FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302420
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA19580.6474439521195688No Hit
GCACAATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA16120.5330335295284703No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC16060.5310495337609946No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG14810.489716288605251No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCGGAGAGTCCAA14680.4854176311090536No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA8840.2923087097414192No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC8240.27246875206666227No Hit
CCTTTAAATGAATGTGAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG8020.2651941009192514No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA7640.25262879439190533No Hit
GTATGTAAATAGTGCCTAGTTTTGAAGTAATGATTAACTGCATTTTCAGG7250.23973282190331324No Hit
GACACATCCAGCGATAACCAGGACAAATTGGAGGACAAGAGGTTGGTGAG7220.23874082401957541No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT7090.2344421665233781No Hit
CATGTATACAATCTAAGCGGGCTTTCACTTTCTCGCCAACTTACAAGGCT6920.22882084518219695No Hit
GGATATATCTGCGGCGTTTTATCATATTCCTCTTCATCCTGCTGCTATGC6740.22286885787976984No Hit
GTATCCTTGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCC6680.22088486211229416No Hit
GGTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT6190.20468223001124264No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6070.20071423847629125No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC6050.20005290655379934No Hit
CTTTAGGGCATGGACATTGACCCATATAAAGAATTTGGAGCTTCTGTGGA6030.19939157463130744No Hit
ATATCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA5970.19740757886383176No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAATTTCAT5590.1848422723364857No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAGTGCCTAGTTT5560.18385027445274785No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5560.18385027445274785No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC5440.17988228291779645No Hit
CCTCAGCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCT5430.1795516169565505No Hit
CCCTTAATTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG5340.17657562330533694No Hit
CATTTACAGGAAGACATTATTAATAGATGTCAACAATATGTAGGCCCTCT5330.17624495734409099No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5280.17459162753786125No Hit
ATCCTAACCTTACCAAATATTTGCCTTTGGACAAAGGCATTAAACCGTAT5270.1742609615766153No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5260.17393029561536935No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5200.17194629984789367No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCCGGCA5090.1683089742741882No Hit
GCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA4940.16334898485549898No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4930.16301831889425303No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4920.16268765293300708No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4900.16202632101051517No Hit
AAGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT4800.15871966139805568No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGCGGGTCACCATA4780.15805832947556378No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT4730.15640499966933402No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4690.15508233582435024No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT4660.1540903379406124No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAGTCTCCTTTTTTCA4590.15177567621189075No Hit
GCACGAGTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA4540.150122346405661No Hit
GCTTTGGGGCATGGACATTGACCCATATAAAGAATTTGGAGCTTCTGTGG4530.14979168044441507No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4530.14979168044441507No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA4510.14913034852192317No Hit
TCCACAGAGTATGTAAATAGTGCCTAGTTTTGAAGTAATGATTAACTGCA4500.14879968256067722No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4350.14383969314198797No Hit
CCTAACGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4330.14317836121949606No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4310.14251702929700416No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC4200.13887970372329872No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4200.13887970372329872No Hit
CTTTGGGGCATGGACATTGACCCATATAAAGAATTTGGAGCTTCTGTGGA4190.13854903776205277No Hit
GTCCACAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4180.13821837180080682No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4120.13623437603333113No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA4100.13557304411083923No Hit
GGGTAAAGGTTCAAATATTGTTTACACAGAAAAGCCTTGTAAGTTGGCGA4060.13425038026585542No Hit
GGAAAATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT4060.13425038026585542No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4050.13391971430460947No Hit
TCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4030.13325838238211757No Hit
CTCTTACAGTTAATGAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGG4030.13325838238211757No Hit
CAATCCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTT4010.1325970504596257No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC3930.12995172276965808No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3830.1266450631571986No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG3800.12565306527346076No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGCGGGTCACCATATT3790.1253223993122148No Hit
GGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAG3780.12499173335096886No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC3780.12499173335096886No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC3760.12433040142847696No Hit
GCATTTTCAGGATAATACGGTTTAATGCCTTTGTCCAAAGGCAAATATTT3740.12366906950598505No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA3700.12234640566100126No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC3690.12201573969975531No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC3620.11970107797103366No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC3580.11837841412604988No Hit
GCTTTAGGGCATGGACATTGACCCATATAAAGAATTTGGAGCTTCTGTGG3550.11738641624231202No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG3540.11705575028106607No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCATTG3520.11639441835857417No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA3510.11606375239732822No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA3490.11540242047483633No Hit
CCATACAGCACTCCGGCAAGCTATTCTTTGTTGGGGTGAGTTGATGAATC3460.11441042259109847No Hit
GGAATTGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG3450.11407975662985252No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGACTAAAATTAATTATG3440.11374909066860657No Hit
CTATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTA3420.11308775874611468No Hit
ATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG3400.11242642682362278No Hit
CAAGGACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACAAT3400.11242642682362278No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG3400.11242642682362278No Hit
CTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCA3360.11110376297863897No Hit
AATTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA3310.10945043317240923No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3300.10911976721116327No Hit
AAAGAATTGTGGGTCTTTTGGGCTTTGCTGCCCCTTTTACACAATGTGGC3290.10878910124991732No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC3280.10845843528867137No Hit
ATCTAAGCGGGCTTTCACTTTCTCGCCAACTTACAAGGCTTTTCTGTGTA3260.10779710336617948No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAATTTCATGGGATATG3240.10713577144368759No Hit
GGATGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC3230.10680510548244165No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATA3230.10680510548244165No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCCGCAATGTCAACGACCGACCT3220.1064744395211957No Hit
TCCTAGTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGT3200.1058131075987038No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC3190.10548244163745785No Hit
ATACAGCACTCCGGCAAGCTATTCTTTGTTGGGGTGAGTTGATGAATCTG3170.10482110971496594No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA3170.10482110971496594No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCT3110.10283711394749025No Hit
ATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG3070.10151445010250645No Hit
CCTTTATATGCATGTATACAATCTAAGCGGGCTTTCACTTTCTCGCCAAC3040.1005224522187686No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAGTGCCTAGT3040.1005224522187686No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG3030.10019178625752265No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)