FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379440
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA20950.5521294539321104No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG18660.49177735610373186No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG16300.42958043432426735No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC16070.4235188699135568No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC14170.3734450769555134No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13590.3581593927893738No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA13120.34577271768922624No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA13070.3444549862955935No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA12620.33259540375289903No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG12280.3236348302761965No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT12010.3165190807505798No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT11720.30887623866751No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT11570.30492304448661184No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT10130.26697238034998944No Hit
GTATAAAGGGACTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC9690.2553763440860215No Hit
ATATGATCTTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA9250.24378030782205357No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT9240.24351676154332702No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC8540.2250685220324689No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT8130.21426312460468055No Hit
TCCTCAGGCCATGCAGTGGAATTCCACTGCCTTCCACCAAGCTCTGCAGG7470.19686907020872868No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC7370.1942336074214632No Hit
GTCTAAGGGCAGATACTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA7250.19107105207674466No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC7140.1881720430107527No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG7110.18738140417457305No Hit
TACTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT7060.18606367278094033No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT7020.18500948766603414No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAATACAGACCCCT6870.181056293485136No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC6750.17789373814041745No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGCTCCGA6730.17736664558296436No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGGTG6670.17578536791060512No Hit
CTCTTTGGAAGGCTGGTATTCTATATAAGAGGGAAACCACACGTAGCGCA6640.17499472907442545No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT6630.17473118279569894No Hit
CAGTAAACCCTGCTCCGAATATTGCCTCTCACATCTCGTCAATCTCCGCG6600.1739405439595193No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA6580.1734134514020662No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6580.1734134514020662No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6560.17288635884461312No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT6490.1710415348935273No Hit
GTATCAAGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTG6380.16814252582753533No Hit
GCCTAATCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGC6360.16761543327008221No Hit
CACTAAGTATCTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC6300.16603415559772297No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6190.16313514653173097No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG6150.1620809614168248No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA6130.1615538688593717No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6020.15865485979337973No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5930.15628294328484083No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATATTCAAGAA5860.154438119333755No Hit
CTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT5810.15312038794012228No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCT5790.1525932953826692No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG5730.15101201771030992No Hit
CTTCTAGACACCGCCTCAGCTCTGTATCAAGAAGCCTTAGAGTCTCCTGA5650.14890364748049756No Hit
GTACTGTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA5600.14758591608686486No Hit
GGATTAAAGATAGGTACTGTAGAGGAATAAAGCCCAGTAAAGTTTCCCAC5530.14574109213577904No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5470.14415981446341977No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG5460.14389626818469323No Hit
ACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGGAAATTGC5430.1431056293485136No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT5410.1425785367910605No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT5400.14231499051233396No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAGATACTTAGTGTGGGT5380.1417878979548809No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA5370.14152435167615435No Hit
GTACAAAACCTACGGATGGAAATTGCACCTGTATTCCCATCCCATCGTCC5310.13994307400379508No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5280.1391524351676154No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA5270.1388888888888889No Hit
GCCTTCCACCAAGCTCTGCAGGATCCCAGAGTCAGGGGTCTGTATTTTCC5180.13651697238035No Hit
GTGGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAATACCGCCGTG5180.13651697238035No Hit
AGATCATATTGTACAAAAGATCAAACACTGTTTTAGTAAACTTCCTGTTA5110.13467214842926417No Hit
