAACACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGCGA6850.25454275203448407No Hit
CTTATATAGAATGCCCGCCTTCCACAAAGTATGTAAATAATGCCTAGTTT6750.25082679945003905No Hit
GGTTTTAGGAATCCTGATGTTGTGCTCTCCATGTTCGGTGCAGGGTCCCC6560.24376648953959348No Hit
GTAAAACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGCGA6520.24228010850581547No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT6290.23373341756159194No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA6070.22555832187581287No Hit
ATTTACATACTTTGTGGAAGGCGGGCATTCTATATAAGAGAGAAACTACA5440.20214782059380923No Hit
GGTCCTAGGAATCCTGATGTTGTGCTCTCCATGTTCGGTGCAGGGTCCCC5390.2002898443015867No Hit
ATCCTAACCTTACCAAATATCTGCCCTTGGACAAAGGCATTAAACCATAT5380.1999182490431422No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5000.18579762922225113No Hit
GAACCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAAC4960.1843112481884731No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT4810.1787373193118056No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGCGAGTCCAA4790.17799412879491658No Hit
GTCAAGAAGACAGCCTACTCCCATCTCTCCACCTCTAAGAGACAGTCATC4740.17613615250269407No Hit
GCCTCTGGCCAGGGATCCTTGTTGGGGTTGAAGTCCCAATCTGGATTGTT4600.17093381888447104No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4430.1646166994909145No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4300.15978596113113597No Hit
GTAGAGTTTCCTTGAGCAGGACTCGTGCAGGTCTTGCATGGTCCCGTGCT4230.15718479432202445No Hit
CTGCACGGCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAG4160.15458362751291294No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG4020.1493812938946899No Hit
GGCCATACAGTGGAATTCCACAACATTCCACCAAGCTCTGCTAGACCCCA3980.14789491286091191No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG3850.14306417450113337No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT3700.13749024562446582No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG3640.13526067407379883No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG3630.13488907881535434No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC3570.1326595072646873No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC3540.1315447214893538No Hit
TGTATACCCAGAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTC3540.1315447214893538No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3520.13080153097246477No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGCG3500.1300583404555758No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT3470.12894355468024227No Hit
CTCTTATATAGAATGCCCGCCTTCCACAAAGTATGTAAATAATGCCTAGT3460.12857195942179778No Hit
TTTACATACTTTGTGGAAGGCGGGCATTCTATATAAGAGAGAAACTACAC3440.12782876890490877No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3390.12597079261268626No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTCACAGTTAATGAAAAA3370.12522760209579725No Hit
AGGTCTTACATAAGAGGACTCTTGGACTCGCAGCAATGTCAACGACCGAC3310.12299803054513024No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC3290.12225484002824125No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC3260.12114005425290773No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG3260.12114005425290773No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3150.11705250641001821No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCAAG3140.1166809111515737No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA3140.1166809111515737No Hit
ATGTAACACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC3070.1140797443424622No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGGCGATGGCC3070.1140797443424622No Hit
GTAAATAGACCTATTGATTGGAAAGTATGTCAGAGAATTGTGGGTCTTTT3070.1140797443424622No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA3050.11333655382557317No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTA3000.11147857753335066No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG3000.11147857753335066No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACCAAATGCC2990.11110698227490617No Hit
GTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGG2970.11036379175801718No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG2920.10850581546579464No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA2910.10813422020735015No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGTTTCACATTTCC2900.10776262494890565No Hit
CCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATATT2860.10627624391512765No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT2850.10590464865668314No Hit
TCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGCGAGAGGACAACAGAGT2830.10516145813979413No Hit
GAGTTCCGCAGTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGC2820.10478986288134963No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG2820.10478986288134963No Hit
CCTTTACCCCGTTGCCAGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG2800.10404667236446064No Hit
GTTGTCAATGTGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG2800.10404667236446064No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGATTTTTAGTACAATA2800.10404667236446064No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC2780.10330348184757161No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA2780.10330348184757161No Hit
GGGTTTACTGTTCCGGAATTGGAGCCACCAGCAGGAAAGTATAGGCCCCT2760.10256029133068262No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTT2760.10256029133068262No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAGATATTTGGTAAGGTT2750.10218869607223813No Hit
GTTCTATCCTAACCTTACCAAATATCTGCCCTTGGACAAAGGCATTAAAC2750.10218869607223813No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA2740.10181710081379361No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT2740.10181710081379361No Hit
GTCTATTAACAGGCAATTTTCGAAAACATTGCTTGATTTTTAGTACAATA2720.10107391029690461No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAATTTCAT2720.10107391029690461No Hit
ACAAAAATCCTCACAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCT2710.10070231503846011No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCAGGCAACGGTCAGGTC2700.10033071978001559No Hit
CCCAAGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC2700.10033071978001559No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)