FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354929
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCCAATGCCCCTATCTTATCAACACTTCCGGA26730.7531083681525037No Hit
ATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAG16440.46319122979525484No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA15700.4423419895246655No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAACAAG13980.39388159322005245No Hit
GTTCATGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAAAAAGTGA13120.3696513950677459No Hit
GTATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA12590.3547188310901617No Hit
GTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACGTTGACAAACT12230.34457595744501013No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC10410.2932980962389661No Hit
CTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA10240.2885084059065334No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG9800.2761115603402371No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTTTCGCCAACTTACAAGGCC9580.26991313755708884No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA9490.267377419145801No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG9400.26484170073451313No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC9210.2594885174217942No Hit
CTCTTTACACTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC9160.2580797849710787No Hit
CATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTACGCGGG8830.24878215079635646No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTAT8730.24596468589492548No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8480.238921023641348No Hit
GTGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATAC8320.2344130797990584No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA8130.22905989648633954No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCGGTACG7640.21525431846932766No Hit
GAAACGATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGA7430.2093376421763226No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA7360.2073654167453209No Hit
AATACAAACAAGACCTTTGATCTAATCTCCTCCCCCAACTCCTCCCAGTC7300.2056749378044623No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT7210.20313921939317442No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC7190.2025757264128882No Hit
GGATAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAAT7160.20173048694245893No Hit
CTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGAACACA6970.19637730362974No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6920.19496857117902452No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTACGCGGGACGTCCTTTGTTTA6830.19243285276773664No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAAGAATTGGTGGGCGTTC6740.18989713435644875No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACACTTCAT6670.18792490892544705No Hit
GTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAATTTT6540.18426220455358677No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6490.18285347210287128No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT6400.18031775369158337No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC6370.1794725142211541No Hit
CTCAATTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCAC6360.17919076773101097No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG6240.1758098098492938No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA6160.173555837928149No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5980.16848440110557322No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGGAAAATTCCTATGGGAG5950.16763916163514392No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA5900.16623042918442843No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATGATTAACTA5680.16003200640128026No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG5570.15693279500970617No Hit
TTTCCGGCTCCTCTGCCGATCCATACTGCGGAACTCCTAGCCGCTTGTTT5460.1538335836181321No Hit
TACATAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGCCTATT5420.15270659765755967No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5400.15214310467727346No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC5350.15073437222655797No Hit
GGGTTACTCTTTACACTTCATGGGCTATGTCATTGGATGTTATGGGTCAT5230.14735341434484078No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAAGAGAGGTGC5210.1467899213645546No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5150.14509944242369602No Hit
CTCTTTACGCGGCCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5120.1442542029532667No Hit
CTCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCAGTATGG5120.1442542029532667No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAAGAAT5070.14284547050255123No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5070.14284547050255123No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG4950.13946451262083404No Hit
GTTATGCCCCTATCTTATCAACACTTCCGGAGACTACTGTTGTTAGACGA4950.13946451262083404No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4880.13749228718983234No Hit
GGCCTATTGATTGGAAAGTTTGTCAACGTATTGTGGGTCTTTTGGGTTTT4810.13552006175883063No Hit
TAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACG4800.13523831526868754No Hit
GTATGCATGTATACAATCTAAGCAGGCTTTCACTTTTTCGCCAACTTACA4800.13523831526868754No Hit
GTACGACATCTTCTAGATACCGCCTCAGCTCTATATCGGGAAGCCTTAGA4750.13382958281797205No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC4740.13354783632782893No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA4710.13270259685739966No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT4690.13213910387711345No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG4650.13101211791654105No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG4590.12932163897568244No Hit
GCTTAGATTGTATACATGCATACAAGGGCATTAAAGCAGGATAACCACAT4540.12791290652496698No Hit
TCTCGGGGCCGCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCG4510.12706766705453768No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG4490.1265041740742515No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA4480.12622242758410837No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCTTCTCGGGGCCG4470.12594068109396528No Hit
AGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAATTT4410.12425020215310668No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCA4390.12368670917282049No Hit
GATACTGGATCTTCCAAATTACCACCCACCCAGGTGGCTAGAGTCATTAG4380.12340496268267737No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGAGC4370.12312321619253427No Hit
GTCCCGTGCTGGTGGTTGAAGATCATTGAGATTCCCGAGATTGAGATCTT4350.12255972321224809No Hit
ATACATGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAG4340.122277976722105No Hit
CTGTAACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA4340.122277976722105No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT4190.1180517793699585No Hit
CTGCCTCGTCGTCTAACAACAGTAGTCTCCGGAAGTGTTGATAAGATAGG4180.11777003287981538No Hit
GTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCAAGCAATTC4070.1146708214882413No Hit
GGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGG4070.1146708214882413No Hit
CCCATGAAGTGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAA4060.11438907499809821No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC4060.11438907499809821No Hit
CTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC4050.11410732850795512No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4050.11410732850795512No Hit
TGGTTACAGCGGTAAAAAGGGACTCAAGATGCTGTACAGACTTGGCCCCC4040.11382558201781202No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATACAATCTAAGC3990.11241684956709652No Hit
ATATTGGATCTTCCAAATTACCACCCACCCAGGTGGCTAGAGTCATTAGT3970.11185335658681031No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG3930.11072637062623791No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3910.11016287764595173No Hit
ATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCCCTCCT3900.10988113115580862No Hit
ATCTAAGCAGGCTTTCACTTTTTCGCCAACTTACAAGGCCTTTCTGTGTA3880.10931763817552242No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGC3870.10903589168537932No Hit
TGTTCGCTCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCA3860.10875414519523623No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3840.10819065221495004No Hit
GTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGT3830.10790890572480695No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT3820.10762715923466383No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG3810.10734541274452074No Hit
CCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAAGTATGCCTCA3800.10706366625437763No Hit
GTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAAAAAGTGA3760.10593668029380524No Hit
CAATTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG3740.10537318731351905No Hit
GATTGTATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGT3730.10509144082337596No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAAAAAGTGAAAGCCTGCT3710.10452794784308975No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAA3690.10396445486280355No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT3660.10311921539237424No Hit
GACATAGCCCATGAAGTGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAG3650.10283746890223115No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT3640.10255572241208806No Hit
CTTCTAGATACCGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGA3610.10171048294165876No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCT3600.10142873645151565No Hit
TTTTCTGTATGATGTGTTCTTGTGGCAATGACCCATAACATCCAATGACA3570.10058349698108635No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)