FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379440
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA20890.5505481762597513No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG19280.5081172253847775No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC18540.48861480075901326No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC18350.4836074214632089No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG15490.40823318574741724No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA14180.3737086232342399No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14050.3702825216107949No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA13300.350516550706304No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT12770.3365485979337972No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG12430.3275880244570947No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT12340.32521610794855577No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT12050.317573265865486No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT10560.2783048703352308No Hit
GTATAAAGGGACTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC9760.2572211680371073No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA9400.24773350200295174No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT9130.240617752477335No Hit
ATATGATCTTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA8820.23244781783681215No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC8060.21241830065359477No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT7990.21057347670250895No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC7830.20635673624288425No Hit
TCCTCAGGCCATGCAGTGGAATTCCACTGCCTTCCACCAAGCTCTGCAGG7680.2024035420619861No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT7350.19370651486401014No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7260.19133459835547123No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC7200.18975332068311196No Hit
GTCTAAGGGCAGATACTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA7180.18922622812565887No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT7040.18553658022348724No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG7010.1847459413873076No Hit
TACTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT6990.18421884882985454No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGGTG6940.1829011174362218No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6730.17736664558296436No Hit
CAGTAAACCCTGCTCCGAATATTGCCTCTCACATCTCGTCAATCTCCGCG6670.17578536791060512No Hit
CTCTTTGGAAGGCTGGTATTCTATATAAGAGGGAAACCACACGTAGCGCA6650.175258275353152No Hit
GTACTGTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA6640.17499472907442545No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6540.17235926628716003No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAATACAGACCCCT6460.17025089605734767No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGCTCCGA6440.16972380349989458No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA6440.16972380349989458No Hit
CACTAAGTATCTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC6420.16919671094244151No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT6420.16919671094244151No Hit
GTATCAAGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTG6400.1686696183849884No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT6350.1673518869913557No Hit
CTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT6330.16682479443390258No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC6230.16418933164663715No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG6210.16366223908918404No Hit
CTTCTAGACACCGCCTCAGCTCTGTATCAAGAAGCCTTAGAGTCTCCTGA6150.1620809614168248No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATATTCAAGAA6120.16129032258064516No Hit
GCCTAATCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGC6110.1610267763019186No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6030.15891840607210628No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCT5920.15601939700611428No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5880.1549652118912081No Hit
GTACAAAACCTACGGATGGAAATTGCACCTGTATTCCCATCCCATCGTCC5840.15391102677630192No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA5810.15312038794012228No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG5640.148640101201771No Hit
ATTGACCACAGTGTCAACAATTCCTCCTCCTGCCTCCACCAATCGGCAGT5600.14758591608686486No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA5510.14521399957832595No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAGATACTTAGTGTGGGT5480.14442336074214632No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT5460.14389626818469323No Hit
GTGGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAATACCGCCGTG5400.14231499051233396No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT5280.1391524351676154No Hit
ACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGGAAATTGC5270.1388888888888889No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT5260.13862534261016235No Hit
ATGTATATTTCCGCGAGAGGACGACAGAATTGTCAGTTCCGATGAGCTTT5140.1354627872654438No Hit
