FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302420
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA19110.6319026519410093No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC14250.47119899477547783No Hit
GCACAATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA13520.4470603796045235No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCGGAGAGTCCAA11760.38886317042523644No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG11350.3753058660141525No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7780.2572581178493486No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA7130.2357648303683619No Hit
CCTTTAAATGAATGTGAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG6660.22022353018980226No Hit
GTATGTAAATAGTGCCTAGTTTTGAAGTAATGATTAACTGCATTTTCAGG6420.2122875471198995No Hit
ATATCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA6130.20269823424376696No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT6020.1990609086700615No Hit
CATGTATACAATCTAAGCGGGCTTTCACTTTCTCGCCAACTTACAAGGCT5910.19542358309635605No Hit
GACACATCCAGCGATAACCAGGACAAATTGGAGGACAAGAGGTTGGTGAG5650.18682626810396136No Hit
GTATCCTTGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCC5640.1864956021427154No Hit
GGATATATCTGCGGCGTTTTATCATATTCCTCTTCATCCTGCTGCTATGC5590.1848422723364857No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5520.18252761060776404No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT5440.17988228291779645No Hit
CATTTACAGGAAGACATTATTAATAGATGTCAACAATATGTAGGCCCTCT5420.17922095099530455No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC5280.17459162753786125No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5230.17293829773163152No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAGTGCCTAGTTT5200.17194629984789367No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5200.17194629984789367No Hit
CTTTAGGGCATGGACATTGACCCATATAAAGAATTTGGAGCTTCTGTGGA5140.16996230408041796No Hit
CCTCAGCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCT5090.1683089742741882No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5090.1683089742741882No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA5050.16698631042920442No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAATTTCAT4980.16467164870048276No Hit
GGTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT4810.15905032735930164No Hit
CCCTTAATTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG4800.15871966139805568No Hit
ATCCTAACCTTACCAAATATTTGCCTTTGGACAAAGGCATTAAACCGTAT4690.15508233582435024No Hit
AAGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT4690.15508233582435024No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCCGGCA4630.15309834005687453No Hit
GCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA4580.1514450102506448No Hit
GCTTTGGGGCATGGACATTGACCCATATAAAGAATTTGGAGCTTCTGTGG4560.1507836783281529No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4440.1468156867932015No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC4430.14648502083195555No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC4360.14417035910323392No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4320.1428476952582501No Hit
GCACGAGTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA4320.1428476952582501No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4290.14185569737451226No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4180.13821837180080682No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGCGGGTCACCATA4140.13689570795582304No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4130.13656504199457709No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4020.13292771642087164No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA3990.1319357185371338No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAGTCTCCTTTTTTCA3950.13061305469214998No Hit
TCCACAGAGTATGTAAATAGTGCCTAGTTTTGAAGTAATGATTAACTGCA3910.1292903908471662No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3900.12895972488592025No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA3900.12895972488592025No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG3890.1286290589246743No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA3880.12829839296342835No Hit
CCTAACGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3820.12631439719595267No Hit
GGGTAAAGGTTCAAATATTGTTTACACAGAAAAGCCTTGTAAGTTGGCGA3810.12598373123470671No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG3790.1253223993122148No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT3780.12499173335096886No Hit
CTTTGGGGCATGGACATTGACCCATATAAAGAATTTGGAGCTTCTGTGGA3750.123999735467231No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGCGGGTCACCATATT3720.12300773758349315No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC3720.12300773758349315No Hit
GGATGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC3680.12168507373850936No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA3620.11970107797103366No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATA3610.11937041200978771No Hit
CTCTTACAGTTAATGAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGG3590.1187090800872958No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3580.11837841412604988No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT3570.11804774816480393No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC3560.11771708220355798No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA3490.11540242047483633No Hit
AATTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA3470.11474108855234443No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3470.11474108855234443No Hit
GGAAAATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT3460.11441042259109847No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC3450.11407975662985252No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCCGCAATGTCAACGACCGACCT3420.11308775874611468No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA3420.11308775874611468No Hit
ATCTAAGCGGGCTTTCACTTTCTCGCCAACTTACAAGGCTTTTCTGTGTA3390.11209576086237683No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC3360.11110376297863897No Hit
GCATTTTCAGGATAATACGGTTTAATGCCTTTGTCCAAAGGCAAATATTT3340.11044243105614708No Hit
ATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG3330.11011176509490113No Hit
CGGTGGTTTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG3290.10878910124991732No Hit
CCATACAGCACTCCGGCAAGCTATTCTTTGTTGGGGTGAGTTGATGAATC3210.10614377355994975No Hit
TCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT3200.1058131075987038No Hit
CAATCCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTT3190.10548244163745785No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC3130.10349844586998215No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA3110.10283711394749025No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGACTAAAATTAATTATG3090.10217578202499834No Hit
ATACAGCACTCCGGCAAGCTATTCTTTGTTGGGGTGAGTTGATGAATCTG3060.1011837841412605No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3050.10085311818001455No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)