CTCCGAAAGAGACCAAATATTCAAGAACAGTCTCTCTTCCAAAAGTAAGG5100.13440860215053765No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT5020.1323002319207253No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG4910.1294012228547333No Hit
GGTTTTGTACAGCAACATGAGGGAAACATAGAGTTGCCTTGAGCAGGAGT4900.12913767657600675No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC4840.12755639890364748No Hit
GTGTTTGATCTTTTGTACAATATGATCTTGTGGCAATGTTCCCCAACTTC4830.12729285262492093No Hit
GTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT4800.1265022137887413No Hit
CATATATCTTGCCTTACTTTTGGAAGAGAGACTGTTCTTGAATATTTGGT4800.1265022137887413No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4790.12623866751001475No Hit
GTATATTTCCGCGAGAGGACGACAGAATTGTCAGTTCCGATGAGCTTTGC4790.12623866751001475No Hit
CAATAGGCCTGTTAACAGGAAGTTTACTAAAACAGTGTTTGATCTTTTGT4770.1257115749525617No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4750.12518448239510857No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA4740.12492093611638205No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG4720.12439384355892895No Hit
ATTGACCACAGTGTCAACAATTCCTCCTCCTGCCTCCACCAATCGGCAGT4700.12386675100147587No Hit
ATGTATATTTCCGCGAGAGGACGACAGAATTGTCAGTTCCGATGAGCTTT4660.12281256588656969No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATATTCCTCTTCATCCT4650.12254901960784313No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4640.12228547332911659No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT4620.12175838077166351No Hit
GTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC4590.12096774193548387No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4580.12070419565675733No Hit
GTCCAACTGATGATCGGGAAAGAATCCCAGAGGGTTGGGAACAGAAAGAT4580.12070419565675733No Hit
CTATTGTGGTTTCATATATCTTGCCTTACTTTTGGAAGAGAGACTGTTCT4520.11912291798439807No Hit
ATTCTATCCTACCCACACTAAGTATCTGCCCTTAGACAAAGGAATTAAAC4500.11859582542694497No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4500.11859582542694497No Hit
GTTTAAATGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTATAAA4490.11833227914821844No Hit
GTTCTTGAATATTTGGTCTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4480.11806873286949189No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCATGTACTGTTTACTT4470.11780518659076533No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAAC4470.11780518659076533No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA4460.11754164031203879No Hit
GCATGGGAGGTTGGTCATCAAAACCTCGCAAAGGCATGGGGACGAATCTT4420.11648745519713263No Hit
CCTGTATTCCCATCCCATCGTCCTGGGCTTTCGCAAAATACCTATGGGAG4400.11596036263967953No Hit
GGAAAATACAGACCCCTGACTCTGGGATCCTGCAGAGCTTGGTGGAAGGC4380.11543327008222645No Hit
CAGTTGGACCCTGCATTCGGAGCCAACTCAAACAATCCAGATTGGGACTT4350.11464263124604679No Hit
GGTTTAAAGATCAGGCAACTATTGTGGTTTCATATATCTTGCCTTACTTT4310.11358844613114064No Hit
CTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCTTCCTGACTGCCGATTG4220.11121652962260173No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCGTGAGTCCCTTTATACC4210.11095298334387518No Hit
ACATAGAGTTGCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCCGTA4200.11068943706514865No Hit
GTCCTGGGCTTTCGCAAAATACCTATGGGAGTGGGCCTCAGTCCGTTTCT4200.11068943706514865No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG4180.11016234450769555No Hit
TGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTATAAAGGGACTC4100.10805397427788319No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGGGAC4090.10779042799915665No Hit
GGTCCTAGGAATCCTGATGTGATGTTCTCCATGTTCGTCACAGGGTCCCC4070.10726333544170355No Hit
GTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG4060.10699978916297703No Hit
GAATACAGGTGCAATTTCCATCCGTAGGTTTTGTACAGCAACATGAGGGA4050.10673624288425047No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT4030.10620915032679738No Hit
GAATTAAACCTTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACC4030.10620915032679738No Hit
TTCCCCCACTGTTTGGCTTTCAGCTATATGGATGATGTGGTATTGGGGGC4020.10594560404807085No Hit
CCCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC4020.10594560404807085No Hit
GCATGGCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGC3960.10436432637571157No Hit
ATACTGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTGATGACTCTA3960.10436432637571157No Hit
CCTCTAAGAGACAGTCATCCTCAGGCCATGCAGTGGAATTCCACTGCCTT3940.10383723381825849No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG3940.10383723381825849No Hit
CTCCTACCTGGTTGGCTGCTGGCCAGTGGTCCTTGATGGGGTTGAAGTCC3930.10357368753953194No Hit
ATTTTGGGGTGGAGCCCTCAGGCTCAGGGCATATTGACCACAGTGTCAAC3930.10357368753953194No Hit
TTCAAGAACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA3870.10199240986717267No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCATCAAAAC3870.10199240986717267No Hit
GTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCTTCCTGACTGCCGAT3870.10199240986717267No Hit
TCCTAATACAAAGACCTTTAACCTAATCTCCTCCCCCAGCTCCTCCCAGT3860.10172886358844613No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCTCGGGGCCG3840.10120177103099305No Hit
GTTTTGTACAGCAACATGAGGGAAACATAGAGTTGCCTTGAGCAGGAGTC3840.10120177103099305No Hit
CTCCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGG3830.10093822475226649No Hit
ATTTACATACTCTTTGGAAGGCTGGTATTCTATATAAGAGGGAAACCACA3830.10093822475226649No Hit
ATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTATTGGTTCT3820.10067467847353995No Hit
GCCCAGGACGATGGGATGGGAATACAGGTGCAATTTCCATCCGTAGGTTT3810.10041113219481342No Hit
GTTTTAGTAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA3800.10014758591608687No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)