CAATAGGCCTGTTAACAGGAAGTTTACTAAAACAGTGTTTGATCTTTTGT5130.13519924098671726No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5060.13335441703563147No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG5050.13309087075690493No Hit
GGATTAAAGATAGGTACTGTAGAGGAATAAAGCCCAGTAAAGTTTCCCAC5000.13177313936327217No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA5000.13177313936327217No Hit
GTATATTTCCGCGAGAGGACGACAGAATTGTCAGTTCCGATGAGCTTTGC4970.13098250052709257No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4970.13098250052709257No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA4960.130718954248366No Hit
GTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC4880.12861058401855366No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG4880.12861058401855366No Hit
GTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT4840.12755639890364748No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT4840.12755639890364748No Hit
GTGTTTGATCTTTTGTACAATATGATCTTGTGGCAATGTTCCCCAACTTC4830.12729285262492093No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4800.1265022137887413No Hit
AGATCATATTGTACAAAAGATCAAACACTGTTTTAGTAAACTTCCTGTTA4730.1246573898376555No Hit
ATTCTATCCTACCCACACTAAGTATCTGCCCTTAGACAAAGGAATTAAAC4710.12413029728020239No Hit
CTCCGAAAGAGACCAAATATTCAAGAACAGTCTCTCTTCCAAAAGTAAGG4660.12281256588656969No Hit
CAGTTGGACCCTGCATTCGGAGCCAACTCAAACAATCCAGATTGGGACTT4660.12281256588656969No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCATGTACTGTTTACTT4650.12254901960784313No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4650.12254901960784313No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC4650.12254901960784313No Hit
GGTTTAAAGATCAGGCAACTATTGTGGTTTCATATATCTTGCCTTACTTT4640.12228547332911659No Hit
GGAAAATACAGACCCCTGACTCTGGGATCCTGCAGAGCTTGGTGGAAGGC4640.12228547332911659No Hit
CATATATCTTGCCTTACTTTTGGAAGAGAGACTGTTCTTGAATATTTGGT4600.1212312882142104No Hit
GTCCTGGGCTTTCGCAAAATACCTATGGGAGTGGGCCTCAGTCCGTTTCT4600.1212312882142104No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA4590.12096774193548387No Hit
CTATTGTGGTTTCATATATCTTGCCTTACTTTTGGAAGAGAGACTGTTCT4540.11965001054185115No Hit
TCCTAATACAAAGACCTTTAACCTAATCTCCTCCCCCAGCTCCTCCCAGT4530.11938646426312459No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG4530.11938646426312459No Hit
GTCCAACTGATGATCGGGAAAGAATCCCAGAGGGTTGGGAACAGAAAGAT4520.11912291798439807No Hit
TGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTATAAAGGGACTC4500.11859582542694497No Hit
ACATAGAGTTGCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCCGTA4480.11806873286949189No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAAC4460.11754164031203879No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG4430.11675100147585915No Hit
CCTGTATTCCCATCCCATCGTCCTGGGCTTTCGCAAAATACCTATGGGAG4380.11543327008222645No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCGTGAGTCCCTTTATACC4350.11464263124604679No Hit
GTTTAAATGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTATAAA4270.11253426101623445No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG4260.11227071473750791No Hit
GCCTTCCACCAAGCTCTGCAGGATCCCAGAGTCAGGGGTCTGTATTTTCC4180.11016234450769555No Hit
CTCCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGG4170.109898798228969No Hit
ACTGTGCAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG4150.10937170567151591No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4140.10910815939278937No Hit
ATTTTGGGGTGGAGCCCTCAGGCTCAGGGCATATTGACCACAGTGTCAAC4140.10910815939278937No Hit
CCTTTAACCTAATCTCCTCCCCCAGCTCCTCCCAGTCCTTAAACACACAG4130.10884461311406284No Hit
GCTCATCGGAACTGACAATTCTGTCGTCCTCTCGCGGAAATATACATCGT4130.10884461311406284No Hit
GAATACAGGTGCAATTTCCATCCGTAGGTTTTGTACAGCAACATGAGGGA4120.10858106683533629No Hit
GCATGGGAGGTTGGTCATCAAAACCTCGCAAAGGCATGGGGACGAATCTT4040.10647269660552393No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT4040.10647269660552393No Hit
CCCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC4030.10620915032679738No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCCTCTACAG4020.10594560404807085No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGGGAC4020.10594560404807085No Hit
TTCCCCCACTGTTTGGCTTTCAGCTATATGGATGATGTGGTATTGGGGGC3970.10462787265443811No Hit
ATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTATTGGTTCT3970.10462787265443811No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAG3960.10436432637571157No Hit
GGTCCTAGGAATCCTGATGTGATGTTCTCCATGTTCGTCACAGGGTCCCC3940.10383723381825849No Hit
GGCCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGCTTT3920.10331014126080539No Hit
CTCCTACCTGGTTGGCTGCTGGCCAGTGGTCCTTGATGGGGTTGAAGTCC3910.10304659498207885No Hit
CTTTAAACCCATGTTAGTATTAACATAATTGACTACTAGATCCCTGGATG3860.10172886358844613No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCATCAAAAC3850.10146531730971958No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAGGATCTGATGGGCGTTC3840.10120177103099305No Hit
CCTCTAAGAGACAGTCATCCTCAGGCCATGCAGTGGAATTCCACTGCCTT3830.10093822475226649No Hit
GTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG3830.10093822475226649No Hit
ATCCCAGAGTCAGGGGTCTGTATTTTCCTGCTGGTGGCTCCAGTTCAGGA3810.10041113219481342No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATATTCCTCTTCATCCT3800.10014758591608687No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)