FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437501
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA24160.5522273091947218No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCGAGTACAAAGACC16960.38765625678569876No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC15650.3577134680835015No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGACAGTCCAA15170.34674206458956663No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT11880.27154223647488807No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG11320.258742265731964No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT11290.2580565530135931No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10860.24822800405027648No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG10080.23039947337263228No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG9950.2274280515930249No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT9900.22628519706240668No Hit
ATTCTGAACAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT9120.20845666638476257No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTATATATCCCATGAA8880.2029709646377951No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC8820.20159953920105325No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA8180.1869710012091401No Hit
GTATCGGGAGGCCTTAGAGTCTCCAGAACATTGTTCACCTCATCATACGG8040.18377100852340908No Hit
GGATAATACGGTTTGATCCCTTTATCTAAAGGCAAATATTTAGTAACATT7790.178056735870318No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCTGTGGGCGTTC7750.17714245224582345No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC7620.17417103046621607No Hit
ATATAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA7610.17394245956009244No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG7600.17371388865396878No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7570.17302817593559786No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACTAAATATTCAAGAA7290.16662819056413586No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC7210.1647996233151467No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7170.16388533969065214No Hit
CTTTAGGGCATGGACATTGACGCGTATAAAGAATTTGGAGCTTCTGTGGA7150.16342819787840485No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA7000.1599996342865502No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAAGGCAAATA6720.15359964891508818No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT6620.15131393985385178No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG6600.15085679804160448No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6520.14902823079261532No Hit
AAAACTCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGA6450.14742823444974984No Hit
GTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC6370.14559966720076067No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG6350.14514252538851338No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA6350.14514252538851338No Hit
ATGTATCTGCGGCGTTTTATCATCTTCCTCTGCATCCTGCTGCTATGCCT6320.1444568126701425No Hit
TCTTATTGGTTCTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTA6300.1439996708578952No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6290.14377109995177154No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6270.14331395813952424No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6230.1423996745150297No Hit
CTCCAAAAGACACTAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA6160.14079967817216418No Hit
GATGTATACTTGCGGGAGAGTACGACAGAATTGTCAGTCCCGATGAGTTT6110.13965682364154597No Hit
CAGACACATCCAGCGATAACCAGGACAAATTGGAGGACAACAGGTTGGTG6070.13874254001705139No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT5940.13577111823744403No Hit
CAATTATATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG5930.1355425473313204No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5920.13531397642519674No Hit
CCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGAAGATGATAAAA5900.13485683461294945No Hit
GCCTTAGAGTCTCCAGAACATTGTTCACCTCATCATACGGCACTCAGGCA5900.13485683461294945No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA5830.13325683827008394No Hit
CCTAAAACTCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTG5820.1330282673639603No Hit
TAGCCGACTACTAATTCCCTGGATGCTGGATCTTCCAAATTACTTCCCAC5780.13211398373946573No Hit
GAAATGATGTATACTTGCGGGAGAGTACGACAGAATTGTCAGTCCCGATG5670.12959970377210567No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGTACGACAGAAT5670.12959970377210567No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC5630.1286854201476111No Hit
CCCCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG5560.1270854238047456No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACTAAATATTCAA5560.1270854238047456No Hit
ATTATATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTG5530.12639971108637466No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5490.1254854274618801No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC5450.1245711438373855No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5410.12365686021289093No Hit
GGCCGAGACAGTATTCTGAACAGGGCTCACTGTTCCTGAACTGGAGCCAC5380.12297114749452003No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT5350.12228543477614909No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC5320.12159972205777814No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAATCCCAAAAGACCCACAAT5320.12159972205777814No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA5300.12114258024553087No Hit
GCCACATCGTCAATCTTATCGAAGACTGGGGACCCTGTACCGAACATGGA5240.11977115480878901No Hit
CCAAAAGACACTAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA5240.11977115480878901No Hit
GTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCTTT5230.11954258390266537No Hit
CCTAACCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTGA5220.11931401299654172No Hit
GTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTGTACCGAA5170.1181711584659235No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA5140.11748544574755257No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5120.11702830393530529No Hit
CGATATGGCAGAGACAGTATTCTGAACAGGGCTCACTGTTCCTGAACTGG5100.116571162123058No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT5100.116571162123058No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5060.11565687849856345No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5050.1154283075924398No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC5010.1145140239679452No Hit
GTTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA4980.1138283112495743No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA4980.1138283112495743No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC4900.11199974400058514No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4810.10994260584547236No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA4800.10971403493934871No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT4740.10834260950260685No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT4740.10834260950260685No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAACCAGGAGACTA4730.1081140385964832No Hit
GCTTTAGGGCATGGACATTGACGCGTATAAAGAATTTGGAGCTTCTGTGG4700.10742832587811227No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4690.10719975497198865No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC4680.106971184065865No Hit
CCCCAACAGTCCCCCATGGGGAGGGGTGAACCCTAGCCCGAATGCTCCCA4640.10605690044137041No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACCCCCATAGGTAT4640.10605690044137041No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT4600.10514261681687585No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG4580.10468547500462856No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTACGTAAACAGTATC4530.10354262047401035No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGTGTCCAAGGAATACTAAC4530.10354262047401035No Hit
ATATATAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAA4530.10354262047401035No Hit
ACGTAAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC4480.10239976594339213No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCAGAACATTGTTCACCTCATCA4440.10148548231889755No Hit
GGGTAAAGGTTCAGATACTGTTTACGTAGAAAGGCCTTGTAAGTTGGCGA4410.10079976960052663No Hit
GCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC4400.10057119869440298No Hit
CTGTTTACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT4390.10034262778827933No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4390.10034262778827933No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)