FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437501
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA23360.5339416367048304No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCGAGTACAAAGACC17450.3988562311857573No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGACAGTCCAA14220.3250278285078205No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC12430.28411363631168846No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG12230.27954221818921554No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT11340.25919940754421134No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10830.2475422913319055No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT10670.24388515683392725No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT9960.2276566224991486No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG9680.22125663712768656No Hit
GTATCGGGAGGCCTTAGAGTCTCCAGAACATTGTTCACCTCATCATACGG8990.2054852446051552No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8720.19931383013981682No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC8530.19497098292346762No Hit
ATTCTGAACAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT8420.19245670295610753No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA8400.19199956114386024No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC8280.18925671027037652No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTATATATCCCATGAA8150.18628528849076917No Hit
ATATAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA7870.17988530311930714No Hit
GGATAATACGGTTTGATCCCTTTATCTAAAGGCAAATATTTAGTAACATT7840.17919959040093622No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG7710.17622816862132887No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT7520.17188532140497967No Hit
CTTTAGGGCATGGACATTGACGCGTATAAAGAATTTGGAGCTTCTGTGGA7510.171656750498856No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC7470.17074246687436143No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7250.16571390693964128No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7120.16274248516003392No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG7000.1599996342865502No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACTAAATATTCAAGAA6780.15497107435183005No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA6720.15359964891508818No Hit
CTCCAAAAGACACTAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA6630.1515425107599754No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCTGTGGGCGTTC6520.14902823079261532No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6510.1487996598864917No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA6420.1467425217313789No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT6380.14582823810688433No Hit
ATGTATCTGCGGCGTTTTATCATCTTCCTCTGCATCCTGCTGCTATGCCT6120.1398853945476696No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5970.13645683095581496No Hit
GCCTTAGAGTCTCCAGAACATTGTTCACCTCATCATACGGCACTCAGGCA5960.1362282600496913No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAAGGCAAATA5930.1355425473313204No Hit
GTTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA5840.1334854091762076No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5800.13257112555171302No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5740.13119970011497117No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC5730.13097112920884751No Hit
GTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCTTT5650.12914256195985838No Hit
TCTTATTGGTTCTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTA5650.12914256195985838No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC5590.1277711365231165No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5590.1277711365231165No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG5570.12731399471086924No Hit
AAAACTCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGA5550.12685685289862195No Hit
GTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTGTACCGAA5530.12639971108637466No Hit
GGCCGAGACAGTATTCTGAACAGGGCTCACTGTTCCTGAACTGGAGCCAC5480.12525685655575644No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA5430.12411400202513824No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5420.1238854311190146No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5420.1238854311190146No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAACCAGGAGACTA5410.12365686021289093No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGTACGACAGAAT5400.12342828930676729No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC5390.12319971840064364No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACTAAATATTCAA5380.12297114749452003No Hit
TAGCCGACTACTAATTCCCTGGATGCTGGATCTTCCAAATTACTTCCCAC5340.12205686387002544No Hit
CAGACACATCCAGCGATAACCAGGACAAATTGGAGGACAACAGGTTGGTG5240.11977115480878901No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC5220.11931401299654172No Hit
CCTAAAACTCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTG5210.11908544209041808No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA5180.11839972937204715No Hit
CCCCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG5110.11679973302918165No Hit
CCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGAAGATGATAAAA5080.11611402031081071No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5080.11611402031081071No Hit
GATGTATACTTGCGGGAGAGTACGACAGAATTGTCAGTCCCGATGAGTTT5070.11588544940468706No Hit
ATTATATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTG5000.11428545306182158No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAATCCCAAAAGACCCACAAT4980.1138283112495743No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT4940.11291402762507972No Hit
GTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4940.11291402762507972No Hit
CAATTATATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG4930.11268545671895608No Hit
GAAATGATGTATACTTGCGGGAGAGTACGACAGAATTGTCAGTCCCGATG4900.11199974400058514No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4880.11154260218833785No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA4830.11039974765771964No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4820.11017117675159599No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG4790.10948546403322507No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4770.10902832222097777No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT4730.1081140385964832No Hit
GGGTAAAGGTTCAGATACTGTTTACGTAGAAAGGCCTTGTAAGTTGGCGA4680.106971184065865No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT4670.10674261315974136No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC4640.10605690044137041No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4630.10582832953524676No Hit
GCTTTAGGGCATGGACATTGACGCGTATAAAGAATTTGGAGCTTCTGTGG4610.1053711877229995No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG4580.10468547500462856No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC4560.10422833319238128No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT4540.10377119138013399No Hit
CCCTTAACTTCATGGGATATATAATTGGGAGTTGGGGCACATTGCCACAG4520.1033140495678867No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC4520.1033140495678867No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA4500.10285690775563942No Hit
CCAAAAGACACTAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA4500.10285690775563942No Hit
CCTAACCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTGA4490.10262833684951578No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC4480.10239976594339213No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG4450.1017140532250212No Hit
CAAATCTTTCTGTCCCCAATCCCCTGGGATTCTTCCCCGATCATCAGTTG4440.10148548231889755No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4410.10079976960052663No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGTGTCCAAGGAATACTAAC4400.10057119869440298No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)