FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354929
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCCAATGCCCCTATCTTATCAACACTTCCGGA27350.7705766505413759No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAACAAG17030.47981427271369764No Hit
ATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAG15660.44121500356409316No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA14330.40374272037506087No Hit
GTTCATGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAAAAAGTGA13600.38317522659461467No Hit
GTATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA13530.381203001163613No Hit
GTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACGTTGACAAACT12590.3547188310901617No Hit
AATACAAACAAGACCTTTGATCTAATCTCCTCCCCCAACTCCTCCCAGTC11440.3223179847237053No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC10780.30372271637426074No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA10360.2918893637882506No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG10060.28343696908395766No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTTTCGCCAACTTACAAGGCC9920.27949251822195426No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA9830.27695679981066634No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT9440.2659686866950855No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9200.2592067709316511No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG9080.25582581304993396No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8950.2521631086780737No Hit
GTGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATAC8870.24990913675692886No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA8780.24737341834564097No Hit
CTCTTTACACTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC8390.2363853052300601No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAAGAATTGGTGGGCGTTC8370.2358218122497739No Hit
CTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA8260.2327226008581998No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCGGTACG8070.22736941754548093No Hit
GAAACGATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGA7870.22173448774261895No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7760.21863527635104485No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTAT7620.21469082548904148No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC7500.2113098676073243No Hit
GTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAATTTT7210.20313921939317442No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG7180.20229397992274512No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATGATTAACTA7100.20004000800160032No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACACTTCAT7050.19863127555088483No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAAGAAT7040.1983495290607417No Hit
CTCAATTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCAC6820.19215110627759355No Hit
CATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTACGCGGG6620.18651617647473157No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAAGAGAGGTGC6540.18426220455358677No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA6520.1836987115733006No Hit
CTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGAACACA6490.18285347210287128No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA6390.18003600720144028No Hit
GGATAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAAT6370.1794725142211541No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT6340.1786272747507248No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6170.1738375844182921No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC6100.1718653589872904No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5700.16059549938156645No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA5650.15918676693085096No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGGAAAATTCCTATGGGAG5640.15890502044070787No Hit
TACATAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGCCTATT5640.15890502044070787No Hit
GGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGG5550.15636930202941995No Hit
GTTATGCCCCTATCTTATCAACACTTCCGGAGACTACTGTTGTTAGACGA5400.15214310467727346No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5380.15157961169698728No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAA5270.1484804003054132No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC5250.147916907325127No Hit
AGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAATTT5230.14735341434484078No Hit
GTACGACATCTTCTAGATACCGCCTCAGCTCTATATCGGGAAGCCTTAGA5220.1470716678546977No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG5190.1462264283842684No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGC5160.1453811889138391No Hit
TAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACG5080.14312721699269432No Hit
GATACTGGATCTTCCAAATTACCACCCACCCAGGTGGCTAGAGTCATTAG4970.14002800560112022No Hit
GGGTTACTCTTTACACTTCATGGGCTATGTCATTGGATGTTATGGGTCAT4910.13833752666026164No Hit
ATACAAACAAGACCTTTGATCTAATCTCCTCCCCCAACTCCTCCCAGTCC4890.13777403367997543No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT4890.13777403367997543No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4800.13523831526868754No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATACAATCTAAGC4790.13495656877854442No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG4770.13439307579825824No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4720.13298434334754275No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4630.13044862493625486No Hit
CTCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCAGTATGG4580.12903989248553935No Hit
GTTACAATGCCCCTATCTTATCAACACTTCCGGAGACTACTGTTGTTAGA4560.12847639950525316No Hit
TTTCCGGCTCCTCTGCCGATCCATACTGCGGAACTCCTAGCCGCTTGTTT4490.1265041740742515No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGAGC4470.12594068109396528No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC4470.12594068109396528No Hit
GGCCTATTGATTGGAAAGTTTGTCAACGTATTGTGGGTCTTTTGGGTTTT4420.12453194864324978No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4420.12453194864324978No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4400.12396845566296358No Hit
TGGTTACAGCGGTAAAAAGGGACTCAAGATGCTGTACAGACTTGGCCCCC4400.12396845566296358No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC4370.12312321619253427No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4370.12312321619253427No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCA4360.12284146970239118No Hit
CCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAAGTATGCCTCA4350.12255972321224809No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4330.12199623023196188No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA4260.12002400480096019No Hit
TGTTCGCTCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCA4250.1197422583108171No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG4230.11917876533053089No Hit
GCTTAGATTGTATACATGCATACAAGGGCATTAAAGCAGGATAACCACAT4220.1188970188403878No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT4200.1183335258601016No Hit
GTATGCATGTATACAATCTAAGCAGGCTTTCACTTTTTCGCCAACTTACA4190.1180517793699585No Hit
ATATTGGATCTTCCAAATTACCACCCACCCAGGTGGCTAGAGTCATTAGT4190.1180517793699585No Hit
TTTTCTGTATGATGTGTTCTTGTGGCAATGACCCATAACATCCAATGACA4160.1172065398995292No Hit
GTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCAAGCAATTC4140.11664304691924302No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG4140.11664304691924302No Hit
AGCCAGAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGC4130.1163613004290999No Hit
ACACATAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAAGAGCT4090.11523431446852751No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAAAAAGTGAAAGCCTGCT4090.11523431446852751No Hit
ATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCCCTCCT4060.11438907499809821No Hit
CTGTAACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA4050.11410732850795512No Hit
GTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAAAAAGTGA4020.11326208903752581No Hit
GTTATAATGCCCCTATCTTATCAACACTTCCGGAGACTACTGTTGTTAGA4000.11269859605723961No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAG3970.11185335658681031No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG3970.11185335658681031No Hit
ATGCAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC3950.11128986360652413No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTACGCGGGACGTCCTTTGTTTA3880.10931763817552242No Hit
CAATTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG3790.10678191976423454No Hit
ATACATGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAG3780.10650017327409145No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT3780.10650017327409145No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG3730.10509144082337596No Hit
CTTCTAGATACCGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGA3720.10480969433323284No Hit
GTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGT3720.10480969433323284No Hit
GACATAGCCCATGAAGTGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAG3710.10452794784308975No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT3680.10368270837266046No Hit
ACGACATCTTCTAGATACCGCCTCAGCTCTATATCGGGAAGCCTTAGAGT3670.10340096188251734No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG3650.10283746890223115No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT3600.10142873645151565No Hit
TGGTAACAGCGGTAAAAAGGGACTCAAGATGCTGTACAGACTTGGCCCCC3570.10058349698108635No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC3570.10058349698108635No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA3560.10030175049094325No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)